ID   RP54_SHEVD              Reviewed;         492 AA.
AC   Q9S0L2; D4ZF05;
DT   13-AUG-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=RNA polymerase sigma-54 factor;
GN   Name=rpoN; OrderedLocusNames=SVI_0414;
OS   Shewanella violacea (strain JCM 10179 / CIP 106290 / LMG 19151 / DSS12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC   Shewanellaceae; Shewanella.
OX   NCBI_TaxID=637905;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=10760597; DOI=10.1016/s0167-4781(00)00035-x;
RA   Ikegami A., Nakasone K., Fujita M., Fujii S., Kato C., Usami R.,
RA   Horikoshi K.;
RT   "Cloning and characterization of the gene encoding RNA polymerase sigma
RT   factor 54 of deep-sea piezophilic Shewanella violacea.";
RL   Biochim. Biophys. Acta 1491:315-320(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JCM 10179 / CIP 106290 / LMG 19151 / DSS12;
RX   PubMed=20458400; DOI=10.1039/c000396d;
RA   Aono E., Baba T., Ara T., Nishi T., Nakamichi T., Inamoto E., Toyonaga H.,
RA   Hasegawa M., Takai Y., Okumura Y., Baba M., Tomita M., Kato C., Oshima T.,
RA   Nakasone K., Mori H.;
RT   "Complete genome sequence and comparative analysis of Shewanella violacea,
RT   a psychrophilic and piezophilic bacterium from deep sea floor sediments.";
RL   Mol. Biosyst. 6:1216-1226(2010).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. This sigma factor recognizes a DNA element, designated as
CC       region A upstream of the pressure-regulated operon.
CC   -!- SIMILARITY: Belongs to the sigma-54 factor family. {ECO:0000305}.
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DR   EMBL; AB033988; BAA85885.1; -; Genomic_DNA.
DR   EMBL; AP011177; BAJ00385.1; -; Genomic_DNA.
DR   RefSeq; WP_013049699.1; NC_014012.1.
DR   AlphaFoldDB; Q9S0L2; -.
DR   SMR; Q9S0L2; -.
DR   STRING; 637905.SVI_0414; -.
DR   EnsemblBacteria; BAJ00385; BAJ00385; SVI_0414.
DR   KEGG; svo:SVI_0414; -.
DR   eggNOG; COG1508; Bacteria.
DR   HOGENOM; CLU_020569_0_1_6; -.
DR   OMA; VTTQKFM; -.
DR   OrthoDB; 1906842at2; -.
DR   Proteomes; UP000002350; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0001216; F:DNA-binding transcription activator activity; IEA:InterPro.
DR   GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR   Gene3D; 1.10.10.1330; -; 1.
DR   InterPro; IPR000394; RNA_pol_sigma_54.
DR   InterPro; IPR007046; RNA_pol_sigma_54_core-bd.
DR   InterPro; IPR007634; RNA_pol_sigma_54_DNA-bd.
DR   InterPro; IPR038709; RpoN_core-bd_sf.
DR   PANTHER; PTHR32248; PTHR32248; 1.
DR   Pfam; PF00309; Sigma54_AID; 1.
DR   Pfam; PF04963; Sigma54_CBD; 1.
DR   Pfam; PF04552; Sigma54_DBD; 1.
DR   PIRSF; PIRSF000774; RpoN; 1.
DR   PRINTS; PR00045; SIGMA54FCT.
DR   TIGRFAMs; TIGR02395; rpoN_sigma; 1.
DR   PROSITE; PS00717; SIGMA54_1; 1.
DR   PROSITE; PS00718; SIGMA54_2; 1.
DR   PROSITE; PS50044; SIGMA54_3; 1.
PE   3: Inferred from homology;
KW   DNA-binding; DNA-directed RNA polymerase; Nucleotidyltransferase;
KW   Reference proteome; Sigma factor; Transcription; Transcription regulation;
KW   Transferase.
FT   CHAIN           1..492
FT                   /note="RNA polymerase sigma-54 factor"
FT                   /id="PRO_0000205543"
FT   DNA_BIND        382..401
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   REGION          54..92
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           470..478
FT                   /note="RPON box"
FT   COMPBIAS        56..70
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        76..90
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   492 AA;  55360 MW;  8B95B22C706DF7F7 CRC64;
     MKASLQLKMG QQLTMTPQLQ QAIRLLQLSS LELQQEIQQA LDSNPLLELD EEQVDPPVNG
     EDKTVDTEDF SATAESDGPI DNSAVETSEA ITRDSMPEEL PMDTTWDEVY TASPNSTSGA
     MRDDDMPFQG ETSEGLYEHL EWQKNLTPFS DNDLAIATAI IEAIDERGYL TQSIEDILEA
     MGDPEIEQDE IEAVLKRIQH FDPIGIAARD LSECLLIQLA QYADTTPHID NARILIRDHL
     DLIAGRDFRL LMRKTKLKED ALRDAIELIQ TLNPRPGLAV TPGKDEYVIP DVTVTKKKGR
     WMVELNPDNM PKISVNQHYA SMAKSTKSQA DSQFIRGHLQ EAKWFIKSLE SRNETLLKVS
     KCIVKFQQGF FEFGEEAMKP MVLNDIAEAV EMHESTISRV TTQKYMHTPR GIFELKYFFS
     SHVATDDGGE CSSTAIRAFI KKLVAAENQQ KPLSDSKMAL LLADQGIKVA RRTIAKYREA
     MLIPPSNQRK SL