ID   RP35_BACAN              Reviewed;         239 AA.
AC   P62177; P26763; Q6HUI2; Q6KNR8;
DT   21-JUN-2004, integrated into UniProtKB/Swiss-Prot.
DT   21-JUN-2004, sequence version 1.
DT   25-MAY-2022, entry version 119.
DE   RecName: Full=RNA polymerase sigma-35 factor;
DE   Flags: Precursor;
GN   Name=sigE; OrderedLocusNames=BA_4043, GBAA_4043, BAS3755;
OS   Bacillus anthracis.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=1392;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Ames / isolate Porton;
RX   PubMed=12721629; DOI=10.1038/nature01586;
RA   Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T.,
RA   Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R.,
RA   Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M.,
RA   Kolonay J.F., Beanan M.J., Dodson R.J., Brinkac L.M., Gwinn M.L.,
RA   DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C.,
RA   Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y.,
RA   Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M.,
RA   Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E.,
RA   White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M.,
RA   Hanna P.C., Kolstoe A.-B., Fraser C.M.;
RT   "The genome sequence of Bacillus anthracis Ames and comparison to closely
RT   related bacteria.";
RL   Nature 423:81-86(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Ames ancestor;
RX   PubMed=18952800; DOI=10.1128/jb.01347-08;
RA   Ravel J., Jiang L., Stanley S.T., Wilson M.R., Decker R.S., Read T.D.,
RA   Worsham P., Keim P.S., Salzberg S.L., Fraser-Liggett C.M., Rasko D.A.;
RT   "The complete genome sequence of Bacillus anthracis Ames 'Ancestor'.";
RL   J. Bacteriol. 191:445-446(2009).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Sterne;
RA   Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K.,
RA   Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R.,
RA   Richardson P., Rubin E., Tice H.;
RT   "Complete genome sequence of Bacillus anthracis Sterne.";
RL   Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. This sigma factor directs the transcription of crystal
CC       protein genes, a sporulation-regulated event (By similarity).
CC       {ECO:0000250}.
CC   -!- PTM: Proteolytically cleaved in the N-terminus probably by a SpoIIGA
CC       homolog to yield the active peptide. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. {ECO:0000305}.
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DR   EMBL; AE016879; AAP27769.1; -; Genomic_DNA.
DR   EMBL; AE017334; AAT33160.1; -; Genomic_DNA.
DR   EMBL; AE017225; AAT56057.1; -; Genomic_DNA.
DR   RefSeq; NP_846283.1; NC_003997.3.
DR   RefSeq; WP_000976948.1; NZ_WXXJ01000026.1.
DR   RefSeq; YP_030006.1; NC_005945.1.
DR   AlphaFoldDB; P62177; -.
DR   SMR; P62177; -.
DR   STRING; 260799.BAS3755; -.
DR   DNASU; 1086623; -.
DR   EnsemblBacteria; AAP27769; AAP27769; BA_4043.
DR   EnsemblBacteria; AAT33160; AAT33160; GBAA_4043.
DR   GeneID; 59155595; -.
DR   GeneID; 64199245; -.
DR   GeneID; 67508538; -.
DR   KEGG; ban:BA_4043; -.
DR   KEGG; bar:GBAA_4043; -.
DR   KEGG; bat:BAS3755; -.
DR   PATRIC; fig|198094.11.peg.4014; -.
DR   eggNOG; COG1191; Bacteria.
DR   HOGENOM; CLU_014793_8_7_9; -.
DR   OMA; EPLNTDW; -.
DR   Proteomes; UP000000427; Chromosome.
DR   Proteomes; UP000000594; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR001387; Cro/C1-type_HTH.
DR   InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR   InterPro; IPR014200; RNA_pol_sigma-E.
DR   InterPro; IPR000943; RNA_pol_sigma70.
DR   InterPro; IPR007627; RNA_pol_sigma70_r2.
DR   InterPro; IPR007630; RNA_pol_sigma70_r4.
DR   InterPro; IPR013325; RNA_pol_sigma_r2.
DR   InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF04542; Sigma70_r2; 1.
DR   Pfam; PF04545; Sigma70_r4; 1.
DR   PIRSF; PIRSF000770; RNA_pol_sigma-SigE/K; 1.
DR   PRINTS; PR00046; SIGMA70FCT.
DR   SUPFAM; SSF88659; SSF88659; 1.
DR   SUPFAM; SSF88946; SSF88946; 1.
DR   TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR   TIGRFAMs; TIGR02835; spore_sigmaE; 1.
DR   PROSITE; PS00715; SIGMA70_1; 1.
DR   PROSITE; PS00716; SIGMA70_2; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Reference proteome; Sigma factor; Sporulation; Transcription;
KW   Transcription regulation.
FT   PROPEP          1..27
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000032582"
FT   CHAIN           28..239
FT                   /note="RNA polymerase sigma-35 factor"
FT                   /id="PRO_0000032583"
FT   DNA_BIND        206..225
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   MOTIF           86..99
FT                   /note="Polymerase core binding"
SQ   SEQUENCE   239 AA;  27636 MW;  FF552F918F933F24 CRC64;
     MMKLKFYLVY LWYKVLLKLG IKTDEIYYIG GSEALPPPLT KEEEEVLLNK LPKGDQAARS
     LLIERNLRLV VYIARKFENT GINIEDLISI GTIGLIKAVN TFNPEKKIKL ATYASRCIEN
     EILMHLRRNN KNRSEVSFDE PLNIDWDGNE LLLSDVLGTD DDIITKDLEA TVDRHLLMKA
     LHQLNDREKQ IMELRFGLAG GEEKTQKDVA DMLGISQSYI SRLEKRIIKR LRKEFNKMV