RP54_PSEAE
ID RP54_PSEAE Reviewed; 497 AA.
AC P49988;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 08-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=RNA polymerase sigma-54 factor;
GN Name=rpoN; OrderedLocusNames=PA4462;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=PAK;
RX PubMed=8113171; DOI=10.1128/jb.176.5.1316-1322.1994;
RA Jin S., Ishimoto K., Lory S.;
RT "Nucleotide sequence of the rpoN gene and characterization of two
RT downstream open reading frames in Pseudomonas aeruginosa.";
RL J. Bacteriol. 176:1316-1322(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
RN [3]
RP FUNCTION IN MOTILITY, AND DISRUPTION PHENOTYPE.
RC STRAIN=PAK;
RX PubMed=2152909; DOI=10.1128/jb.172.1.389-396.1990;
RA Totten P.A., Lara J.C., Lory S.;
RT "The rpoN gene product of Pseudomonas aeruginosa is required for expression
RT of diverse genes, including the flagellin gene.";
RL J. Bacteriol. 172:389-396(1990).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=9595661; DOI=10.1111/j.1574-6968.1998.tb12975.x;
RA Yamano Y., Nishikawa T., Komatsu Y.;
RT "Involvement of the RpoN protein in the transcription of the oprE gene in
RT Pseudomonas aeruginosa.";
RL FEMS Microbiol. Lett. 162:31-37(1998).
RN [5]
RP FUNCTION IN QUORUM SENSING.
RX PubMed=12644493; DOI=10.1128/jb.185.7.2227-2235.2003;
RA Heurlier K., Denervaud V., Pessi G., Reimmann C., Haas D.;
RT "Negative control of quorum sensing by RpoN (sigma54) in Pseudomonas
RT aeruginosa PAO1.";
RL J. Bacteriol. 185:2227-2235(2003).
RN [6]
RP FUNCTION IN MUCOIDY.
RX PubMed=10792721; DOI=10.1046/j.1365-2958.2000.01846.x;
RA Boucher J.C., Schurr M.J., Deretic V.;
RT "Dual regulation of mucoidy in Pseudomonas aeruginosa and sigma factor
RT antagonism.";
RL Mol. Microbiol. 36:341-351(2000).
RN [7]
RP FUNCTION IN QUORUM SENSING.
RX PubMed=26633362; DOI=10.3390/ijms161226103;
RA Cai Z., Liu Y., Chen Y., Yam J.K., Chew S.C., Chua S.L., Wang K.,
RA Givskov M., Yang L.;
RT "RpoN Regulates Virulence Factors of Pseudomonas aeruginosa via Modulating
RT the PqsR Quorum Sensing Regulator.";
RL Int. J. Mol. Sci. 16:28311-28319(2015).
RN [8]
RP FUNCTION IN QUORUM SENSING AND BIOFILM FORMATION, AND DISRUPTION PHENOTYPE.
RX PubMed=29760208; DOI=10.1128/jb.00205-18;
RA Shao X., Zhang X., Zhang Y., Zhu M., Yang P., Yuan J., Xie Y., Zhou T.,
RA Wang W., Chen S., Liang H., Deng X.;
RT "RpoN-Dependent Direct Regulation of Quorum Sensing and the Type VI
RT Secretion System in Pseudomonas aeruginosa PAO1.";
RL J. Bacteriol. 200:0-0(2018).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released (PubMed:9595661). Plays a role in the regulation of many
CC virulence factors, motility, quorum sensing, mucoidy, a general
CC mechanism for maintaining lytic phage in populations of bacteria, and
CC biofilm formation (PubMed:2152909, PubMed:12644493, PubMed:10792721,
CC PubMed:26633362, PubMed:29760208). Positively controls the T6 secretion
CC system by directly binding to the promoter regions of hcpA and hcpB
CC genes, leading to their expression (PubMed:29760208). Thereby, allows
CC to colonize several hosts efficiently including mammals, insects,
CC nematodes and plants (By similarity) (PubMed:2152909, PubMed:9595661,
CC PubMed:12644493, PubMed:10792721, PubMed:26633362, PubMed:29760208).
CC {ECO:0000250|UniProtKB:A0A0H2ZGR9, ECO:0000269|PubMed:10792721,
CC ECO:0000269|PubMed:12644493, ECO:0000269|PubMed:2152909,
CC ECO:0000269|PubMed:26633362, ECO:0000269|PubMed:29760208,
CC ECO:0000269|PubMed:9595661}.
CC -!- DISRUPTION PHENOTYPE: Deletion mutants result in reduced production of
CC pyocyanine, motility, and proteolytic activity. The motility defect is
CC due to the inability of these mutants to synthesize flagellin
CC (PubMed:2152909). Significantly decreased porin oprE expression under
CC aerobic conditions (PubMed:9595661). Mutants also show defect in
CC biofilm formation (PubMed:29760208). {ECO:0000269|PubMed:2152909,
CC ECO:0000269|PubMed:29760208, ECO:0000269|PubMed:9595661}.
CC -!- SIMILARITY: Belongs to the sigma-54 factor family. {ECO:0000305}.
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DR EMBL; L26916; AAA19793.1; -; Unassigned_DNA.
DR EMBL; AE004091; AAG07850.1; -; Genomic_DNA.
DR PIR; A53373; A53373.
DR PIR; F83087; F83087.
DR RefSeq; NP_253152.1; NC_002516.2.
DR RefSeq; WP_003094357.1; NZ_QZGE01000004.1.
DR AlphaFoldDB; P49988; -.
DR SMR; P49988; -.
DR MINT; P49988; -.
DR STRING; 287.DR97_1641; -.
DR PaxDb; P49988; -.
DR PRIDE; P49988; -.
DR EnsemblBacteria; AAG07850; AAG07850; PA4462.
DR GeneID; 881022; -.
DR KEGG; pae:PA4462; -.
DR PATRIC; fig|208964.12.peg.4672; -.
DR PseudoCAP; PA4462; -.
DR HOGENOM; CLU_020569_0_1_6; -.
DR InParanoid; P49988; -.
DR OMA; VTTQKFM; -.
DR PhylomeDB; P49988; -.
DR BioCyc; PAER208964:G1FZ6-4551-MON; -.
DR PHI-base; PHI:7554; -.
DR Proteomes; UP000002438; Chromosome.
DR CollecTF; EXPREG_00000ad0; -.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0032993; C:protein-DNA complex; IMP:CollecTF.
DR GO; GO:0001216; F:DNA-binding transcription activator activity; IMP:CollecTF.
DR GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IMP:CollecTF.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR GO; GO:1900191; P:negative regulation of single-species biofilm formation; IMP:PseudoCAP.
DR Gene3D; 1.10.10.1330; -; 1.
DR InterPro; IPR000394; RNA_pol_sigma_54.
DR InterPro; IPR007046; RNA_pol_sigma_54_core-bd.
DR InterPro; IPR007634; RNA_pol_sigma_54_DNA-bd.
DR InterPro; IPR038709; RpoN_core-bd_sf.
DR PANTHER; PTHR32248; PTHR32248; 1.
DR Pfam; PF00309; Sigma54_AID; 1.
DR Pfam; PF04963; Sigma54_CBD; 1.
DR Pfam; PF04552; Sigma54_DBD; 1.
DR PIRSF; PIRSF000774; RpoN; 1.
DR PRINTS; PR00045; SIGMA54FCT.
DR TIGRFAMs; TIGR02395; rpoN_sigma; 1.
DR PROSITE; PS00717; SIGMA54_1; 1.
DR PROSITE; PS00718; SIGMA54_2; 1.
DR PROSITE; PS50044; SIGMA54_3; 1.
PE 1: Evidence at protein level;
KW DNA-binding; DNA-directed RNA polymerase; Nucleotidyltransferase;
KW Reference proteome; Sigma factor; Transcription; Transcription regulation;
KW Transferase.
FT CHAIN 1..497
FT /note="RNA polymerase sigma-54 factor"
FT /id="PRO_0000205534"
FT DNA_BIND 386..405
FT /note="H-T-H motif"
FT /evidence="ECO:0000255"
FT REGION 42..100
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 474..482
FT /note="RPON box"
FT COMPBIAS 72..89
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 352
FT /note="S -> T (in Ref. 1; AAA19793)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 497 AA; 56054 MW; CE1994F03FD58405 CRC64;
MKPSLVLKMG QQLTMTPQLQ QAIRLLQLST LDLQQEIQEA LESNPMLERQ EDGDDFDNSD
PLADGAEQAA SAPQESPLQE SATPSVESLD DDQWSERIPS ELPVDTAWED IYQTSASSLP
SNDDDEWDFT ARTSSGESLH SHLLWQVNLA PMSDTDRMIA VTIIDSINND GYLEESLEEI
LAAIDPELDV ELDEVEVVLR RIQQLEPAGI GARNLRECLL LQLRQLPSTT PWLNEALRLV
SDYLDLLGGR DYSQLMRRMK LKEDELRQVI ELIQCLHPRP GSQIESSEAE YIVPDVIVRK
DNERWLVELN QEAMPRLRVN ATYAGMVRRA DSSADNTFMR NQLQEARWFI KSLQSRNETL
MKVATQIVEH QRGFLDYGEE AMKPLVLHDI AEAVGMHEST ISRVTTQKYM HTPRGIFELK
YFFSSHVSTA EGGECSSTAI RAIIKKLVAA ENAKKPLSDS KIAGLLEAQG IQVARRTVAK
YRESLGIAPS SERKRLV
