RP28_BACTK
ID RP28_BACTK Reviewed; 237 AA.
AC P62181; P26765;
DT 21-JUN-2004, integrated into UniProtKB/Swiss-Prot.
DT 21-JUN-2004, sequence version 1.
DT 25-MAY-2022, entry version 77.
DE RecName: Full=RNA polymerase sigma-28 factor;
DE Flags: Precursor;
GN Name=sigK;
OS Bacillus thuringiensis subsp. kurstaki.
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC Bacillus cereus group.
OX NCBI_TaxID=29339;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 20-37.
RC STRAIN=HD-1;
RX PubMed=1904859; DOI=10.1128/jb.173.12.3846-3854.1991;
RA Adams L.F., Brown K.L., Whiteley H.R.;
RT "Molecular cloning and characterization of two genes encoding sigma factors
RT that direct transcription from a Bacillus thuringiensis crystal protein
RT gene promoter.";
RL J. Bacteriol. 173:3846-3854(1991).
RN [2]
RP CHARACTERIZATION.
RX PubMed=1701426; DOI=10.1128/jb.172.12.6682-6688.1990;
RA Brown K.L., Whiteley H.R.;
RT "Isolation of the second Bacillus thuringiensis RNA polymerase that
RT transcribes from a crystal protein gene promoter.";
RL J. Bacteriol. 172:6682-6688(1990).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. This sigma factor directs the transcription of crystal
CC protein genes, a sporulation-regulated event.
CC -!- DEVELOPMENTAL STAGE: Is probably activated at the time immediately
CC preceding spore cortex formation (stage IV).
CC -!- PTM: Proteolytically cleaved in the N-terminus probably by a SpoIIGA
CC homolog to yield the active peptide. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X56696; CAA40024.1; -; Genomic_DNA.
DR PIR; A39441; A39441.
DR RefSeq; WP_000051382.1; NZ_PHSM01000001.1.
DR AlphaFoldDB; P62181; -.
DR SMR; P62181; -.
DR GeneID; 59155089; -.
DR GeneID; 64199684; -.
DR GeneID; 67508976; -.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR001387; Cro/C1-type_HTH.
DR InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR InterPro; IPR014209; RNA_pol_sigma-K.
DR InterPro; IPR000943; RNA_pol_sigma70.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR007630; RNA_pol_sigma70_r4.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF04542; Sigma70_r2; 1.
DR Pfam; PF04545; Sigma70_r4; 1.
DR PIRSF; PIRSF000770; RNA_pol_sigma-SigE/K; 1.
DR PRINTS; PR00046; SIGMA70FCT.
DR SUPFAM; SSF88659; SSF88659; 1.
DR SUPFAM; SSF88946; SSF88946; 1.
DR TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR TIGRFAMs; TIGR02846; spore_sigmaK; 1.
DR PROSITE; PS00715; SIGMA70_1; 1.
DR PROSITE; PS00716; SIGMA70_2; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; DNA-binding; Sigma factor; Sporulation;
KW Transcription; Transcription regulation.
FT PROPEP 1..19
FT /evidence="ECO:0000269|PubMed:1904859"
FT /id="PRO_0000032594"
FT CHAIN 20..237
FT /note="RNA polymerase sigma-28 factor"
FT /id="PRO_0000032595"
FT DNA_BIND 197..206
FT /note="H-T-H motif"
FT /evidence="ECO:0000250"
FT MOTIF 77..90
FT /note="Polymerase core binding"
SQ SEQUENCE 237 AA; 27136 MW; C1D8E4EB637CEDA5 CRC64;
MSLFAAIGYM VREVFVFVSY VKNNAFPQPL SSDDERKYLE LMEQGDAQAR NLLIEHNLRL
VAHIVKKFEN TGEDAEDLIS IGTIGLIKAI ESYSAGKGTK LATYAARCIE NEILMHLRVL
KKTKKDVSLH DPIGQDKEGN EISLIDILKS ESEDVIDMIQ LSMELEKIKE YIDILDEREK
EVIVKRFGLG LDKEKTQREI AKALGISRSY VSRIEKRALM KMFHEFVRAE KEKKAKE
