RP54_RHIML
ID RP54_RHIML Reviewed; 513 AA.
AC P33985;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 1.
DT 03-AUG-2022, entry version 87.
DE RecName: Full=RNA polymerase sigma-54 factor;
GN Name=rpoN; Synonyms=ntrA;
OS Rhizobium meliloti (Ensifer meliloti) (Sinorhizobium meliloti).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX NCBI_TaxID=382;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=104A14;
RX PubMed=2570059; DOI=10.1128/jb.171.9.5087-5094.1989;
RA Shatters R.G., Somerville J.E., Kahn M.L.;
RT "Regulation of glutamine synthetase II activity in Rhizobium meliloti
RT 104A14.";
RL J. Bacteriol. 171:5087-5094(1989).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. This sigma factor is responsible for the expression of the
CC nitrogen fixation genes (nif operon), glnA and dctA for dicarboxylate
CC transport. The open complex (sigma-54 and core RNA polymerase) serves
CC as the receptor for receipt of the melting signal from the remotely
CC bound activator proteins NifA, NtrC, or DctD for the expression of the
CC regulated proteins.
CC -!- SIMILARITY: Belongs to the sigma-54 factor family. {ECO:0000305}.
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DR EMBL; M28846; AAA26244.1; -; Genomic_DNA.
DR PIR; A33966; A33966.
DR AlphaFoldDB; P33985; -.
DR SMR; P33985; -.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0001216; F:DNA-binding transcription activator activity; IEA:InterPro.
DR GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR Gene3D; 1.10.10.1330; -; 1.
DR InterPro; IPR000394; RNA_pol_sigma_54.
DR InterPro; IPR007046; RNA_pol_sigma_54_core-bd.
DR InterPro; IPR007634; RNA_pol_sigma_54_DNA-bd.
DR InterPro; IPR038709; RpoN_core-bd_sf.
DR PANTHER; PTHR32248; PTHR32248; 1.
DR Pfam; PF00309; Sigma54_AID; 1.
DR Pfam; PF04963; Sigma54_CBD; 1.
DR Pfam; PF04552; Sigma54_DBD; 1.
DR PIRSF; PIRSF000774; RpoN; 1.
DR PRINTS; PR00045; SIGMA54FCT.
DR TIGRFAMs; TIGR02395; rpoN_sigma; 1.
DR PROSITE; PS00717; SIGMA54_1; 1.
DR PROSITE; PS00718; SIGMA54_2; 1.
DR PROSITE; PS50044; SIGMA54_3; 1.
PE 3: Inferred from homology;
KW DNA-binding; DNA-directed RNA polymerase; Nitrogen fixation;
KW Nucleotidyltransferase; Sigma factor; Transcription;
KW Transcription regulation; Transferase.
FT CHAIN 1..513
FT /note="RNA polymerase sigma-54 factor"
FT /id="PRO_0000205539"
FT DNA_BIND 394..413
FT /note="H-T-H motif"
FT /evidence="ECO:0000255"
FT REGION 52..137
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 483..491
FT /note="RPON box"
SQ SEQUENCE 513 AA; 56822 MW; EA806901BC8EE5BB CRC64;
MALSASLHLR QSQSLVMTPQ LMQSIQLLQM NHLELTQFIA QEIEKNPLLE VQSPSDEAST
AERGDSGPQP EEAGSEIDEG AGEGDVYDSA TSRSGERLSD GLDSDFANVF PDDTTPQRAD
APELLGQWKS MPGASDGESY DLDDFVASRK TLREALIEQL PFALESGSHR LIAQYLIDQL
DDAGYLHADL AETAQRLASA SEDVTRVLDV LQQFDPPGVF ARTLGECLGL QLRARNRLDP
AMEALVGNLD LLARRDFASL KKICGVDEED LIDMFAEIRK LDPKPGTSFE TGSFETIIPD
AVVRTAPDGG WLVELNPDAL PRVLVNHEYF AEISRSCRKS SGEQIFLNEC LQNANWLTRS
LDQRARTIMK VASEIVRQQD AFLMHGVGHL RPLNLRTVAD AIKMHESTVS RVTSNKYMLT
PRGLYELKYF FTASIGSAEN GDAHSAESVR HRIRTMVNQE SADAVLSDDD IVDILKKAGV
DIARRTVAKY REAMHIPSCV QRRREKRALA RVG
