ID   RP54_PSEAB              Reviewed;         497 AA.
AC   A0A0H2ZGR9;
DT   12-AUG-2020, integrated into UniProtKB/Swiss-Prot.
DT   16-SEP-2015, sequence version 1.
DT   03-AUG-2022, entry version 40.
DE   RecName: Full=RNA polymerase sigma-54 factor;
GN   Name=rpoN; OrderedLocusNames=PA14_57940;
OS   Pseudomonas aeruginosa (strain UCBPP-PA14).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208963;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=UCBPP-PA14;
RX   PubMed=17038190; DOI=10.1186/gb-2006-7-10-r90;
RA   Lee D.G., Urbach J.M., Wu G., Liberati N.T., Feinbaum R.L., Miyata S.,
RA   Diggins L.T., He J., Saucier M., Deziel E., Friedman L., Li L., Grills G.,
RA   Montgomery K., Kucherlapati R., Rahme L.G., Ausubel F.M.;
RT   "Genomic analysis reveals that Pseudomonas aeruginosa virulence is
RT   combinatorial.";
RL   Genome Biol. 7:R90.1-R90.14(2006).
RN   [2]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=11717271; DOI=10.1128/jb.183.24.7126-7134.2001;
RA   Hendrickson E.L., Plotnikova J., Mahajan-Miklos S., Rahme L.G.,
RA   Ausubel F.M.;
RT   "Differential roles of the Pseudomonas aeruginosa PA14 rpoN gene in
RT   pathogenicity in plants, nematodes, insects, and mice.";
RL   J. Bacteriol. 183:7126-7134(2001).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. Plays a role in the regulation of many virulence factors,
CC       motility, quorum sensing, mucoidy, a general mechanism for maintaining
CC       lytic phage in populations of bacteria, and biofilm formation.
CC       Positively controls the T6 secretion system by directly binding to the
CC       promoter regions of hcpA and hcpB, leading to their expression (By
CC       similarity). Thereby, allows to colonize several hosts efficiently
CC       including mammals, insects, nematodes and plants (PubMed:11717271).
CC       {ECO:0000250|UniProtKB:P49988, ECO:0000269|PubMed:11717271}.
CC   -!- DISRUPTION PHENOTYPE: Mutants exhibit reduced pathogenicity in mice, C.
CC       elegans as well as Arabiposis. {ECO:0000269|PubMed:11717271}.
CC   -!- SIMILARITY: Belongs to the sigma-54 factor family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CP000438; ABJ13730.1; -; Genomic_DNA.
DR   RefSeq; WP_003094357.1; NZ_CP034244.1.
DR   AlphaFoldDB; A0A0H2ZGR9; -.
DR   SMR; A0A0H2ZGR9; -.
DR   EnsemblBacteria; ABJ13730; ABJ13730; PA14_57940.
DR   KEGG; pau:PA14_57940; -.
DR   HOGENOM; CLU_020569_0_1_6; -.
DR   OMA; VTTQKFM; -.
DR   BioCyc; PAER208963:G1G74-4880-MON; -.
DR   Proteomes; UP000000653; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0001216; F:DNA-binding transcription activator activity; IEA:InterPro.
DR   GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR   Gene3D; 1.10.10.1330; -; 1.
DR   InterPro; IPR000394; RNA_pol_sigma_54.
DR   InterPro; IPR007046; RNA_pol_sigma_54_core-bd.
DR   InterPro; IPR007634; RNA_pol_sigma_54_DNA-bd.
DR   InterPro; IPR038709; RpoN_core-bd_sf.
DR   PANTHER; PTHR32248; PTHR32248; 1.
DR   Pfam; PF00309; Sigma54_AID; 1.
DR   Pfam; PF04963; Sigma54_CBD; 1.
DR   Pfam; PF04552; Sigma54_DBD; 1.
DR   PIRSF; PIRSF000774; RpoN; 1.
DR   PRINTS; PR00045; SIGMA54FCT.
DR   TIGRFAMs; TIGR02395; rpoN_sigma; 1.
DR   PROSITE; PS00717; SIGMA54_1; 1.
DR   PROSITE; PS00718; SIGMA54_2; 1.
PE   3: Inferred from homology;
KW   DNA-binding; DNA-directed RNA polymerase; Nucleotidyltransferase;
KW   Sigma factor; Transcription; Transcription regulation; Transferase.
FT   CHAIN           1..497
FT                   /note="RNA polymerase sigma-54 factor"
FT                   /id="PRO_0000450718"
FT   DNA_BIND        386..405
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255"
FT   REGION          42..100
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           474..482
FT                   /note="RPON box"
FT                   /evidence="ECO:0000250|UniProtKB:P49988"
FT   COMPBIAS        72..89
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   497 AA;  56054 MW;  CE1994F03FD58405 CRC64;
     MKPSLVLKMG QQLTMTPQLQ QAIRLLQLST LDLQQEIQEA LESNPMLERQ EDGDDFDNSD
     PLADGAEQAA SAPQESPLQE SATPSVESLD DDQWSERIPS ELPVDTAWED IYQTSASSLP
     SNDDDEWDFT ARTSSGESLH SHLLWQVNLA PMSDTDRMIA VTIIDSINND GYLEESLEEI
     LAAIDPELDV ELDEVEVVLR RIQQLEPAGI GARNLRECLL LQLRQLPSTT PWLNEALRLV
     SDYLDLLGGR DYSQLMRRMK LKEDELRQVI ELIQCLHPRP GSQIESSEAE YIVPDVIVRK
     DNERWLVELN QEAMPRLRVN ATYAGMVRRA DSSADNTFMR NQLQEARWFI KSLQSRNETL
     MKVATQIVEH QRGFLDYGEE AMKPLVLHDI AEAVGMHEST ISRVTTQKYM HTPRGIFELK
     YFFSSHVSTA EGGECSSTAI RAIIKKLVAA ENAKKPLSDS KIAGLLEAQG IQVARRTVAK
     YRESLGIAPS SERKRLV