ID   RP54_SALTY              Reviewed;         477 AA.
AC   P26979;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   03-AUG-2022, entry version 133.
DE   RecName: Full=RNA polymerase sigma-54 factor;
GN   Name=rpoN; Synonyms=glnF, ntrA; OrderedLocusNames=STM3320;
OS   Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=99287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1918063; DOI=10.1016/s0021-9258(18)55025-0;
RA   Popham D.L., Keener J., Kustu S.;
RT   "Purification of the alternative sigma factor, sigma 54, from Salmonella
RT   typhimurium and characterization of sigma 54-holoenzyme.";
RL   J. Biol. Chem. 266:19510-19518(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX   PubMed=11677609; DOI=10.1038/35101614;
RA   McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA   Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA   Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA   Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA   Wilson R.K.;
RT   "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL   Nature 413:852-856(2001).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. This sigma factor is responsible for the expression of
CC       enzymes involved in arginine catabolism. The open complex (sigma-54 and
CC       core RNA polymerase) serves as the receptor for the receipt of the
CC       melting signal from the remotely bound activator protein GlnG(NtrC).
CC   -!- SIMILARITY: Belongs to the sigma-54 factor family. {ECO:0000305}.
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DR   EMBL; M68571; AAA27224.1; -; Genomic_DNA.
DR   EMBL; AE006468; AAL22189.1; -; Genomic_DNA.
DR   PIR; A41026; A41026.
DR   RefSeq; NP_462230.1; NC_003197.2.
DR   RefSeq; WP_000809020.1; NC_003197.2.
DR   AlphaFoldDB; P26979; -.
DR   BMRB; P26979; -.
DR   SMR; P26979; -.
DR   MINT; P26979; -.
DR   STRING; 99287.STM3320; -.
DR   PaxDb; P26979; -.
DR   EnsemblBacteria; AAL22189; AAL22189; STM3320.
DR   GeneID; 1254843; -.
DR   KEGG; stm:STM3320; -.
DR   PATRIC; fig|99287.12.peg.3521; -.
DR   HOGENOM; CLU_020569_0_1_6; -.
DR   OMA; VTTQKFM; -.
DR   PhylomeDB; P26979; -.
DR   BioCyc; SENT99287:STM3320-MON; -.
DR   Proteomes; UP000001014; Chromosome.
DR   CollecTF; EXPREG_000009e0; -.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0001216; F:DNA-binding transcription activator activity; IEA:InterPro.
DR   GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0006525; P:arginine metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR   Gene3D; 1.10.10.1330; -; 1.
DR   InterPro; IPR000394; RNA_pol_sigma_54.
DR   InterPro; IPR007046; RNA_pol_sigma_54_core-bd.
DR   InterPro; IPR007634; RNA_pol_sigma_54_DNA-bd.
DR   InterPro; IPR038709; RpoN_core-bd_sf.
DR   PANTHER; PTHR32248; PTHR32248; 1.
DR   Pfam; PF00309; Sigma54_AID; 1.
DR   Pfam; PF04963; Sigma54_CBD; 1.
DR   Pfam; PF04552; Sigma54_DBD; 1.
DR   PIRSF; PIRSF000774; RpoN; 1.
DR   PRINTS; PR00045; SIGMA54FCT.
DR   TIGRFAMs; TIGR02395; rpoN_sigma; 1.
DR   PROSITE; PS00717; SIGMA54_1; 1.
DR   PROSITE; PS00718; SIGMA54_2; 1.
DR   PROSITE; PS50044; SIGMA54_3; 1.
PE   3: Inferred from homology;
KW   Arginine metabolism; DNA-binding; DNA-directed RNA polymerase;
KW   Nucleotidyltransferase; Reference proteome; Sigma factor; Transcription;
KW   Transcription regulation; Transferase.
FT   CHAIN           1..477
FT                   /note="RNA polymerase sigma-54 factor"
FT                   /id="PRO_0000205530"
FT   DNA_BIND        366..385
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   MOTIF           454..462
FT                   /note="RPON box"
SQ   SEQUENCE   477 AA;  53988 MW;  5077B24BA8BAC8FF CRC64;
     MKQGLQLRLS QQLAMTPQLQ QAIRLLQLST LELQQELQQA LENNPLLEQT DLHDEIDTQQ
     PQDNDPLDTA DALEQKEMPE ELPLDASWDE IYTAGTPSGP SGDYIDDELP VYQGETTQSL
     QDYLMWQVEL TPFSDTDRAI ATSIVDAVDD TGYLTVSLDE IRESMGDVEV DLDEVEAVLK
     RIQRFDPVGV AAKDLRDCLL IQLSQFDKST PWLEEARLII CDHLDLLANH DFRTLMRVTR
     LKEEVLKEAV NLIQSLDPRP GQSIQTGEPE YVIPDVLVRK HNGRWTVELN SDSIPRLQIN
     QHYAAMCNSA RNDADSQFIR SNLQDAKWLI KSLESRNDTL LRVSRCIVEQ QQAFFEQGEE
     YMKPMVLADI AQAVEMHEST ISRVTTQKYL HSPRGIFELK YFFSSHVNTE GGGEASSTAI
     RALVKKLIAA ENPAKPLSDS KLTSLLSEQG IMVARRTVAK YRESLSIPPS NQRKQLV