ID   RP35_BACTK              Reviewed;         239 AA.
AC   P62179; P26763;
DT   21-JUN-2004, integrated into UniProtKB/Swiss-Prot.
DT   21-JUN-2004, sequence version 1.
DT   25-MAY-2022, entry version 77.
DE   RecName: Full=RNA polymerase sigma-35 factor;
DE   Flags: Precursor;
GN   Name=sigE;
OS   Bacillus thuringiensis subsp. kurstaki.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=29339;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 28-52.
RC   STRAIN=HD-1;
RX   PubMed=1904859; DOI=10.1128/jb.173.12.3846-3854.1991;
RA   Adams L.F., Brown K.L., Whiteley H.R.;
RT   "Molecular cloning and characterization of two genes encoding sigma factors
RT   that direct transcription from a Bacillus thuringiensis crystal protein
RT   gene promoter.";
RL   J. Bacteriol. 173:3846-3854(1991).
RN   [2]
RP   CHARACTERIZATION.
RX   PubMed=1701426; DOI=10.1128/jb.172.12.6682-6688.1990;
RA   Brown K.L., Whiteley H.R.;
RT   "Isolation of the second Bacillus thuringiensis RNA polymerase that
RT   transcribes from a crystal protein gene promoter.";
RL   J. Bacteriol. 172:6682-6688(1990).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. This sigma factor directs the transcription of crystal
CC       protein genes, a sporulation-regulated event.
CC   -!- DEVELOPMENTAL STAGE: Is probably activated at the time of septum
CC       formation separating the mother cell from the nascent endospore, (stage
CC       II).
CC   -!- PTM: Proteolytically cleaved in the N-terminus probably by a SpoIIGA
CC       homolog to yield the active peptide. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. {ECO:0000305}.
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DR   EMBL; X56697; CAA40026.1; -; Genomic_DNA.
DR   PIR; B39441; B39441.
DR   RefSeq; WP_000976948.1; NZ_PHSM01000001.1.
DR   AlphaFoldDB; P62179; -.
DR   SMR; P62179; -.
DR   GeneID; 59155595; -.
DR   GeneID; 64199245; -.
DR   GeneID; 67508538; -.
DR   OMA; EPLNTDW; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR001387; Cro/C1-type_HTH.
DR   InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR   InterPro; IPR014200; RNA_pol_sigma-E.
DR   InterPro; IPR000943; RNA_pol_sigma70.
DR   InterPro; IPR007627; RNA_pol_sigma70_r2.
DR   InterPro; IPR007630; RNA_pol_sigma70_r4.
DR   InterPro; IPR013325; RNA_pol_sigma_r2.
DR   InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF04542; Sigma70_r2; 1.
DR   Pfam; PF04545; Sigma70_r4; 1.
DR   PIRSF; PIRSF000770; RNA_pol_sigma-SigE/K; 1.
DR   PRINTS; PR00046; SIGMA70FCT.
DR   SUPFAM; SSF88659; SSF88659; 1.
DR   SUPFAM; SSF88946; SSF88946; 1.
DR   TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR   TIGRFAMs; TIGR02835; spore_sigmaE; 1.
DR   PROSITE; PS00715; SIGMA70_1; 1.
DR   PROSITE; PS00716; SIGMA70_2; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; DNA-binding; Sigma factor; Sporulation;
KW   Transcription; Transcription regulation.
FT   PROPEP          1..27
FT                   /evidence="ECO:0000269|PubMed:1904859"
FT                   /id="PRO_0000032586"
FT   CHAIN           28..239
FT                   /note="RNA polymerase sigma-35 factor"
FT                   /id="PRO_0000032587"
FT   DNA_BIND        206..225
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   MOTIF           86..99
FT                   /note="Polymerase core binding"
FT   CONFLICT        45
FT                   /note="E -> Y (in Ref. 1; AA sequence)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   239 AA;  27636 MW;  FF552F918F933F24 CRC64;
     MMKLKFYLVY LWYKVLLKLG IKTDEIYYIG GSEALPPPLT KEEEEVLLNK LPKGDQAARS
     LLIERNLRLV VYIARKFENT GINIEDLISI GTIGLIKAVN TFNPEKKIKL ATYASRCIEN
     EILMHLRRNN KNRSEVSFDE PLNIDWDGNE LLLSDVLGTD DDIITKDLEA TVDRHLLMKA
     LHQLNDREKQ IMELRFGLAG GEEKTQKDVA DMLGISQSYI SRLEKRIIKR LRKEFNKMV