ID   RP35_VACCW              Reviewed;         305 AA.
AC   P24757;
DT   01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-1992, sequence version 1.
DT   03-AUG-2022, entry version 72.
DE   RecName: Full=DNA-directed RNA polymerase 35 kDa subunit;
DE            EC=2.7.7.6;
GN   Name=RPO35; OrderedLocusNames=VACWR152; ORFNames=A29L;
OS   Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS   WR)).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10254;
OH   NCBI_TaxID=9913; Bos taurus (Bovine).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1856205; DOI=10.1016/s0021-9258(18)92757-2;
RA   Amegadzie B.Y., Ahn B.-Y., Moss B.;
RT   "Identification, sequence, and expression of the gene encoding a Mr 35,000
RT   subunit of the vaccinia virus DNA-dependent RNA polymerase.";
RL   J. Biol. Chem. 266:13712-13718(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA   Wohlhueter R.;
RT   "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT   redundancy and an error rate of 0.16/10kb.";
RL   Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   REVIEW.
RX   PubMed=12917449; DOI=10.1099/vir.0.18942-0;
RA   Broyles S.S.;
RT   "Vaccinia virus transcription.";
RL   J. Gen. Virol. 84:2293-2303(2003).
RN   [4]
RP   IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=16509968; DOI=10.1186/1743-422x-3-10;
RA   Yoder J.D., Chen T.S., Gagnier C.R., Vemulapalli S., Maier C.S.,
RA   Hruby D.E.;
RT   "Pox proteomics: mass spectrometry analysis and identification of Vaccinia
RT   virion proteins.";
RL   Virol. J. 3:10-10(2006).
CC   -!- FUNCTION: Part of the DNA-dependent RNA polymerase which catalyzes the
CC       transcription of viral DNA into RNA using the four ribonucleoside
CC       triphosphates as substrates. Responsible for the transcription of
CC       early, intermediate and late genes. DNA-dependent RNA polymerase
CC       associates with the early transcription factor (ETF), itself composed
CC       of D6 and A7, thereby allowing the early genes transcription. Late
CC       transcription, and probably also intermediate transcription, require
CC       newly synthesized RNA polymerase.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6;
CC   -!- SUBUNIT: The DNA-dependent RNA polymerase used for intermediate and
CC       late genes expression consists of eight subunits 147 kDa, 133 kDa, 35
CC       kDa, 30 kDa, 22 kDa, 19 kDa, 18 kDa and 7 kDa totalling more than 500
CC       kDa in mass. The same holoenzyme, with the addition of the
CC       transcription-specificity factor RAP94, is used for early gene
CC       expression.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}. Note=All the enzymes and
CC       other proteins required to synthesize early mRNAs are packaged within
CC       the virion core along with the DNA genome. This is necessary because
CC       viral early mRNAs are synthesized within minutes after virus entry into
CC       the cell and are extruded through pores in the core particle.
CC   -!- SIMILARITY: Belongs to the poxviridae DNA-directed RNA polymerase 35
CC       kDa subunit family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; M61187; AAA48326.1; -; Genomic_DNA.
DR   EMBL; X57318; CAA40579.1; -; Genomic_DNA.
DR   EMBL; AY243312; AAO89431.1; -; Genomic_DNA.
DR   PIR; A39460; RNVZ35.
DR   SMR; P24757; -.
DR   Proteomes; UP000000344; Genome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0019083; P:viral transcription; IEA:InterPro.
DR   InterPro; IPR005059; DNA-dir_RNA_pol_35kDa_poxviral.
DR   Pfam; PF03396; Pox_RNA_pol_35; 1.
DR   PIRSF; PIRSF000746; Rpo35; 1.
PE   1: Evidence at protein level;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transcription; Transferase; Virion.
FT   CHAIN           1..305
FT                   /note="DNA-directed RNA polymerase 35 kDa subunit"
FT                   /id="PRO_0000099134"
SQ   SEQUENCE   305 AA;  35359 MW;  9ECBBBEFA0A36ACC CRC64;
     MQHPREENSI VVELEPSLAT FIKQGFNNLV KWPLLNIGIV LSNTSTAVNE EWLTAVEHIP
     TMKIFYKHIH KILTREMGFL VYLKRSQSER DNYITLYDFD YYIIDKDTNS VTMVDKPTEL
     KETLLHVFQE YRLKSSQTIE LIAFSSGTVI NEDIVSKLTF LDVEVFNREY NNVKTIIDPD
     FVFRSPFIVI SPMGKLTFFV EVYSWFDFKS CLKDIIDFLE GALIANIHNH MIKVGNCDET
     VSSYNPESGM LFVNDLMTMN IVNFFGCNSR LESYHRFDMT KVDVELFIKA LSDACKKILS
     ASNRL