ID   RP28_BACAN              Reviewed;         237 AA.
AC   P62182; P26765; Q6HT54; Q6KME2;
DT   21-JUN-2004, integrated into UniProtKB/Swiss-Prot.
DT   21-JUN-2004, sequence version 1.
DT   25-MAY-2022, entry version 122.
DE   RecName: Full=RNA polymerase sigma-28 factor;
DE   Flags: Precursor;
GN   Name=sigK; OrderedLocusNames=BA_4566, GBAA_4566, BAS4236;
OS   Bacillus anthracis.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=1392;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Ames / isolate Porton;
RX   PubMed=12721629; DOI=10.1038/nature01586;
RA   Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T.,
RA   Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R.,
RA   Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M.,
RA   Kolonay J.F., Beanan M.J., Dodson R.J., Brinkac L.M., Gwinn M.L.,
RA   DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C.,
RA   Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y.,
RA   Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M.,
RA   Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E.,
RA   White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M.,
RA   Hanna P.C., Kolstoe A.-B., Fraser C.M.;
RT   "The genome sequence of Bacillus anthracis Ames and comparison to closely
RT   related bacteria.";
RL   Nature 423:81-86(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Ames ancestor;
RX   PubMed=18952800; DOI=10.1128/jb.01347-08;
RA   Ravel J., Jiang L., Stanley S.T., Wilson M.R., Decker R.S., Read T.D.,
RA   Worsham P., Keim P.S., Salzberg S.L., Fraser-Liggett C.M., Rasko D.A.;
RT   "The complete genome sequence of Bacillus anthracis Ames 'Ancestor'.";
RL   J. Bacteriol. 191:445-446(2009).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Sterne;
RA   Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K.,
RA   Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R.,
RA   Richardson P., Rubin E., Tice H.;
RT   "Complete genome sequence of Bacillus anthracis Sterne.";
RL   Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. This sigma factor directs the transcription of crystal
CC       protein genes, a sporulation-regulated event (By similarity).
CC       {ECO:0000250}.
CC   -!- PTM: Proteolytically cleaved in the N-terminus probably by a SpoIIGA
CC       homolog to yield the active peptide. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE016879; AAP28274.1; -; Genomic_DNA.
DR   EMBL; AE017334; AAT33686.1; -; Genomic_DNA.
DR   EMBL; AE017225; AAT56535.1; -; Genomic_DNA.
DR   RefSeq; NP_846788.1; NC_003997.3.
DR   RefSeq; WP_000051382.1; NZ_WXXJ01000027.1.
DR   RefSeq; YP_030484.1; NC_005945.1.
DR   AlphaFoldDB; P62182; -.
DR   SMR; P62182; -.
DR   STRING; 260799.BAS4236; -.
DR   DNASU; 1088335; -.
DR   EnsemblBacteria; AAP28274; AAP28274; BA_4566.
DR   EnsemblBacteria; AAT33686; AAT33686; GBAA_4566.
DR   GeneID; 59155089; -.
DR   GeneID; 64199684; -.
DR   GeneID; 67508976; -.
DR   KEGG; ban:BA_4566; -.
DR   KEGG; bar:GBAA_4566; -.
DR   KEGG; bat:BAS4236; -.
DR   PATRIC; fig|198094.11.peg.4532; -.
DR   eggNOG; COG1191; Bacteria.
DR   HOGENOM; CLU_014793_8_7_9; -.
DR   OMA; LMYLRQN; -.
DR   Proteomes; UP000000427; Chromosome.
DR   Proteomes; UP000000594; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR001387; Cro/C1-type_HTH.
DR   InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR   InterPro; IPR014209; RNA_pol_sigma-K.
DR   InterPro; IPR000943; RNA_pol_sigma70.
DR   InterPro; IPR007627; RNA_pol_sigma70_r2.
DR   InterPro; IPR007630; RNA_pol_sigma70_r4.
DR   InterPro; IPR013325; RNA_pol_sigma_r2.
DR   InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF04542; Sigma70_r2; 1.
DR   Pfam; PF04545; Sigma70_r4; 1.
DR   PIRSF; PIRSF000770; RNA_pol_sigma-SigE/K; 1.
DR   PRINTS; PR00046; SIGMA70FCT.
DR   SUPFAM; SSF88659; SSF88659; 1.
DR   SUPFAM; SSF88946; SSF88946; 1.
DR   TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR   TIGRFAMs; TIGR02846; spore_sigmaK; 1.
DR   PROSITE; PS00715; SIGMA70_1; 1.
DR   PROSITE; PS00716; SIGMA70_2; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Reference proteome; Sigma factor; Sporulation; Transcription;
KW   Transcription regulation.
FT   PROPEP          1..19
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000032590"
FT   CHAIN           20..237
FT                   /note="RNA polymerase sigma-28 factor"
FT                   /id="PRO_0000032591"
FT   DNA_BIND        197..206
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   MOTIF           77..90
FT                   /note="Polymerase core binding"
SQ   SEQUENCE   237 AA;  27136 MW;  C1D8E4EB637CEDA5 CRC64;
     MSLFAAIGYM VREVFVFVSY VKNNAFPQPL SSDDERKYLE LMEQGDAQAR NLLIEHNLRL
     VAHIVKKFEN TGEDAEDLIS IGTIGLIKAI ESYSAGKGTK LATYAARCIE NEILMHLRVL
     KKTKKDVSLH DPIGQDKEGN EISLIDILKS ESEDVIDMIQ LSMELEKIKE YIDILDEREK
     EVIVKRFGLG LDKEKTQREI AKALGISRSY VSRIEKRALM KMFHEFVRAE KEKKAKE