RP54_RHIME
ID RP54_RHIME Reviewed; 514 AA.
AC P17263; Q59751;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2000, sequence version 2.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=RNA polymerase sigma-54 factor;
GN Name=rpoN; Synonyms=ntrA; OrderedLocusNames=R00381; ORFNames=SMc01139;
OS Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS meliloti).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX NCBI_TaxID=266834;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3034856; DOI=10.1128/jb.169.6.2424-2431.1987;
RA Ronson C.W., Nixon B.T., Albright L.M., Ausubel F.M.;
RT "Rhizobium meliloti ntrA (rpoN) gene is required for diverse metabolic
RT functions.";
RL J. Bacteriol. 169:2424-2431(1987).
RN [2]
RP SEQUENCE REVISION TO C-TERMINUS.
RX PubMed=2703463; DOI=10.1128/jb.171.4.1932-1941.1989;
RA Albright L.M., Ronson C.W., Nixon B.T., Ausubel F.M.;
RT "Identification of a gene linked to Rhizobium meliloti ntrA whose product
RT is homologous to a family to ATP-binding proteins.";
RL J. Bacteriol. 171:1932-1941(1989).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11481430; DOI=10.1073/pnas.161294398;
RA Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J.,
RA Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S.,
RA Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D., Pohl T.,
RA Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C.,
RA Thebault P., Vandenbol M., Weidner S., Galibert F.;
RT "Analysis of the chromosome sequence of the legume symbiont Sinorhizobium
RT meliloti strain 1021.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11474104; DOI=10.1126/science.1060966;
RA Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S.,
RA Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I.,
RA Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S.,
RA Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C.,
RA Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R.,
RA Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H.,
RA Wong K., Yeh K.-C., Batut J.;
RT "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL Science 293:668-672(2001).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. This sigma factor is responsible for the expression of the
CC nitrogen fixation genes (nif operon), glnA and dctA for dicarboxylate
CC transport. The open complex (sigma-54 and core RNA polymerase) serves
CC as the receptor for receipt of the melting signal from the remotely
CC bound activator proteins NifA, NtrC, or DctD for the expression of the
CC regulated proteins.
CC -!- SIMILARITY: Belongs to the sigma-54 factor family. {ECO:0000305}.
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DR EMBL; M16513; AAD15117.1; -; Genomic_DNA.
DR EMBL; M24926; AAA26349.1; -; Genomic_DNA.
DR EMBL; AL591688; CAC41818.1; -; Genomic_DNA.
DR RefSeq; NP_384487.1; NC_003047.1.
DR RefSeq; WP_010968540.1; NC_003047.1.
DR AlphaFoldDB; P17263; -.
DR SMR; P17263; -.
DR STRING; 266834.SMc01139; -.
DR EnsemblBacteria; CAC41818; CAC41818; SMc01139.
DR GeneID; 61601860; -.
DR KEGG; sme:SMc01139; -.
DR PATRIC; fig|266834.11.peg.1753; -.
DR eggNOG; COG1508; Bacteria.
DR HOGENOM; CLU_020569_0_0_5; -.
DR OMA; VTTQKFM; -.
DR Proteomes; UP000001976; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0001216; F:DNA-binding transcription activator activity; IEA:InterPro.
DR GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR Gene3D; 1.10.10.1330; -; 1.
DR InterPro; IPR000394; RNA_pol_sigma_54.
DR InterPro; IPR007046; RNA_pol_sigma_54_core-bd.
DR InterPro; IPR007634; RNA_pol_sigma_54_DNA-bd.
DR InterPro; IPR038709; RpoN_core-bd_sf.
DR PANTHER; PTHR32248; PTHR32248; 1.
DR Pfam; PF00309; Sigma54_AID; 1.
DR Pfam; PF04963; Sigma54_CBD; 1.
DR Pfam; PF04552; Sigma54_DBD; 1.
DR PIRSF; PIRSF000774; RpoN; 1.
DR PRINTS; PR00045; SIGMA54FCT.
DR TIGRFAMs; TIGR02395; rpoN_sigma; 1.
DR PROSITE; PS00717; SIGMA54_1; 1.
DR PROSITE; PS00718; SIGMA54_2; 1.
DR PROSITE; PS50044; SIGMA54_3; 1.
PE 3: Inferred from homology;
KW DNA-binding; DNA-directed RNA polymerase; Nitrogen fixation;
KW Nucleotidyltransferase; Reference proteome; Sigma factor; Transcription;
KW Transcription regulation; Transferase.
FT CHAIN 1..514
FT /note="RNA polymerase sigma-54 factor"
FT /id="PRO_0000205538"
FT DNA_BIND 393..412
FT /note="H-T-H motif"
FT /evidence="ECO:0000255"
FT REGION 57..83
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 482..490
FT /note="RPON box"
FT COMPBIAS 57..71
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 509..514
FT /note="AKAAGF -> PRPRDSERCRQAASA (in Ref. 1)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 514 AA; 56679 MW; C291DAE1D2DD034C CRC64;
MALSASLHLR QSQSLVMTPQ LMQSIQLLQM NHLELSHFIA QEVEKNPLLE VQPADEPTIS
DREDAGPHPA ETGGETDEAA GQSDLYDSAM SRSGERLSEG LDADFANVFP DDTAPQRADA
PELLGQWKSM PGAGDAEGYD LDDFVGGRKT LRETLAEQLP FALSAVSDRL IARYFIDQLD
DAGYLHADLA ETAETLGAAG EDVARVLHVL QQFDPPGVFA RTLGECLAIQ LRARNRLDPA
MEALVANLEL LARRDFASLK KICGVDEEDL IDMLAEIRKL DPKPGTSFET GVFEAIIPDV
VVRAAPDGGW LVELNPDALP RVLVNHDYFT EISRSSRKNS GEQAFLNECL QNANWLTRSL
DQRARTIMKV ASEIVRQQDA FLIHGVGHLR PLNLRIVADA IKMHESTVSR VTSNKYMLTP
RGLFELKYFF TVSIGSAENG DAHSAESVRH RIRTMINQES ADAVLSDDDI VDVLKQAGVD
IARRTVAKYR EAMSIPSSVQ RRREKRALAK AAGF
