ID   RP35_RABPU              Reviewed;         305 AA.
AC   Q6RZF4;
DT   07-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 39.
DE   RecName: Full=DNA-directed RNA polymerase 35 kDa subunit;
DE            EC=2.7.7.6;
GN   Name=RPO35; OrderedLocusNames=RPXV137;
OS   Rabbitpox virus (strain Utrecht) (RPV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=45417;
OH   NCBI_TaxID=9986; Oryctolagus cuniculus (Rabbit).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16227218; DOI=10.1099/vir.0.81331-0;
RA   Li G., Chen N., Roper R.L., Feng Z., Hunter A.L., Danila M.,
RA   Lefkowitz E.J., Buller R.M.L., Upton C.;
RT   "Complete coding sequences of the rabbitpox virus genome.";
RL   J. Gen. Virol. 86:2969-2977(2005).
CC   -!- FUNCTION: Part of the DNA-dependent RNA polymerase which catalyzes the
CC       transcription of viral DNA into RNA using the four ribonucleoside
CC       triphosphates as substrates. Responsible for the transcription of
CC       early, intermediate and late genes. DNA-dependent RNA polymerase
CC       associates with the early transcription factor (ETF) thereby allowing
CC       the early genes transcription. Late transcription, and probably also
CC       intermediate transcription, require newly synthesized RNA polymerase
CC       (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6;
CC   -!- SUBUNIT: The DNA-dependent RNA polymerase used for intermediate and
CC       late genes expression consists of eight subunits 147 kDa, 133 kDa, 35
CC       kDa, 30 kDa, 22 kDa, 19 kDa, 18 kDa and 7 kDa totalling more than 500
CC       kDa in mass. The same holoenzyme, with the addition of the
CC       transcription-specificity factor RAP94, is used for early gene
CC       expression (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}. Note=All the enzymes and
CC       other proteins required to synthesize early mRNAs are packaged within
CC       the virion core along with the DNA genome. This is necessary because
CC       viral early mRNAs are synthesized within minutes after virus entry into
CC       the cell and are extruded through pores in the core particle (By
CC       similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the poxviridae DNA-directed RNA polymerase 35
CC       kDa subunit family. {ECO:0000305}.
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DR   EMBL; AY484669; AAS49850.1; -; Genomic_DNA.
DR   SMR; Q6RZF4; -.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0019083; P:viral transcription; IEA:InterPro.
DR   InterPro; IPR005059; DNA-dir_RNA_pol_35kDa_poxviral.
DR   Pfam; PF03396; Pox_RNA_pol_35; 1.
DR   PIRSF; PIRSF000746; Rpo35; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW   Transferase; Virion.
FT   CHAIN           1..305
FT                   /note="DNA-directed RNA polymerase 35 kDa subunit"
FT                   /id="PRO_0000099138"
SQ   SEQUENCE   305 AA;  35334 MW;  64F1FE9F55A72E26 CRC64;
     MQHPREENSI VVELEPSLAT FIKQGFNNLV KWPLLNIGIV LSNTSTAVNE EWLTAVEHIP
     TMKIFYKHIH KILTREMGFL VYLKRSQSER DNYITLYDFD YYIIDKDTNS VTMVDKPTEL
     KETLLHVFQE YRLKSSQTIE LIAFSSGTVI NEDIVSKLTF LDVEVFNREY NNVKTIIDPD
     FVSRSPFIVI SPMGKLTFFV EVYSWFDFKS CFKDIIDFLE GALIANIHNH MIKVGDCDET
     VSSYNPESGM LFVNDLMTMN IVNFFGCNSR LESYHRFDMT KVDVELFIKA LSDACKKILS
     ASNRL