RP35_SHEVK
ID RP35_SHEVK Reviewed; 126 AA.
AC P19749;
DT 01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1991, sequence version 1.
DT 03-AUG-2022, entry version 56.
DE RecName: Full=DNA-directed RNA polymerase 35 kDa subunit;
DE EC=2.7.7.6;
DE Flags: Fragment;
GN Name=RPO35; ORFNames=HM4;
OS Sheeppox virus (strain KS-1) (SPPV) (Capripoxvirus (strain KS-1)).
OC Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC Chitovirales; Poxviridae; Chordopoxvirinae; Capripoxvirus.
OX NCBI_TaxID=10269;
OH NCBI_TaxID=9940; Ovis aries (Sheep).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2795717; DOI=10.1128/jvi.63.11.4703-4708.1989;
RA Gershon P.D., Ansell D.M., Black D.N.;
RT "A comparison of the genome organization of capripoxvirus with that of the
RT orthopoxviruses.";
RL J. Virol. 63:4703-4708(1989).
CC -!- FUNCTION: Part of the DNA-dependent RNA polymerase which catalyzes the
CC transcription of viral DNA into RNA using the four ribonucleoside
CC triphosphates as substrates. Responsible for the transcription of
CC early, intermediate and late genes. DNA-dependent RNA polymerase
CC associates with the early transcription factor (ETF) thereby allowing
CC the early genes transcription. Late transcription, and probably also
CC intermediate transcription, require newly synthesized RNA polymerase
CC (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6;
CC -!- SUBUNIT: The DNA-dependent RNA polymerase used for intermediate and
CC late genes expression consists of eight subunits 147 kDa, 133 kDa, 35
CC kDa, 30 kDa, 22 kDa, 19 kDa, 18 kDa and 7 kDa totalling more than 500
CC kDa in mass. The same holoenzyme, with the addition of the
CC transcription-specificity factor RAP94, is used for early gene
CC expression (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}. Note=All the enzymes and
CC other proteins required to synthesize early mRNAs are packaged within
CC the virion core along with the DNA genome. This is necessary because
CC viral early mRNAs are synthesized within minutes after virus entry into
CC the cell and are extruded through pores in the core particle (By
CC similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the poxviridae DNA-directed RNA polymerase 35
CC kDa subunit family. {ECO:0000305}.
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DR EMBL; M30039; AAC32900.1; -; Genomic_DNA.
DR PIR; D33325; WMVZM4.
DR SMR; P19749; -.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0019083; P:viral transcription; IEA:InterPro.
DR InterPro; IPR005059; DNA-dir_RNA_pol_35kDa_poxviral.
DR Pfam; PF03396; Pox_RNA_pol_35; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW Transferase; Virion.
FT CHAIN <1..126
FT /note="DNA-directed RNA polymerase 35 kDa subunit"
FT /id="PRO_0000099136"
FT NON_TER 1
SQ SEQUENCE 126 AA; 14408 MW; AE25E13BEFF80F7B CRC64;
SFESIIPFIV TAPLGRLTFY VEHYPWIDFK SHMKEILDFL EGSLVSDVHS HKLETSVLDN
STSSSYNPVS GMLFVNDLLT MTVVNFFGCN SRLNSYHRFD MTKLDINTFL KALADAFKKI
ANYLEV