ID   RP54_RHOCA              Reviewed;         426 AA.
AC   P0CY59; P09433;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   03-AUG-2022, entry version 31.
DE   RecName: Full=RNA polymerase sigma-54 factor;
GN   Name=rpoN; Synonyms=nifR4, ntrA;
OS   Rhodobacter capsulatus (Rhodopseudomonas capsulata).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC   Rhodobacteraceae; Rhodobacter.
OX   NCBI_TaxID=1061;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 33303 / B10;
RX   PubMed=2762129; DOI=10.1093/nar/17.13.5377;
RA   Alais A., Cejudo F.J., Chabert J., Willison J.C., Vignais P.M.;
RT   "Nucleotide sequence of wild-type and mutant nifR4 (ntrA) genes of
RT   Rhodobacter capsulatus: identification of an essential glycine residue.";
RL   Nucleic Acids Res. 17:5377-5377(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-10.
RC   STRAIN=B10S;
RX   PubMed=1374827; DOI=10.1111/j.1365-2958.1992.tb02169.x;
RA   Preker P., Huebner P., Schmehl M., Klipp W., Bickle T.A.;
RT   "Mapping and characterization of the promoter elements of the regulatory
RT   nif genes rpoN, nifA1 and nifA2 in Rhodobacter capsulatus.";
RL   Mol. Microbiol. 6:1035-1047(1992).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. This sigma factor is responsible for the expression of the
CC       nitrogen fixation genes. The open complex (sigma-54 and core RNA
CC       polymerase) serves as the receptor for receipt of the melting signal
CC       from the remotely bound activator protein nifA for the expression of
CC       the nitrogen fixation proteins.
CC   -!- SIMILARITY: Belongs to the sigma-54 factor family. {ECO:0000305}.
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DR   EMBL; X15437; CAA33477.1; -; Genomic_DNA.
DR   EMBL; X63196; CAA44880.1; -; Genomic_DNA.
DR   PIR; S04848; S04848.
DR   AlphaFoldDB; P0CY59; -.
DR   SMR; P0CY59; -.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0001216; F:DNA-binding transcription activator activity; IEA:InterPro.
DR   GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR   GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.1330; -; 1.
DR   InterPro; IPR000394; RNA_pol_sigma_54.
DR   InterPro; IPR007046; RNA_pol_sigma_54_core-bd.
DR   InterPro; IPR007634; RNA_pol_sigma_54_DNA-bd.
DR   InterPro; IPR038709; RpoN_core-bd_sf.
DR   PANTHER; PTHR32248; PTHR32248; 1.
DR   Pfam; PF00309; Sigma54_AID; 1.
DR   Pfam; PF04963; Sigma54_CBD; 1.
DR   Pfam; PF04552; Sigma54_DBD; 1.
DR   PIRSF; PIRSF000774; RpoN; 1.
DR   PRINTS; PR00045; SIGMA54FCT.
DR   TIGRFAMs; TIGR02395; rpoN_sigma; 1.
DR   PROSITE; PS00717; SIGMA54_1; 1.
DR   PROSITE; PS00718; SIGMA54_2; 1.
DR   PROSITE; PS50044; SIGMA54_3; 1.
PE   3: Inferred from homology;
KW   DNA-binding; DNA-directed RNA polymerase; Nitrogen fixation;
KW   Nucleotidyltransferase; Sigma factor; Transcription;
KW   Transcription regulation; Transferase.
FT   CHAIN           1..426
FT                   /note="RNA polymerase sigma-54 factor"
FT                   /id="PRO_0000205541"
FT   DNA_BIND        312..331
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255"
FT   REGION          257..278
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           400..408
FT                   /note="RPON box"
SQ   SEQUENCE   426 AA;  46230 MW;  2A9852B691ED456D CRC64;
     MELAQTLSQR QTMQMAGQML HSLAILGMSS QDLSEHLTEQ ATSNPFLTYR APPAFIARGG
     EDFDAVGAVA AHKPSLMAHV VDQIEMAFTE TPDRLLALRF AEALEPSGWL GQSLDSIALA
     AGVSLSRAES MLAVLQGFEP TGLFARDLSD CLILQAREAD ILTWEVETLI RNIRLIAENR
     LSDLADLCDC DIGDIPEIIK QIRHLNPKPG LAFDHQPTPV FPPDLIAVRG AEGWTVELNR
     ATSPTITVRE DRFADGTADA KARAERRRRG RGPGAGEALE RRGDTLLRTA AVLVARQSAF
     LDKGPAHLVP LTLEDVASEL GLHASTISRA VSGRMIQTQT RALPLRAFFS RAVSTQGGGE
     AVSRDSLDFV QRTVGGEDRQ NPLSDDAIVT LAERAGLRIA RRTVAKYRST LGLASSYERR
     RAAAAR