RP54_CAUVC
ID RP54_CAUVC Reviewed; 497 AA.
AC Q03408; O08456; O32343;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 27-APR-2001, sequence version 3.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=RNA polymerase sigma-54 factor;
GN Name=rpoN; Synonyms=flbU; OrderedLocusNames=CC_3599;
OS Caulobacter vibrioides (strain ATCC 19089 / CB15) (Caulobacter crescentus).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Caulobacterales;
OC Caulobacteraceae; Caulobacter.
OX NCBI_TaxID=190650;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 19089 / CB15;
RX PubMed=1459461; DOI=10.1101/gad.6.12a.2395;
RA Brun Y.V., Shapiro L.;
RT "A temporally controlled sigma-factor is required for polar morphogenesis
RT and normal cell division in Caulobacter.";
RL Genes Dev. 6:2395-2408(1992).
RN [2]
RP SEQUENCE REVISION.
RA Janakiraman R.S.;
RL Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 19089 / CB15;
RX PubMed=7898437; DOI=10.1007/bf00290715;
RA Anderson D.K., Ohta N., Wu J., Newton A.;
RT "Regulation of the Caulobacter crescentus rpoN gene and function of the
RT purified sigma 54 in flagellar gene transcription.";
RL Mol. Gen. Genet. 246:697-706(1995).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=9260957; DOI=10.1128/jb.179.16.5138-5147.1997;
RA Janakiraman R.S., Brun Y.V.;
RT "Transcriptional and mutational analyses of the rpoN operon in Caulobacter
RT crescentus.";
RL J. Bacteriol. 179:5138-5147(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 19089 / CB15;
RX PubMed=11259647; DOI=10.1073/pnas.061029298;
RA Nierman W.C., Feldblyum T.V., Laub M.T., Paulsen I.T., Nelson K.E.,
RA Eisen J.A., Heidelberg J.F., Alley M.R.K., Ohta N., Maddock J.R.,
RA Potocka I., Nelson W.C., Newton A., Stephens C., Phadke N.D., Ely B.,
RA DeBoy R.T., Dodson R.J., Durkin A.S., Gwinn M.L., Haft D.H., Kolonay J.F.,
RA Smit J., Craven M.B., Khouri H.M., Shetty J., Berry K.J., Utterback T.R.,
RA Tran K., Wolf A.M., Vamathevan J.J., Ermolaeva M.D., White O.,
RA Salzberg S.L., Venter J.C., Shapiro L., Fraser C.M.;
RT "Complete genome sequence of Caulobacter crescentus.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:4136-4141(2001).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. This sigma factor is responsible for the expression of the
CC nitrogen fixation genes. This sigma factor is required for the de novo
CC biogenesis of both flagellum and stalk, and for polar morphogenesis and
CC normal cell division. It may not be involved in nitrogen assimilation.
CC -!- SIMILARITY: Belongs to the sigma-54 factor family. {ECO:0000305}.
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DR EMBL; X68549; CAA48553.1; -; Genomic_DNA.
DR EMBL; L11003; AAA73956.1; -; Genomic_DNA.
DR EMBL; AF006743; AAC45594.1; -; Genomic_DNA.
DR EMBL; AE005673; AAK25561.1; -; Genomic_DNA.
DR PIR; S54743; I40668.
DR RefSeq; NP_422393.1; NC_002696.2.
DR RefSeq; WP_010921426.1; NC_002696.2.
DR AlphaFoldDB; Q03408; -.
DR SMR; Q03408; -.
DR STRING; 190650.CC_3599; -.
DR EnsemblBacteria; AAK25561; AAK25561; CC_3599.
DR KEGG; ccr:CC_3599; -.
DR PATRIC; fig|190650.5.peg.3602; -.
DR eggNOG; COG1508; Bacteria.
DR HOGENOM; CLU_020569_0_0_5; -.
DR OMA; VTTQKFM; -.
DR BioCyc; CAULO:CC3599-MON; -.
DR Proteomes; UP000001816; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0001216; F:DNA-binding transcription activator activity; IEA:InterPro.
DR GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR Gene3D; 1.10.10.1330; -; 1.
DR InterPro; IPR000394; RNA_pol_sigma_54.
DR InterPro; IPR007046; RNA_pol_sigma_54_core-bd.
DR InterPro; IPR007634; RNA_pol_sigma_54_DNA-bd.
DR InterPro; IPR038709; RpoN_core-bd_sf.
DR PANTHER; PTHR32248; PTHR32248; 1.
DR Pfam; PF00309; Sigma54_AID; 1.
DR Pfam; PF04963; Sigma54_CBD; 1.
DR Pfam; PF04552; Sigma54_DBD; 1.
DR PIRSF; PIRSF000774; RpoN; 1.
DR PRINTS; PR00045; SIGMA54FCT.
DR TIGRFAMs; TIGR02395; rpoN_sigma; 1.
DR PROSITE; PS00717; SIGMA54_1; 1.
DR PROSITE; PS00718; SIGMA54_2; 1.
DR PROSITE; PS50044; SIGMA54_3; 1.
PE 3: Inferred from homology;
KW DNA-binding; DNA-directed RNA polymerase; Nucleotidyltransferase;
KW Reference proteome; Sigma factor; Transcription; Transcription regulation;
KW Transferase.
FT CHAIN 1..497
FT /note="RNA polymerase sigma-54 factor"
FT /id="PRO_0000205527"
FT DNA_BIND 380..399
FT /note="H-T-H motif"
FT /evidence="ECO:0000250"
FT REGION 49..128
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 471..479
FT /note="RPON box"
FT COMPBIAS 49..68
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 26
FT /note="K -> M (in Ref. 1 and 4)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 497 AA; 54457 MW; 2F877D258FB984B8 CRC64;
MALSHRLELR QGQGLVITPQ LQQAIKLLQL SNIELDAFVE AELERNPLLQ RDEGDHEPAV
EVEAERDASL TQVDAVADTT AGRELDTPVD DVSPGERATG EGTDAEHAGG QIDWSRAGGG
GSFESDEGYE RALTDSPTLA AHLRTQLVQA ALSPAHNAIA EILIDAVDEG GYLRLDIVEL
ADRLGCALAL VEETLSVLQG FEPVGVFARD VRECLALQLK DLNRYDPAMA AMLDHLELLA
KRDMAGLRRI CGVDDEDLRE MIGEIRSLNP RPGAAYHSEP AETLVPDVMV REGLGGMWHV
ELNTDTLPRV LVDQRYHARV SKGARSDQEK TFVADCMASA NWLVKSLDQR AKTILKVASE
IVRQQDGFLA FGVEHLRPLN LKTVADAIGM HESTVSRVTS NKYIATPRGV FELKFFFTSA
IQSSEGGEAH SAASVRHKIK GLVDAEKCEA DVHSDDRIVE ILKAAGVDIA RRTVAKYREA
MRIPSSVERR RLMKEAV
