ID   RP54_PSEPU              Reviewed;         497 AA.
AC   P0A172; P15591;
DT   01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 57.
DE   RecName: Full=RNA polymerase sigma-54 factor;
GN   Name=rpoN; Synonyms=ntrA;
OS   Pseudomonas putida (Arthrobacter siderocapsulatus).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=303;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=TN2100;
RX   PubMed=2695395; DOI=10.1016/0378-1119(89)90474-5;
RA   Inouye S., Yamada M., Nakazawa A., Nakazawa T.;
RT   "Cloning and sequence analysis of the ntrA (rpoN) gene of Pseudomonas
RT   putida.";
RL   Gene 85:145-152(1989).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. This sigma factor is responsible for the expression of the
CC       xylCAB operon and the xylS gene. The open complex (sigma-54 and core
CC       RNA polymerase) serves as the receptor for receipt of the melting
CC       signal from the remotely bound activator protein XylR for the
CC       expression of the xylCAB operon and xylS.
CC   -!- SIMILARITY: Belongs to the sigma-54 factor family. {ECO:0000305}.
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DR   EMBL; M24916; AAA88444.1; -; Genomic_DNA.
DR   PIR; JQ0338; JQ0338.
DR   PIR; S15918; RNPSSN.
DR   RefSeq; WP_003255133.1; NZ_SCFX01000028.1.
DR   AlphaFoldDB; P0A172; -.
DR   SMR; P0A172; -.
DR   STRING; 1240350.AMZE01000051_gene865; -.
DR   eggNOG; COG1508; Bacteria.
DR   OMA; VTTQKFM; -.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0001216; F:DNA-binding transcription activator activity; IEA:InterPro.
DR   GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR   Gene3D; 1.10.10.1330; -; 1.
DR   InterPro; IPR000394; RNA_pol_sigma_54.
DR   InterPro; IPR007046; RNA_pol_sigma_54_core-bd.
DR   InterPro; IPR007634; RNA_pol_sigma_54_DNA-bd.
DR   InterPro; IPR038709; RpoN_core-bd_sf.
DR   PANTHER; PTHR32248; PTHR32248; 1.
DR   Pfam; PF00309; Sigma54_AID; 1.
DR   Pfam; PF04963; Sigma54_CBD; 1.
DR   Pfam; PF04552; Sigma54_DBD; 1.
DR   PIRSF; PIRSF000774; RpoN; 1.
DR   PRINTS; PR00045; SIGMA54FCT.
DR   TIGRFAMs; TIGR02395; rpoN_sigma; 1.
DR   PROSITE; PS00717; SIGMA54_1; 1.
DR   PROSITE; PS00718; SIGMA54_2; 1.
DR   PROSITE; PS50044; SIGMA54_3; 1.
PE   3: Inferred from homology;
KW   DNA-binding; DNA-directed RNA polymerase; Nucleotidyltransferase;
KW   Sigma factor; Transcription; Transcription regulation; Transferase.
FT   CHAIN           1..497
FT                   /note="RNA polymerase sigma-54 factor"
FT                   /id="PRO_0000205536"
FT   DNA_BIND        386..405
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255"
FT   REGION          41..101
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           474..482
FT                   /note="RPON box"
FT   COMPBIAS        71..89
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   497 AA;  56215 MW;  921E788B48B5B2F8 CRC64;
     MKPSLVLKMG QQLTMTPQLQ QAIRLLQLST LDLQQEIQEA LESNPMLERQ EDGEDFDNSD
     PMADNAENKP AAEVQDNSFQ ESTVSADNLE DGEWSERIPN ELPVDTAWED IYQTSASSLP
     SNDDDEWDFT TRTSAGESLQ SHLLWQLNLA PMSDTDRLIA VTLIDSINGQ GYLEDTLEEI
     CAGFDPELDI ELDEVEAVLH RIQQFEPAGV GARNLGECLL LQLRQLPATT PWMTEAKRLV
     TDFIDLLGSR DYSQLMRRMK IKEDELRQVI ELVQSLNPRP GSQIESSEPE YVVPDVIVRK
     DSDRWLVELN QEAIPRLRVN PQYAGFVRRA DTSADNTFMR NQLQEARWFI KSLQSRNETL
     MKVATQIVEH QRGFLDHGDE AMKPLVLHDI AEAVGMHEST ISRVTTQKYM HTPRGIYELK
     YFFSSHVSTS EGGECSSTAI RAIIKKLVAA ENQKKPLSDS KIAGLLEAQG IQVARRTVAK
     YRESLGIAPS SERKRLM