ID   RP22_FOWPN              Reviewed;         186 AA.
AC   Q85280;
DT   29-AUG-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 76.
DE   RecName: Full=DNA-directed RNA polymerase 22 kDa subunit;
DE            EC=2.7.7.6;
GN   Name=RPO22; OrderedLocusNames=FPV135;
OS   Fowlpox virus (strain NVSL) (FPV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Avipoxvirus.
OX   NCBI_TaxID=928301;
OH   NCBI_TaxID=7742; Vertebrata.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Salisbury;
RX   PubMed=2820129; DOI=10.1016/0042-6822(87)90061-4;
RA   Drillien R., Spehner D., Villeval D., Lecocq J.-P.;
RT   "Similar genetic organization between a region of fowlpox virus DNA and the
RT   vaccinia virus HindIII J fragment despite divergent location of the
RT   thymidine kinase gene.";
RL   Virology 160:203-209(1987).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=10729156; DOI=10.1128/jvi.74.8.3815-3831.2000;
RA   Afonso C.L., Tulman E.R., Lu Z., Zsak L., Kutish G.F., Rock D.L.;
RT   "The genome of fowlpox virus.";
RL   J. Virol. 74:3815-3831(2000).
CC   -!- FUNCTION: Part of the DNA-dependent RNA polymerase which catalyzes the
CC       transcription of viral DNA into RNA using the four ribonucleoside
CC       triphosphates as substrates. Responsible for the transcription of
CC       early, intermediate and late genes. DNA-dependent RNA polymerase
CC       associates with the early transcription factor (ETF) thereby allowing
CC       the early genes transcription. Late transcription, and probably also
CC       intermediate transcription, require newly synthesized RNA polymerase
CC       (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6;
CC   -!- SUBUNIT: The DNA-dependent RNA polymerase used for intermediate and
CC       late genes expression consists of eight subunits 147 kDa, 133 kDa, 35
CC       kDa, 30 kDa, 22 kDa, 19 kDa, 18 kDa and 7 kDa totalling more than 500
CC       kDa in mass. The same holoenzyme, with the addition of the
CC       transcription-specificity factor RAP94, is used for early gene
CC       expression (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}. Note=All the enzymes and
CC       other proteins required to synthesize early mRNAs are packaged within
CC       the virion core along with the DNA genome. This is necessary because
CC       viral early mRNAs are synthesized within minutes after virus entry into
CC       the cell and are extruded through pores in the core particle (By
CC       similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the poxviridae DNA-directed RNA polymerase 22
CC       kDa subunit family. {ECO:0000305}.
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DR   EMBL; M17418; AAA66423.1; -; Genomic_DNA.
DR   EMBL; AF198100; AAF44479.1; -; Genomic_DNA.
DR   RefSeq; NP_039098.1; NC_002188.1.
DR   SMR; Q85280; -.
DR   GeneID; 1486683; -.
DR   KEGG; vg:1486683; -.
DR   Proteomes; UP000008597; Genome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0019083; P:viral transcription; IEA:InterPro.
DR   InterPro; IPR007937; RNA_Pol_22kDa_poxvir.
DR   Pfam; PF05273; Pox_RNA_Pol_22; 1.
DR   PIRSF; PIRSF000744; RPO22; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transcription; Transferase; Virion.
FT   CHAIN           1..186
FT                   /note="DNA-directed RNA polymerase 22 kDa subunit"
FT                   /id="PRO_0000099142"
SQ   SEQUENCE   186 AA;  21800 MW;  584BD653E120FD97 CRC64;
     MMNQYNVTYL SKILCLKTEI LYKPFSIINR SIVNQYNIDV KYDDITSIVK IRHKTENTIL
     VFQIFNESNV KYSPIEYDYG DPIIITSNLQ HGHNRIPINM LYIDVVESDM FPTFSRLDSE
     TIKIITSILQ SDNKKEQSIK LPKVSENELS VKILYHKDYP LKYVRYYKNN MVTGIEVIDR
     SVAITS