RP54_AZOVI
ID RP54_AZOVI Reviewed; 502 AA.
AC P08623;
DT 01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1988, sequence version 1.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=RNA polymerase sigma-54 factor;
GN Name=rpoN; Synonyms=ntrA;
OS Azotobacter vinelandii.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Azotobacter.
OX NCBI_TaxID=354;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 13705 / OP1 / DSM 366 / NCIMB 11614 / LMG 3878 / UW;
RX PubMed=3481423; DOI=10.1007/bf00325701;
RA Merrick M.J., Gibbins J., Toukdarian A.;
RT "The nucleotide sequence of the sigma factor gene ntrA (rpoN) of
RT Azotobacter vinelandii: analysis of conserved sequences in NtrA proteins.";
RL Mol. Gen. Genet. 210:323-330(1987).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. This sigma factor is responsible for the expression of the
CC nitrogen fixation genes. The open complex (sigma-54 and core RNA
CC polymerase) serves as the receptor for receipt of the melting signal
CC from the remotely bound activator protein NifA for the expression of
CC the nitrogen fixation proteins.
CC -!- SIMILARITY: Belongs to the sigma-54 factor family. {ECO:0000305}.
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DR EMBL; X05888; CAA29314.1; -; Genomic_DNA.
DR PIR; S00720; S00720.
DR RefSeq; WP_041806998.1; NZ_FPKM01000010.1.
DR AlphaFoldDB; P08623; -.
DR SMR; P08623; -.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0001216; F:DNA-binding transcription activator activity; IEA:InterPro.
DR GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR Gene3D; 1.10.10.1330; -; 1.
DR InterPro; IPR000394; RNA_pol_sigma_54.
DR InterPro; IPR007046; RNA_pol_sigma_54_core-bd.
DR InterPro; IPR007634; RNA_pol_sigma_54_DNA-bd.
DR InterPro; IPR038709; RpoN_core-bd_sf.
DR PANTHER; PTHR32248; PTHR32248; 1.
DR Pfam; PF00309; Sigma54_AID; 1.
DR Pfam; PF04963; Sigma54_CBD; 1.
DR Pfam; PF04552; Sigma54_DBD; 1.
DR PIRSF; PIRSF000774; RpoN; 1.
DR PRINTS; PR00045; SIGMA54FCT.
DR TIGRFAMs; TIGR02395; rpoN_sigma; 1.
DR PROSITE; PS00717; SIGMA54_1; 1.
DR PROSITE; PS00718; SIGMA54_2; 1.
DR PROSITE; PS50044; SIGMA54_3; 1.
PE 3: Inferred from homology;
KW DNA-binding; DNA-directed RNA polymerase; Nitrogen fixation;
KW Nucleotidyltransferase; Sigma factor; Transcription;
KW Transcription regulation; Transferase.
FT CHAIN 1..502
FT /note="RNA polymerase sigma-54 factor"
FT /id="PRO_0000205523"
FT DNA_BIND 391..410
FT /note="H-T-H motif"
FT /evidence="ECO:0000255"
FT REGION 40..104
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 479..487
FT /note="RPON box"
FT COMPBIAS 67..81
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 502 AA; 56917 MW; 699A405BE87D4F10 CRC64;
MKPSLVLKMG QQLTMTPQLQ QAIRLLQLST LDLQQEIQEA LDSNPMLERQ EDAEDYDSPD
MLGEHGDQST LDTTPGSYQE GYESGAASED GGTLEEGDWH ERIPSELPVD TAWEDIYQTS
ASNLPSTDED EWDFTTRTST GESLQSHLLW QLNLTPMSDT DRLIAVTLID SINSDGYLEA
ALEEILASLD PELGVELDEV EMVLRRIQQF EPAGIAARDL SESLLLQLRQ LPPDTPWLEE
AKRLAKDYLD LLGNRDFTQL MRRMKLKEEE LRPVIELIQS LNPRPGAQIE SSEPEYVVPD
VIVRKHNDRW LVELNQEAVP RLRINPHYAG FIRRADASAD NTFMRNQLQE ARWFIKSLQS
RNETLMKVST QIVEHQRGFL DYGEEAMKPL VLHDIAEAVG MHESTISRVT TQKYMHTPRG
IYELKYFFSS HVSTAEGGEC SSTAIRAIIK KLIAAENPKK PLSDSKIAGL LEEQGIQVAR
RTVAKYRESL SIAPSSERKR LM
