ID   RP54_CLOK5              Reviewed;         453 AA.
AC   P38944; A5N1M5;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   26-FEB-2008, sequence version 3.
DT   03-AUG-2022, entry version 109.
DE   RecName: Full=Putative RNA polymerase sigma-54 factor;
GN   Name=rpoN; Synonyms=sigL; OrderedLocusNames=CKL_3013;
OS   Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=431943;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8527 / DSM 555 / NCIMB 10680;
RX   PubMed=18218779; DOI=10.1073/pnas.0711093105;
RA   Seedorf H., Fricke W.F., Veith B., Brueggemann H., Liesegang H.,
RA   Strittmatter A., Miethke M., Buckel W., Hinderberger J., Li F.,
RA   Hagemeier C., Thauer R.K., Gottschalk G.;
RT   "The genome of Clostridium kluyveri, a strict anaerobe with unique
RT   metabolic features.";
RL   Proc. Natl. Acad. Sci. U.S.A. 105:2128-2133(2008).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-29.
RX   PubMed=8550525; DOI=10.1128/jb.178.3.871-880.1996;
RA   Soehling B., Gottschalk G.;
RT   "Molecular analysis of the anaerobic succinate degradation pathway in
RT   Clostridium kluyveri.";
RL   J. Bacteriol. 178:871-880(1996).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released.
CC   -!- SIMILARITY: Belongs to the sigma-54 factor family. {ECO:0000305}.
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DR   EMBL; CP000673; EDK35021.1; -; Genomic_DNA.
DR   EMBL; L21902; AAA92349.1; -; Genomic_DNA.
DR   AlphaFoldDB; P38944; -.
DR   SMR; P38944; -.
DR   STRING; 431943.CKL_3013; -.
DR   EnsemblBacteria; EDK35021; EDK35021; CKL_3013.
DR   KEGG; ckl:CKL_3013; -.
DR   eggNOG; COG1508; Bacteria.
DR   HOGENOM; CLU_020569_1_1_9; -.
DR   OMA; NPPAIEF; -.
DR   Proteomes; UP000002411; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0001216; F:DNA-binding transcription activator activity; IEA:InterPro.
DR   GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR   Gene3D; 1.10.10.1330; -; 1.
DR   InterPro; IPR000394; RNA_pol_sigma_54.
DR   InterPro; IPR007046; RNA_pol_sigma_54_core-bd.
DR   InterPro; IPR007634; RNA_pol_sigma_54_DNA-bd.
DR   InterPro; IPR038709; RpoN_core-bd_sf.
DR   PANTHER; PTHR32248; PTHR32248; 1.
DR   Pfam; PF00309; Sigma54_AID; 1.
DR   Pfam; PF04963; Sigma54_CBD; 1.
DR   Pfam; PF04552; Sigma54_DBD; 1.
DR   PIRSF; PIRSF000774; RpoN; 1.
DR   PRINTS; PR00045; SIGMA54FCT.
DR   TIGRFAMs; TIGR02395; rpoN_sigma; 1.
DR   PROSITE; PS00717; SIGMA54_1; 1.
DR   PROSITE; PS00718; SIGMA54_2; 1.
DR   PROSITE; PS50044; SIGMA54_3; 1.
PE   3: Inferred from homology;
KW   DNA-binding; DNA-directed RNA polymerase; Nucleotidyltransferase;
KW   Reference proteome; Sigma factor; Transcription; Transcription regulation;
KW   Transferase.
FT   CHAIN           1..453
FT                   /note="Putative RNA polymerase sigma-54 factor"
FT                   /id="PRO_0000205528"
FT   DNA_BIND        342..361
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   MOTIF           431..439
FT                   /note="RPON box"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   453 AA;  53029 MW;  39D5B2720B524504 CRC64;
     MDFALNLTQE QRLAMTQEMQ LSIKLLQMSS FELQEYVEKE LQENPVLDIK ESNVDKVEKD
     KLEYKEIIKN LDFDNYSHHS YDRNPDEEVS PFNFISEEKS LKEYLKDQIR DLDENQCIKT
     ICLYIIENID DRGYLILENE EIEEELKISK ELAAYCVDLI HSLEPHGIGA RNLAECLKIQ
     IKQKDMDEEN IFIMIDKYLE FIAENKYNVI AKELNIDVKQ AQEYGDLIKT LSPKPSRGFY
     TGESVRYISP DAYIKKIDSE YFIIMNDDLT PRLTINAIYK DIINNDTDKD AVSYVKEKLN
     SALFLIKSIQ HRKSTIYRVL EKIVEIQKDY FDYGESYLKP MTLKEIASSM DMHESTVSRA
     IRDKYIHINR GIVKIKDLFT TGMAASFSEE NVSSLIIKNS IKKMIDNEDR KKPLSDQKIC
     DLINEEGMNI SRRTVAKYRE EMGIKSSKGR KRF