ID   RP22_MYXVU              Reviewed;         185 AA.
AC   P68547; P18620;
DT   23-NOV-2004, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   03-AUG-2022, entry version 47.
DE   RecName: Full=DNA-directed RNA polymerase 21 kDa subunit;
DE            EC=2.7.7.6;
GN   Name=RPO22; Synonyms=POLR21; ORFNames=F10;
OS   Myxoma virus (strain Uriarra) (MYXV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Leporipoxvirus.
OX   NCBI_TaxID=265876;
OH   NCBI_TaxID=9986; Oryctolagus cuniculus (Rabbit).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2402456; DOI=10.1093/nar/18.17.5290;
RA   Jackson R.J., Bults G.H.;
RT   "A myxoma virus nucleotide sequence with homology to the vaccinia virus RNA
RT   polymerase 22-kDa subunit gene.";
RL   Nucleic Acids Res. 18:5290-5290(1990).
CC   -!- FUNCTION: Part of the DNA-dependent RNA polymerase which catalyzes the
CC       transcription of viral DNA into RNA using the four ribonucleoside
CC       triphosphates as substrates. Responsible for the transcription of
CC       early, intermediate and late genes. DNA-dependent RNA polymerase
CC       associates with the early transcription factor (ETF) thereby allowing
CC       the early genes transcription. Late transcription, and probably also
CC       intermediate transcription, require newly synthesized RNA polymerase
CC       (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6;
CC   -!- SUBUNIT: The DNA-dependent RNA polymerase used for intermediate and
CC       late genes expression consists of eight subunits 147 kDa, 133 kDa, 35
CC       kDa, 30 kDa, 22 kDa, 19 kDa, 18 kDa and 7 kDa totalling more than 500
CC       kDa in mass. The same holoenzyme, with the addition of the
CC       transcription-specificity factor RAP94, is used for early gene
CC       expression (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}. Note=All the enzymes and
CC       other proteins required to synthesize early mRNAs are packaged within
CC       the virion core along with the DNA genome. This is necessary because
CC       viral early mRNAs are synthesized within minutes after virus entry into
CC       the cell and are extruded through pores in the core particle (By
CC       similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the poxviridae DNA-directed RNA polymerase 22
CC       kDa subunit family. {ECO:0000305}.
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DR   EMBL; X17347; CAA35230.1; -; Genomic_DNA.
DR   PIR; S11242; S11242.
DR   RefSeq; NP_051780.1; NC_001132.2.
DR   SMR; P68547; -.
DR   PRIDE; P68547; -.
DR   GeneID; 932185; -.
DR   KEGG; vg:932185; -.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0019083; P:viral transcription; IEA:InterPro.
DR   InterPro; IPR007937; RNA_Pol_22kDa_poxvir.
DR   Pfam; PF05273; Pox_RNA_Pol_22; 1.
DR   PIRSF; PIRSF000744; RPO22; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW   Transferase; Virion.
FT   CHAIN           1..185
FT                   /note="DNA-directed RNA polymerase 21 kDa subunit"
FT                   /id="PRO_0000099144"
SQ   SEQUENCE   185 AA;  21026 MW;  2DD2FE1DCD9753D7 CRC64;
     MNPHNVKYLA KILCLKAEIQ KNPYAVISKD VVHRYSTDIR YGDLTTIISV RHKTSTSNTV
     FQVFNESSVN YTPVDNDYGY PIIITSFLQT GHNKFPISFL YIDVVASDVF PKFARLSPTD
     VATVYSVLQI GDTKDALKLP RMLETEISAK ILFHKDFPLK IVRFFKNNMV TGEEISDRSL
     VAVLE