ID   RP35_VACCA              Reviewed;         305 AA.
AC   O57233; Q80HU5;
DT   07-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   03-AUG-2022, entry version 66.
DE   RecName: Full=DNA-directed RNA polymerase 35 kDa subunit;
DE            EC=2.7.7.6;
GN   Name=RPO35; OrderedLocusNames=MVA140L, ACAM3000_MVA_140;
OS   Vaccinia virus (strain Ankara) (VACV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=126794;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=9601507; DOI=10.1006/viro.1998.9123;
RA   Antoine G., Scheiflinger F., Dorner F., Falkner F.G.;
RT   "The complete genomic sequence of the modified vaccinia Ankara strain:
RT   comparison with other orthopoxviruses.";
RL   Virology 244:365-396(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Isolate Acambis 3000;
RA   Esposito J.J., Frace M., Sammons S.A., Olsen-Rasmussen M.S., Osborne J.,
RA   Khristova M., Wohlhueter R.M.;
RL   Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   REVIEW.
RX   PubMed=12917449; DOI=10.1099/vir.0.18942-0;
RA   Broyles S.S.;
RT   "Vaccinia virus transcription.";
RL   J. Gen. Virol. 84:2293-2303(2003).
CC   -!- FUNCTION: Part of the DNA-dependent RNA polymerase which catalyzes the
CC       transcription of viral DNA into RNA using the four ribonucleoside
CC       triphosphates as substrates. Responsible for the transcription of
CC       early, intermediate and late genes. DNA-dependent RNA polymerase
CC       associates with the early transcription factor (ETF), itself composed
CC       of D6 and A7, thereby allowing the early genes transcription. Late
CC       transcription, and probably also intermediate transcription, require
CC       newly synthesized RNA polymerase.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6;
CC   -!- SUBUNIT: The DNA-dependent RNA polymerase used for intermediate and
CC       late genes expression consists of eight subunits 147 kDa, 133 kDa, 35
CC       kDa, 30 kDa, 22 kDa, 19 kDa, 18 kDa and 7 kDa totalling more than 500
CC       kDa in mass. The same holoenzyme, with the addition of the
CC       transcription-specificity factor RAP94, is used for early gene
CC       expression.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}. Note=All the enzymes and
CC       other proteins required to synthesize early mRNAs are packaged within
CC       the virion core along with the DNA genome. This is necessary because
CC       viral early mRNAs are synthesized within minutes after virus entry into
CC       the cell and are extruded through pores in the core particle.
CC   -!- SIMILARITY: Belongs to the poxviridae DNA-directed RNA polymerase 35
CC       kDa subunit family. {ECO:0000305}.
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DR   EMBL; U94848; AAB96474.1; -; Genomic_DNA.
DR   EMBL; AY603355; AAT10538.1; -; Genomic_DNA.
DR   PIR; T37415; T37415.
DR   RefSeq; YP_233034.1; NC_006998.1.
DR   PDB; 6RIE; EM; 3.10 A; C=1-305.
DR   PDBsum; 6RIE; -.
DR   SMR; O57233; -.
DR   DNASU; 3707682; -.
DR   GeneID; 3707682; -.
DR   KEGG; vg:3707682; -.
DR   Proteomes; UP000159908; Genome.
DR   Proteomes; UP000172909; Genome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0019083; P:viral transcription; IEA:InterPro.
DR   InterPro; IPR005059; DNA-dir_RNA_pol_35kDa_poxviral.
DR   Pfam; PF03396; Pox_RNA_pol_35; 1.
DR   PIRSF; PIRSF000746; Rpo35; 1.
PE   1: Evidence at protein level;
KW   3D-structure; DNA-directed RNA polymerase; Nucleotidyltransferase;
KW   Transcription; Transferase; Virion.
FT   CHAIN           1..305
FT                   /note="DNA-directed RNA polymerase 35 kDa subunit"
FT                   /id="PRO_0000099131"
FT   STRAND          5..13
FT                   /evidence="ECO:0007829|PDB:6RIE"
FT   HELIX           16..29
FT                   /evidence="ECO:0007829|PDB:6RIE"
FT   STRAND          37..43
FT                   /evidence="ECO:0007829|PDB:6RIE"
FT   STRAND          45..47
FT                   /evidence="ECO:0007829|PDB:6RIE"
FT   HELIX           49..56
FT                   /evidence="ECO:0007829|PDB:6RIE"
FT   TURN            62..64
FT                   /evidence="ECO:0007829|PDB:6RIE"
FT   HELIX           66..68
FT                   /evidence="ECO:0007829|PDB:6RIE"
FT   HELIX           69..73
FT                   /evidence="ECO:0007829|PDB:6RIE"
FT   STRAND          76..86
FT                   /evidence="ECO:0007829|PDB:6RIE"
FT   STRAND          89..91
FT                   /evidence="ECO:0007829|PDB:6RIE"
FT   STRAND          96..105
FT                   /evidence="ECO:0007829|PDB:6RIE"
FT   TURN            106..109
FT                   /evidence="ECO:0007829|PDB:6RIE"
FT   STRAND          110..113
FT                   /evidence="ECO:0007829|PDB:6RIE"
FT   HELIX           118..121
FT                   /evidence="ECO:0007829|PDB:6RIE"
FT   STRAND          125..127
FT                   /evidence="ECO:0007829|PDB:6RIE"
FT   STRAND          133..135
FT                   /evidence="ECO:0007829|PDB:6RIE"
FT   STRAND          138..144
FT                   /evidence="ECO:0007829|PDB:6RIE"
FT   HELIX           152..156
FT                   /evidence="ECO:0007829|PDB:6RIE"
FT   HELIX           163..173
FT                   /evidence="ECO:0007829|PDB:6RIE"
FT   TURN            174..176
FT                   /evidence="ECO:0007829|PDB:6RIE"
FT   STRAND          186..190
FT                   /evidence="ECO:0007829|PDB:6RIE"
FT   HELIX           192..194
FT                   /evidence="ECO:0007829|PDB:6RIE"
FT   STRAND          195..203
FT                   /evidence="ECO:0007829|PDB:6RIE"
FT   HELIX           208..228
FT                   /evidence="ECO:0007829|PDB:6RIE"
FT   STRAND          233..235
FT                   /evidence="ECO:0007829|PDB:6RIE"
FT   STRAND          239..241
FT                   /evidence="ECO:0007829|PDB:6RIE"
FT   STRAND          243..245
FT                   /evidence="ECO:0007829|PDB:6RIE"
FT   TURN            246..249
FT                   /evidence="ECO:0007829|PDB:6RIE"
FT   STRAND          250..253
FT                   /evidence="ECO:0007829|PDB:6RIE"
FT   HELIX           256..264
FT                   /evidence="ECO:0007829|PDB:6RIE"
FT   STRAND          271..276
FT                   /evidence="ECO:0007829|PDB:6RIE"
FT   HELIX           284..302
FT                   /evidence="ECO:0007829|PDB:6RIE"
SQ   SEQUENCE   305 AA;  35393 MW;  702B55EF40A9C0C6 CRC64;
     MQHPREENSI VVELEPSLAT FIKQGFNNLV KWPLLNIGIV LSNTSTAVNE EWLTAVEHIP
     TMKIFYKHIH KILTREMGFL VYLKRSQSER DNYITLYDFD YYIIDKDTNS VTMVDKPTEL
     KETLLHVFQE YRLKSSQTIE LIAFSSGTVI NEDIVSKLTF LDVEVFNREY NNVKTIIDPD
     FVFRSPFIVI SPMGKLTFFV EVYSWFDFKS CFKDIIDFLE GALIANIHNH MIKVGNCDET
     VSSYNPESGM LFVNDLMTMN IVNFFGCNSR LESYHRFDMT KVDVELFIKA LSDACKKILS
     ASNRL