ID   RP54_RHIET              Reviewed;         520 AA.
AC   P49989;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=RNA polymerase sigma-54 factor;
GN   Name=rpoN; Synonyms=ntrA;
OS   Rhizobium etli.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium.
OX   NCBI_TaxID=29449;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=CNPAF512;
RX   PubMed=9537369; DOI=10.1128/jb.180.7.1729-1740.1998;
RA   de Wilde P., Vanderleyden J.;
RT   "The Rhizobium etli rpoN locus: DNA sequence analysis and phenotypical
RT   characterization of rpoN, ptsN, and ptsA mutants.";
RL   J. Bacteriol. 180:1729-1740(1998).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. This sigma factor is responsible for the expression of the
CC       nitrogen fixation genes (nif operon), GlnA and DctA for dicarboxylate
CC       transport. The open complex (sigma-54 and core RNA polymerase) serves
CC       as the receptor for receipt of the melting signal from the remotely
CC       bound activator proteins NifA, NtrC, or DctD for the expression of the
CC       regulated proteins (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the sigma-54 factor family. {ECO:0000305}.
CC   -!- CAUTION: Strain CNPAF512 was originally thought to originate from
CC       R.leguminosarum bv phaseoli. {ECO:0000305}.
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DR   EMBL; U23471; AAA80300.1; -; Genomic_DNA.
DR   AlphaFoldDB; P49989; -.
DR   SMR; P49989; -.
DR   STRING; 491916.RHECIAT_CH0000446; -.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0001216; F:DNA-binding transcription activator activity; IEA:InterPro.
DR   GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR   GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.1330; -; 1.
DR   InterPro; IPR000394; RNA_pol_sigma_54.
DR   InterPro; IPR007046; RNA_pol_sigma_54_core-bd.
DR   InterPro; IPR007634; RNA_pol_sigma_54_DNA-bd.
DR   InterPro; IPR038709; RpoN_core-bd_sf.
DR   PANTHER; PTHR32248; PTHR32248; 1.
DR   Pfam; PF00309; Sigma54_AID; 1.
DR   Pfam; PF04963; Sigma54_CBD; 1.
DR   Pfam; PF04552; Sigma54_DBD; 1.
DR   PIRSF; PIRSF000774; RpoN; 1.
DR   PRINTS; PR00045; SIGMA54FCT.
DR   TIGRFAMs; TIGR02395; rpoN_sigma; 1.
DR   PROSITE; PS00717; SIGMA54_1; 1.
DR   PROSITE; PS00718; SIGMA54_2; 1.
DR   PROSITE; PS50044; SIGMA54_3; 1.
PE   3: Inferred from homology;
KW   DNA-binding; DNA-directed RNA polymerase; Nitrogen fixation;
KW   Nucleotidyltransferase; Sigma factor; Transcription;
KW   Transcription regulation; Transferase.
FT   CHAIN           1..520
FT                   /note="RNA polymerase sigma-54 factor"
FT                   /id="PRO_0000205537"
FT   DNA_BIND        398..417
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255"
FT   REGION          49..107
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           488..496
FT                   /note="RPON box"
FT   COMPBIAS        55..69
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   520 AA;  57478 MW;  3CB8A6358120A93D CRC64;
     MALSANLFLR QSQSLVMTPQ LMQSIQLLQM THFELNQFIA QEVEKNPLLE FPSNDGEAGD
     ERGAGEDEPF GQPSEDAGSD DGADSRAEAL SSDWYDNGGS ESTGRLNDEL DANYTNVFPD
     DGGPQRLDAP ELVGQWKSMP GSAEGADYDL DDFVAGQLSL RDHLAQQIPF VLPDMTDRLI
     AQNFVDQLDD AGYLQTDLVE TGERLGTSLE QVEHVLAALQ TLDPPGVFAR SLAECLAIQL
     RQKDRYDPAM QALVANLELL ARRDFATLKR LCGVDEEDLL DMLGEIRQLN PKPGGGFEAG
     VSEAIMPDVV VRPSADGGWL VELNPDTLPR VLVNQSYFSR VTRNGEDHAF LSECLQSANW
     LTRTLDQRAK TIMKVASEIV RQQDAFLLNG VDHLRPLNLK TVAEAIKMHE STVSRVTSNK
     YMLTPRGLFE LKYFFTVSIS AVEGGEQHSA EAVRATRFRA LIMQESPDAV LSDDDIVDML
     KKGGIDLARR TVAKYREAMN IASSVQRRRE KRALAKVAGF