RP22_VACCW
ID RP22_VACCW Reviewed; 185 AA.
AC P68609; P07391; Q76ZT0;
DT 01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1988, sequence version 1.
DT 03-AUG-2022, entry version 58.
DE RecName: Full=DNA-directed RNA polymerase 22 kDa subunit;
DE EC=2.7.7.6;
GN Name=RPO22; OrderedLocusNames=VACWR096; ORFNames=J4R;
OS Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS WR)).
OC Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX NCBI_TaxID=10254;
OH NCBI_TaxID=9913; Bos taurus (Bovine).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3517852; DOI=10.1073/pnas.83.10.3141;
RA Broyles S.S., Moss B.;
RT "Homology between RNA polymerases of poxviruses, prokaryotes, and
RT eukaryotes: nucleotide sequence and transcriptional analysis of vaccinia
RT virus genes encoding 147-kDa and 22-kDa subunits.";
RL Proc. Natl. Acad. Sci. U.S.A. 83:3141-3145(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2987815; DOI=10.1093/nar/13.3.985;
RA Plucienniczak A., Schroeder E., Zettlmeissl G., Streeck R.E.;
RT "Nucleotide sequence of a cluster of early and late genes in a conserved
RT segment of the vaccinia virus genome.";
RL Nucleic Acids Res. 13:985-998(1985).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA Wohlhueter R.;
RT "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT redundancy and an error rate of 0.16/10kb.";
RL Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP REVIEW.
RX PubMed=12917449; DOI=10.1099/vir.0.18942-0;
RA Broyles S.S.;
RT "Vaccinia virus transcription.";
RL J. Gen. Virol. 84:2293-2303(2003).
RN [5]
RP FUNCTION.
RX PubMed=19759131; DOI=10.1128/jvi.01653-09;
RA Yang Z., Moss B.;
RT "Interaction of the vaccinia virus RNA polymerase-associated 94-kilodalton
RT protein with the early transcription factor.";
RL J. Virol. 83:12018-12026(2009).
CC -!- FUNCTION: Part of the DNA-dependent RNA polymerase which catalyzes the
CC transcription of viral DNA into RNA using the four ribonucleoside
CC triphosphates as substrates. Responsible for the transcription of
CC early, intermediate and late genes. DNA-dependent RNA polymerase
CC associates with the early transcription factor (ETF), itself composed
CC of D6 and A7, thereby allowing the early genes transcription. Late
CC transcription, and probably also intermediate transcription, require
CC newly synthesized RNA polymerase. {ECO:0000269|PubMed:19759131}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6;
CC -!- SUBUNIT: The DNA-dependent RNA polymerase used for intermediate and
CC late genes expression consists of eight subunits 147 kDa, 133 kDa, 35
CC kDa, 30 kDa, 22 kDa, 19 kDa, 18 kDa and 7 kDa totalling more than 500
CC kDa in mass. The same holoenzyme, with the addition of the
CC transcription-specificity factor RAP94, is used for early gene
CC expression.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}. Note=All the enzymes and
CC other proteins required to synthesize early mRNAs are packaged within
CC the virion core along with the DNA genome. This is necessary because
CC viral early mRNAs are synthesized within minutes after virus entry into
CC the cell and are extruded through pores in the core particle.
CC -!- SIMILARITY: Belongs to the poxviridae DNA-directed RNA polymerase 22
CC kDa subunit family. {ECO:0000305}.
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DR EMBL; M13209; AAB59833.1; -; Genomic_DNA.
DR EMBL; X01978; CAA26018.1; -; Genomic_DNA.
DR EMBL; AY243312; AAO89375.1; -; Genomic_DNA.
DR PIR; A25734; RNVZ22.
DR RefSeq; YP_232978.1; NC_006998.1.
DR PDB; 6RIE; EM; 3.10 A; E=1-185.
DR PDBsum; 6RIE; -.
DR SMR; P68609; -.
DR DNASU; 3707552; -.
DR GeneID; 3707552; -.
DR KEGG; vg:3707552; -.
DR Proteomes; UP000000344; Genome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0039695; P:DNA-templated viral transcription; NAS:UniProtKB.
DR InterPro; IPR007937; RNA_Pol_22kDa_poxvir.
DR Pfam; PF05273; Pox_RNA_Pol_22; 1.
DR PIRSF; PIRSF000744; RPO22; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-directed RNA polymerase; Nucleotidyltransferase;
KW Reference proteome; Transcription; Transferase; Virion.
FT CHAIN 1..185
FT /note="DNA-directed RNA polymerase 22 kDa subunit"
FT /id="PRO_0000099140"
FT HELIX 5..21
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 32..34
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 35..40
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 46..52
FT /evidence="ECO:0007829|PDB:6RIE"
FT TURN 53..55
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 59..67
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 75..84
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 92..95
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 113..115
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 118..127
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 141..146
FT /evidence="ECO:0007829|PDB:6RIE"
FT HELIX 147..151
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 155..157
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 161..168
FT /evidence="ECO:0007829|PDB:6RIE"
FT TURN 169..171
FT /evidence="ECO:0007829|PDB:6RIE"
FT STRAND 172..181
FT /evidence="ECO:0007829|PDB:6RIE"
SQ SEQUENCE 185 AA; 21342 MW; E4A289D674B3B9E0 CRC64;
MNQYNVKYLA KILCLKTEIA RDPYAVINRN VLLRYTTDIE YNDLVTLITV RHKIDSMKTV
FQVFNESSIN YTPVDDDYGE PIIITSYLQK GHNKFPVNFL YIDVVISDLF PSFVRLDTTE
TNIVNSVLQT GDGKKTLRLP KMLETEIVVK ILYRPNIPLK IVRFFRNNMV TGVEIADRSV
ISVAD
