ID   RP54_BRADU              Reviewed;         484 AA.
AC   P30332;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1993, sequence version 1.
DT   03-AUG-2022, entry version 130.
DE   RecName: Full=RNA polymerase sigma-54 factor 1;
GN   Name=rpoN1; OrderedLocusNames=blr1883;
OS   Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 /
OS   NBRC 14792 / USDA 110).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Bradyrhizobiaceae; Bradyrhizobium.
OX   NCBI_TaxID=224911;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=USDA 110spc4;
RX   PubMed=1991712; DOI=10.1128/jb.173.3.1125-1138.1991;
RA   Kullik I., Fritsche S., Knobel H., Sanjuan J., Hennecke H., Fischer H.-M.;
RT   "Bradyrhizobium japonicum has two differentially regulated, functional
RT   homologs of the sigma 54 gene (rpoN).";
RL   J. Bacteriol. 173:1125-1138(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX   PubMed=12597275; DOI=10.1093/dnares/9.6.189;
RA   Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S.,
RA   Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M.,
RA   Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.;
RT   "Complete genomic sequence of nitrogen-fixing symbiotic bacterium
RT   Bradyrhizobium japonicum USDA110.";
RL   DNA Res. 9:189-197(2002).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. This sigma factor is responsible for the expression of the
CC       nitrogen fixation genes.
CC   -!- INDUCTION: Regulated in response to oxygen.
CC   -!- SIMILARITY: Belongs to the sigma-54 factor family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; M59242; AAA26240.1; -; Genomic_DNA.
DR   EMBL; BA000040; BAC47148.1; -; Genomic_DNA.
DR   PIR; A38179; A38179.
DR   RefSeq; NP_768523.1; NC_004463.1.
DR   RefSeq; WP_011084688.1; NZ_CP011360.1.
DR   AlphaFoldDB; P30332; -.
DR   SMR; P30332; -.
DR   STRING; 224911.27350136; -.
DR   EnsemblBacteria; BAC47148; BAC47148; BAC47148.
DR   GeneID; 64067156; -.
DR   KEGG; bja:blr1883; -.
DR   PATRIC; fig|224911.44.peg.1362; -.
DR   eggNOG; COG1508; Bacteria.
DR   HOGENOM; CLU_020569_0_0_5; -.
DR   InParanoid; P30332; -.
DR   OMA; NPPAIEF; -.
DR   PhylomeDB; P30332; -.
DR   Proteomes; UP000002526; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0001216; F:DNA-binding transcription activator activity; IEA:InterPro.
DR   GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR   GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.1330; -; 1.
DR   InterPro; IPR000394; RNA_pol_sigma_54.
DR   InterPro; IPR007046; RNA_pol_sigma_54_core-bd.
DR   InterPro; IPR007634; RNA_pol_sigma_54_DNA-bd.
DR   InterPro; IPR038709; RpoN_core-bd_sf.
DR   PANTHER; PTHR32248; PTHR32248; 1.
DR   Pfam; PF00309; Sigma54_AID; 1.
DR   Pfam; PF04963; Sigma54_CBD; 1.
DR   Pfam; PF04552; Sigma54_DBD; 1.
DR   PIRSF; PIRSF000774; RpoN; 1.
DR   PRINTS; PR00045; SIGMA54FCT.
DR   TIGRFAMs; TIGR02395; rpoN_sigma; 1.
DR   PROSITE; PS00717; SIGMA54_1; 1.
DR   PROSITE; PS00718; SIGMA54_2; 1.
DR   PROSITE; PS50044; SIGMA54_3; 1.
PE   2: Evidence at transcript level;
KW   DNA-binding; DNA-directed RNA polymerase; Nitrogen fixation;
KW   Nucleotidyltransferase; Reference proteome; Sigma factor; Transcription;
KW   Transcription regulation; Transferase.
FT   CHAIN           1..484
FT                   /note="RNA polymerase sigma-54 factor 1"
FT                   /id="PRO_0000205524"
FT   DNA_BIND        355..374
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255"
FT   REGION          464..484
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           444..452
FT                   /note="RPON box"
FT   COMPBIAS        464..478
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   484 AA;  53773 MW;  BA5B66FB218D268A CRC64;
     MALTQRLEFR QSQSLVMSPQ LMQAIKLLQL SNLDLMTFVE EELECNPLLE RASDDAAGAE
     APTEVDQVSG DQLAEAQVRD ARDGAMTTYT EWGGGGSGDE DYNLEAFVAS ETTLSDHLAE
     QLSVAFTAPA QRMIGQYLID LVDEAGYLPP DLGQAAERLG ATQEDVEHVL AVLQEFDPPG
     VCARNLRECL AIQLRELDRY DPAMQALVEH LDLLAKRDIA SLRKLCGVDD EDIADMIDEL
     RRLSPKPGMK FGSARLQTMV PDVYVRPAPD GGWHVELNSD TLPRVLVNQT YYSKLSKKIG
     KDVDKSYFND ALQNATWLVR ALDQRARTIL KVATEIVRQQ DGFFTLGVAH LRPLNLKAVA
     EAIQMHESTV SRVTANKYMA TNRGTFELKY FFTASIPSAD GGEAHSAEAV RHRIKQLIES
     EEPSAVLSDD AIVERLRVSG IDIARRTVAK YREAMRIRSS VQRRRDNMWS TMNSRASGGT
     GLDK