ID   RP54_BACSU              Reviewed;         436 AA.
AC   P24219;
DT   01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-1992, sequence version 1.
DT   03-AUG-2022, entry version 138.
DE   RecName: Full=RNA polymerase sigma-54 factor;
GN   Name=sigL; OrderedLocusNames=BSU34200;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC   3610 / NRRL NRS-744 / VKM B-501;
RX   PubMed=1924373; DOI=10.1073/pnas.88.20.9092;
RA   Debarbouille M., Martin-Verstraete I., Kunst F., Rapoport G.;
RT   "The Bacillus subtilis sigL gene encodes an equivalent of sigma 54 from
RT   Gram-negative bacteria.";
RL   Proc. Natl. Acad. Sci. U.S.A. 88:9092-9096(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=8969506; DOI=10.1099/13500872-142-11-3089;
RA   Fabret C., Quentin Y., Chapal N., Guiseppi A., Haiech J., Denizot F.;
RT   "Integrated mapping and sequencing of a 115 kb DNA fragment from Bacillus
RT   subtilis: sequence analysis of a 21 kb segment containing the sigL locus.";
RL   Microbiology 142:3089-3096(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RA   Denizot F.;
RL   Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [5]
RP   FUNCTION, SUBUNIT, AND INDUCTION.
RC   STRAIN=168;
RX   PubMed=21710567; DOI=10.1002/pmic.201000790;
RA   Delumeau O., Lecointe F., Muntel J., Guillot A., Guedon E., Monnet V.,
RA   Hecker M., Becher D., Polard P., Noirot P.;
RT   "The dynamic protein partnership of RNA polymerase in Bacillus subtilis.";
RL   Proteomics 11:2992-3001(2011).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase (RNAP) to specific initiation sites and
CC       are then released. This sigma factor is responsible for the expression
CC       of the levanase operon. The open complex (sigma-54 and core RNA
CC       polymerase) serves as the receptor for receipt of the melting signal
CC       from the remotely bound activator protein LevR for the expression of
CC       the levanase operon. Associates with the RNAP core only in stationary
CC       phase cells (PubMed:21710567). {ECO:0000269|PubMed:21710567}.
CC   -!- SUBUNIT: Interacts transiently with the RNAP core.
CC       {ECO:0000305|PubMed:21710567}.
CC   -!- INDUCTION: Association with RNAP core increases slightly during late
CC       sporulation but not tested stresses (at protein level).
CC       {ECO:0000269|PubMed:21710567}.
CC   -!- SIMILARITY: Belongs to the sigma-54 factor family. {ECO:0000305}.
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DR   EMBL; M73443; AAA22753.1; -; Genomic_DNA.
DR   EMBL; Z71928; CAA96485.1; -; Genomic_DNA.
DR   EMBL; Z94043; CAB08001.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB15425.1; -; Genomic_DNA.
DR   PIR; A41229; A41229.
DR   RefSeq; NP_391300.1; NC_000964.3.
DR   RefSeq; WP_003242595.1; NZ_JNCM01000033.1.
DR   AlphaFoldDB; P24219; -.
DR   SMR; P24219; -.
DR   STRING; 224308.BSU34200; -.
DR   PaxDb; P24219; -.
DR   PRIDE; P24219; -.
DR   DNASU; 936362; -.
DR   EnsemblBacteria; CAB15425; CAB15425; BSU_34200.
DR   GeneID; 936362; -.
DR   KEGG; bsu:BSU34200; -.
DR   PATRIC; fig|224308.179.peg.3707; -.
DR   eggNOG; COG1508; Bacteria.
DR   InParanoid; P24219; -.
DR   OMA; VTTQKFM; -.
DR   PhylomeDB; P24219; -.
DR   BioCyc; BSUB:BSU34200-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0001216; F:DNA-binding transcription activator activity; IEA:InterPro.
DR   GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR   Gene3D; 1.10.10.1330; -; 1.
DR   InterPro; IPR000394; RNA_pol_sigma_54.
DR   InterPro; IPR007046; RNA_pol_sigma_54_core-bd.
DR   InterPro; IPR007634; RNA_pol_sigma_54_DNA-bd.
DR   InterPro; IPR038709; RpoN_core-bd_sf.
DR   PANTHER; PTHR32248; PTHR32248; 1.
DR   Pfam; PF00309; Sigma54_AID; 1.
DR   Pfam; PF04963; Sigma54_CBD; 1.
DR   Pfam; PF04552; Sigma54_DBD; 1.
DR   PIRSF; PIRSF000774; RpoN; 1.
DR   PRINTS; PR00045; SIGMA54FCT.
DR   TIGRFAMs; TIGR02395; rpoN_sigma; 1.
DR   PROSITE; PS00717; SIGMA54_1; 1.
DR   PROSITE; PS00718; SIGMA54_2; 1.
DR   PROSITE; PS50044; SIGMA54_3; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; DNA-directed RNA polymerase; Nucleotidyltransferase;
KW   Reference proteome; Sigma factor; Transcription; Transcription regulation;
KW   Transferase.
FT   CHAIN           1..436
FT                   /note="RNA polymerase sigma-54 factor"
FT                   /id="PRO_0000205526"
FT   DNA_BIND        324..343
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   MOTIF           413..421
FT                   /note="RPON box"
SQ   SEQUENCE   436 AA;  49701 MW;  48C60D4BD306BF24 CRC64;
     MDMKLQQVQV LKPQLTQELR QAITLLGYHS AELAEYIDEL SLENPLIERK ETDTPPLSYH
     KTNKNRMNAQ EAGLQLSNPQ KTLQDALKQQ SLDMNLTNTE KKIFNYLIHS LDSNGYLEED
     IEEAARRLSV SAKEAEAVLA KLQSLEPAGI GARSLQECIL LQLQRLPNRN EQAEMLVSAH
     FDAFAQKKWK TLSVETGIPL HTIQDISDDI AALHPRPGLL FARPEQDVYI EPDIFITVKN
     GHIAAELNTR SFPEIDLHPQ YRTLLSSGSC QDTVSYLSAK YQEWRWLSRA LRQRKQTITR
     IINELITRQK DFFLKGRSAM KPLTLREVAD CLSLHESTVS RAIKGKTIQT PYGLFEMKLF
     FSAKAEASGD GDASNYAVKT HLENLINQED KTKPLSDQKL VDLLYEQHGI QISRRTVAKY
     RDQMNIPSSA ARKRYK