RP54_AZOC5
ID RP54_AZOC5 Reviewed; 514 AA.
AC P33984; A8IKL6;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 26-FEB-2008, sequence version 2.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=RNA polymerase sigma-54 factor;
GN Name=rpoN; Synonyms=ntrA; OrderedLocusNames=AZC_3925;
OS Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / LMG
OS 6465 / NBRC 14845 / NCIMB 13405 / ORS 571).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Xanthobacteraceae; Azorhizobium.
OX NCBI_TaxID=438753;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8471796; DOI=10.1094/mpmi-6-238;
RA Stigter J., Schneider M., de Bruijn F.J.;
RT "Azorhizobium caulinodans nitrogen fixation (nif/fix) gene regulation:
RT mutagenesis of the nifA -24/-12 promoter element, characterization of a
RT ntrA(rpoN) gene, and derivation of a model.";
RL Mol. Plant Microbe Interact. 6:238-252(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43989 / DSM 5975 / JCM 20966 / LMG 6465 / NBRC 14845 / NCIMB
RC 13405 / ORS 571;
RA Lee K.B., Backer P.D., Aono T., Liu C.T., Suzuki S., Suzuki T., Kaneko T.,
RA Yamada M., Tabata S., Kupfer D.M., Najar F.Z., Wiley G.B., Roe B.,
RA Binnewies T., Ussery D., Vereecke D., Gevers D., Holsters M., Oyaizu H.;
RT "Complete genome sequence of the nitrogen-fixing bacterium Azorhizobium
RT caulinodans ORS571.";
RL Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. This sigma factor is responsible for the expression of the
CC nitrogen fixation genes.
CC -!- SIMILARITY: Belongs to the sigma-54 factor family. {ECO:0000305}.
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DR EMBL; X69959; CAA49582.1; -; Genomic_DNA.
DR EMBL; AP009384; BAF89923.1; -; Genomic_DNA.
DR PIR; S33580; S33580.
DR RefSeq; WP_012172445.1; NC_009937.1.
DR AlphaFoldDB; P33984; -.
DR SMR; P33984; -.
DR STRING; 438753.AZC_3925; -.
DR EnsemblBacteria; BAF89923; BAF89923; AZC_3925.
DR KEGG; azc:AZC_3925; -.
DR eggNOG; COG1508; Bacteria.
DR HOGENOM; CLU_020569_0_0_5; -.
DR OMA; VTTQKFM; -.
DR OrthoDB; 1906842at2; -.
DR Proteomes; UP000000270; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0001216; F:DNA-binding transcription activator activity; IEA:InterPro.
DR GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR Gene3D; 1.10.10.1330; -; 1.
DR InterPro; IPR000394; RNA_pol_sigma_54.
DR InterPro; IPR007046; RNA_pol_sigma_54_core-bd.
DR InterPro; IPR007634; RNA_pol_sigma_54_DNA-bd.
DR InterPro; IPR038709; RpoN_core-bd_sf.
DR PANTHER; PTHR32248; PTHR32248; 1.
DR Pfam; PF00309; Sigma54_AID; 1.
DR Pfam; PF04963; Sigma54_CBD; 1.
DR Pfam; PF04552; Sigma54_DBD; 1.
DR PIRSF; PIRSF000774; RpoN; 1.
DR PRINTS; PR00045; SIGMA54FCT.
DR TIGRFAMs; TIGR02395; rpoN_sigma; 1.
DR PROSITE; PS00717; SIGMA54_1; 1.
DR PROSITE; PS00718; SIGMA54_2; 1.
DR PROSITE; PS50044; SIGMA54_3; 1.
PE 3: Inferred from homology;
KW DNA-binding; DNA-directed RNA polymerase; Nucleotidyltransferase;
KW Reference proteome; Sigma factor; Transcription; Transcription regulation;
KW Transferase.
FT CHAIN 1..514
FT /note="RNA polymerase sigma-54 factor"
FT /id="PRO_0000205522"
FT DNA_BIND 391..410
FT /note="H-T-H motif"
FT /evidence="ECO:0000255"
FT REGION 52..91
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 114..133
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 493..514
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 480..488
FT /note="RPON box"
FT CONFLICT 60
FT /note="H -> L (in Ref. 1; CAA49582)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 514 AA; 55888 MW; FEE25CA7530F1481 CRC64;
MAMSPKMEFR QSQSLVMTPQ LMQAIKLLQL SNLELVAYVE AELERNPLLE RASEPESPEH
DPPNPQEEAP TPPDSGAPVS GDWMESDMGS SREAIETRLD TDLGNVFPDD APAERIGAGS
GSGSSIEWGS GGDRGEDYNP EAFLAAETTL ADHLEAQLSV AEPDPARRLI GLNLIGLIDE
TGYFSGDLDA VAEQLGATHD QVADVLRVIQ SFEPSGVGAR SLSECLALQL RDKDRCDPAM
QALLDNLELL ARHDRNALKR ICGVDAEDLA DMIGEIRRLD PKPGLAYGGG VVHPLVPDVF
VREGSDGSWI VELNSETLPR VLVNQTYHAT VAKAARSAEE KTFLADCLQS ASWLTRSLDQ
RARTILKVAS EIVRQQDAFL VHGVRHLRPL NLRTVADAIG MHESTVSRVT SNKYISTPRG
VLEMKFFFSS SIASSGGGEA HAAEAVRHRI KSLIEAESAD DVLSDDTLVQ KLKDDGIDIA
RRTVAKYRES MNIPSSVQRR REKQALRSDA AAAG
