RP54_PSEPK
ID RP54_PSEPK Reviewed; 497 AA.
AC P0A171; P15591;
DT 01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2005, sequence version 1.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=RNA polymerase sigma-54 factor;
GN Name=rpoN; Synonyms=ntrA; OrderedLocusNames=PP_0952;
OS Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950
OS / KT2440).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=160488;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2602128; DOI=10.1093/nar/17.23.10125;
RA Koehler T., Cayrol J.M., Ramos J.L., Harayama S.;
RT "Nucleotide and deduced amino acid sequence of the RpoN sigma-factor of
RT Pseudomonas putida.";
RL Nucleic Acids Res. 17:10125-10125(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440;
RX PubMed=12534463; DOI=10.1046/j.1462-2920.2002.00366.x;
RA Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H.,
RA Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M.,
RA Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F.,
RA Madupu R., Nelson W.C., White O., Peterson J.D., Khouri H.M., Hance I.,
RA Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A.,
RA Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H.,
RA Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C.,
RA Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M.;
RT "Complete genome sequence and comparative analysis of the metabolically
RT versatile Pseudomonas putida KT2440.";
RL Environ. Microbiol. 4:799-808(2002).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. This sigma factor is responsible for the expression of the
CC xylCAB operon and the xylS gene. The open complex (sigma-54 and core
CC RNA polymerase) serves as the receptor for receipt of the melting
CC signal from the remotely bound activator protein XylR for the
CC expression of the xylCAB operon and xylS.
CC -!- INTERACTION:
CC P0A171; P0A171: rpoN; NbExp=6; IntAct=EBI-8302876, EBI-8302876;
CC -!- SIMILARITY: Belongs to the sigma-54 factor family. {ECO:0000305}.
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DR EMBL; X16474; CAA34494.1; -; Genomic_DNA.
DR EMBL; AE015451; AAN66577.1; -; Genomic_DNA.
DR PIR; T01753; T01753.
DR RefSeq; NP_743113.1; NC_002947.4.
DR RefSeq; WP_003255133.1; NC_002947.4.
DR AlphaFoldDB; P0A171; -.
DR SMR; P0A171; -.
DR MINT; P0A171; -.
DR STRING; 160488.PP_0952; -.
DR PRIDE; P0A171; -.
DR EnsemblBacteria; AAN66577; AAN66577; PP_0952.
DR KEGG; ppu:PP_0952; -.
DR PATRIC; fig|160488.4.peg.1013; -.
DR eggNOG; COG1508; Bacteria.
DR HOGENOM; CLU_020569_0_1_6; -.
DR OMA; VTTQKFM; -.
DR PhylomeDB; P0A171; -.
DR BioCyc; PPUT160488:G1G01-1026-MON; -.
DR Proteomes; UP000000556; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0032993; C:protein-DNA complex; IPI:CollecTF.
DR GO; GO:0001216; F:DNA-binding transcription activator activity; IPI:CollecTF.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:CollecTF.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:CollecTF.
DR Gene3D; 1.10.10.1330; -; 1.
DR InterPro; IPR000394; RNA_pol_sigma_54.
DR InterPro; IPR007046; RNA_pol_sigma_54_core-bd.
DR InterPro; IPR007634; RNA_pol_sigma_54_DNA-bd.
DR InterPro; IPR038709; RpoN_core-bd_sf.
DR PANTHER; PTHR32248; PTHR32248; 1.
DR Pfam; PF00309; Sigma54_AID; 1.
DR Pfam; PF04963; Sigma54_CBD; 1.
DR Pfam; PF04552; Sigma54_DBD; 1.
DR PIRSF; PIRSF000774; RpoN; 1.
DR PRINTS; PR00045; SIGMA54FCT.
DR TIGRFAMs; TIGR02395; rpoN_sigma; 1.
DR PROSITE; PS00717; SIGMA54_1; 1.
DR PROSITE; PS00718; SIGMA54_2; 1.
DR PROSITE; PS50044; SIGMA54_3; 1.
PE 1: Evidence at protein level;
KW DNA-binding; DNA-directed RNA polymerase; Nucleotidyltransferase;
KW Reference proteome; Sigma factor; Transcription; Transcription regulation;
KW Transferase.
FT CHAIN 1..497
FT /note="RNA polymerase sigma-54 factor"
FT /id="PRO_0000205535"
FT DNA_BIND 386..405
FT /note="H-T-H motif"
FT /evidence="ECO:0000255"
FT REGION 41..101
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 474..482
FT /note="RPON box"
FT COMPBIAS 71..89
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 181
FT /note="C -> S (in Ref. 1; CAA34494)"
FT /evidence="ECO:0000305"
FT CONFLICT 366
FT /note="Q -> R (in Ref. 1; CAA34494)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 497 AA; 56215 MW; 921E788B48B5B2F8 CRC64;
MKPSLVLKMG QQLTMTPQLQ QAIRLLQLST LDLQQEIQEA LESNPMLERQ EDGEDFDNSD
PMADNAENKP AAEVQDNSFQ ESTVSADNLE DGEWSERIPN ELPVDTAWED IYQTSASSLP
SNDDDEWDFT TRTSAGESLQ SHLLWQLNLA PMSDTDRLIA VTLIDSINGQ GYLEDTLEEI
CAGFDPELDI ELDEVEAVLH RIQQFEPAGV GARNLGECLL LQLRQLPATT PWMTEAKRLV
TDFIDLLGSR DYSQLMRRMK IKEDELRQVI ELVQSLNPRP GSQIESSEPE YVVPDVIVRK
DSDRWLVELN QEAIPRLRVN PQYAGFVRRA DTSADNTFMR NQLQEARWFI KSLQSRNETL
MKVATQIVEH QRGFLDHGDE AMKPLVLHDI AEAVGMHEST ISRVTTQKYM HTPRGIYELK
YFFSSHVSTS EGGECSSTAI RAIIKKLVAA ENQKKPLSDS KIAGLLEAQG IQVARRTVAK
YRESLGIAPS SERKRLM
