ID   RP54_CUPNH              Reviewed;         493 AA.
AC   P28615; Q0KEN4;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   03-AUG-2022, entry version 134.
DE   RecName: Full=RNA polymerase sigma-54 factor;
GN   Name=rpoN; Synonyms=hno, ntrA; OrderedLocusNames=H16_A0387;
OS   Cupriavidus necator (strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442
OS   / H16 / Stanier 337) (Ralstonia eutropha).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Cupriavidus.
OX   NCBI_TaxID=381666;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1417413; DOI=10.1007/bf00245213;
RA   Warrelmann J., Eitinger M., Schwartz E., Rermann D., Friedrich B.;
RT   "Nucleotide sequence of the rpoN (hno) gene region of Alcaligenes
RT   eutrophus: evidence for a conserved gene cluster.";
RL   Arch. Microbiol. 158:107-114(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442 / H16 / Stanier 337;
RX   PubMed=16964242; DOI=10.1038/nbt1244;
RA   Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R.,
RA   Eitinger T., Ewering C., Poetter M., Schwartz E., Strittmatter A., Voss I.,
RA   Gottschalk G., Steinbuechel A., Friedrich B., Bowien B.;
RT   "Genome sequence of the bioplastic-producing 'Knallgas' bacterium Ralstonia
RT   eutropha H16.";
RL   Nat. Biotechnol. 24:1257-1262(2006).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released.
CC   -!- SIMILARITY: Belongs to the sigma-54 factor family. {ECO:0000305}.
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DR   EMBL; X66793; CAA47287.1; -; Genomic_DNA.
DR   EMBL; AM260479; CAJ91537.1; -; Genomic_DNA.
DR   PIR; B48362; B48362.
DR   RefSeq; WP_011614486.1; NZ_CP039287.1.
DR   AlphaFoldDB; P28615; -.
DR   SMR; P28615; -.
DR   STRING; 381666.H16_A0387; -.
DR   EnsemblBacteria; CAJ91537; CAJ91537; H16_A0387.
DR   GeneID; 57642482; -.
DR   KEGG; reh:H16_A0387; -.
DR   PATRIC; fig|381666.6.peg.750; -.
DR   eggNOG; COG1508; Bacteria.
DR   HOGENOM; CLU_020569_0_1_4; -.
DR   OMA; VTTQKFM; -.
DR   OrthoDB; 1906842at2; -.
DR   Proteomes; UP000008210; Chromosome 1.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0001216; F:DNA-binding transcription activator activity; IEA:InterPro.
DR   GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR   Gene3D; 1.10.10.1330; -; 1.
DR   InterPro; IPR000394; RNA_pol_sigma_54.
DR   InterPro; IPR007046; RNA_pol_sigma_54_core-bd.
DR   InterPro; IPR007634; RNA_pol_sigma_54_DNA-bd.
DR   InterPro; IPR038709; RpoN_core-bd_sf.
DR   PANTHER; PTHR32248; PTHR32248; 1.
DR   Pfam; PF00309; Sigma54_AID; 1.
DR   Pfam; PF04963; Sigma54_CBD; 1.
DR   Pfam; PF04552; Sigma54_DBD; 1.
DR   PIRSF; PIRSF000774; RpoN; 1.
DR   PRINTS; PR00045; SIGMA54FCT.
DR   TIGRFAMs; TIGR02395; rpoN_sigma; 1.
DR   PROSITE; PS00717; SIGMA54_1; 1.
DR   PROSITE; PS00718; SIGMA54_2; 1.
DR   PROSITE; PS50044; SIGMA54_3; 1.
PE   3: Inferred from homology;
KW   DNA-binding; DNA-directed RNA polymerase; Nucleotidyltransferase;
KW   Reference proteome; Sigma factor; Transcription; Transcription regulation;
KW   Transferase.
FT   CHAIN           1..493
FT                   /note="RNA polymerase sigma-54 factor"
FT                   /id="PRO_0000205521"
FT   DNA_BIND        383..402
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255"
FT   REGION          68..131
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           471..479
FT                   /note="RPON box"
FT   COMPBIAS        115..131
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        6..7
FT                   /note="QL -> HV (in Ref. 1; CAA47287)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   493 AA;  54770 MW;  6EC120BEF1B0F9EA CRC64;
     MKPSLQLRLS QHLALTPQLQ QSIRLLQLST LELQQEVEQA LTENPLLERE NDWIESPLRV
     AADGSVNLQS APAPAPAEPQ GNGEARADGA ADDDSYGDSG NGDDYGSSDW SLDDFARRPQ
     GDEDEKTPMQ LREAEPTLRE YLMEQLTPLK ISARDKGLAI FLIESLDDDG YLSASLEEIC
     TELPEELEFE IEEVHAILTL LQSFDPPGVG ARNAAECLAL QLRRLTHPQR ELALNIVTNH
     LELLAVRDYT RLKKALQVDE AALKSAHELI RSLAPYPGHA YSRPEADFVV PDVFVRKGGG
     GWIAQLNPDV MPRLRINDMY AQILRGAKGE SGTAGLQQKL QEARWLIKNI QQRFDKILRV
     SQAIVERQKN FFSHGEIAMR PLVLREIADT LGLHESTISR VTTNKYMATP MGTFELKYFF
     GSHVSTETGG AASSTAIRAL IKQLIGAEDP RNPLSDSRIA ELLGEQGFVV ARRTVAKYRE
     ALKIPAVNLR KSL