RP54_SINFN
ID RP54_SINFN Reviewed; 519 AA.
AC P22881; C3MEJ8;
DT 01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT 07-JUL-2009, sequence version 2.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=RNA polymerase sigma-54 factor;
GN Name=rpoN; Synonyms=ntrA; OrderedLocusNames=NGR_c00170;
OS Sinorhizobium fredii (strain NBRC 101917 / NGR234).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX NCBI_TaxID=394;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2211497; DOI=10.1128/jb.172.10.5563-5574.1990;
RA van Slooten J.C., Cervantes E., Broughton W.J., Wong C.H., Stanley J.;
RT "Sequence and analysis of the rpoN sigma factor gene of rhizobium sp.
RT strain NGR234, a primary coregulator of symbiosis.";
RL J. Bacteriol. 172:5563-5574(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NBRC 101917 / NGR234;
RX PubMed=19376903; DOI=10.1128/aem.00515-09;
RA Schmeisser C., Liesegang H., Krysciak D., Bakkou N., Le Quere A.,
RA Wollherr A., Heinemeyer I., Morgenstern B., Pommerening-Roeser A.,
RA Flores M., Palacios R., Brenner S., Gottschalk G., Schmitz R.A.,
RA Broughton W.J., Perret X., Strittmatter A.W., Streit W.R.;
RT "Rhizobium sp. strain NGR234 possesses a remarkable number of secretion
RT systems.";
RL Appl. Environ. Microbiol. 75:4035-4045(2009).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. This sigma factor is responsible for the expression of the
CC nitrogen fixation genes (nif operon), glnA and dctA for dicarboxylate
CC transport. The open complex (sigma-54 and core RNA polymerase) serves
CC as the receptor for receipt of the melting signal from the remotely
CC bound activator proteins NifA, NtrC, or DctD for the expression of the
CC regulated proteins.
CC -!- SIMILARITY: Belongs to the sigma-54 factor family. {ECO:0000305}.
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DR EMBL; M58481; AAA26361.1; -; Genomic_DNA.
DR EMBL; CP001389; ACP23821.1; -; Genomic_DNA.
DR PIR; A36130; A36130.
DR RefSeq; WP_012706606.1; NC_012587.1.
DR RefSeq; YP_002824574.1; NC_012587.1.
DR AlphaFoldDB; P22881; -.
DR SMR; P22881; -.
DR STRING; 394.NGR_c00170; -.
DR EnsemblBacteria; ACP23821; ACP23821; NGR_c00170.
DR KEGG; rhi:NGR_c00170; -.
DR PATRIC; fig|394.7.peg.2807; -.
DR eggNOG; COG1508; Bacteria.
DR HOGENOM; CLU_020569_0_0_5; -.
DR OMA; VTTQKFM; -.
DR OrthoDB; 1906842at2; -.
DR Proteomes; UP000001054; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0001216; F:DNA-binding transcription activator activity; IEA:InterPro.
DR GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR Gene3D; 1.10.10.1330; -; 1.
DR InterPro; IPR000394; RNA_pol_sigma_54.
DR InterPro; IPR007046; RNA_pol_sigma_54_core-bd.
DR InterPro; IPR007634; RNA_pol_sigma_54_DNA-bd.
DR InterPro; IPR038709; RpoN_core-bd_sf.
DR PANTHER; PTHR32248; PTHR32248; 1.
DR Pfam; PF00309; Sigma54_AID; 1.
DR Pfam; PF04963; Sigma54_CBD; 1.
DR Pfam; PF04552; Sigma54_DBD; 1.
DR PIRSF; PIRSF000774; RpoN; 1.
DR PRINTS; PR00045; SIGMA54FCT.
DR TIGRFAMs; TIGR02395; rpoN_sigma; 1.
DR PROSITE; PS00717; SIGMA54_1; 1.
DR PROSITE; PS00718; SIGMA54_2; 1.
DR PROSITE; PS50044; SIGMA54_3; 1.
PE 3: Inferred from homology;
KW DNA-binding; DNA-directed RNA polymerase; Nucleotidyltransferase;
KW Reference proteome; Sigma factor; Transcription; Transcription regulation;
KW Transferase.
FT CHAIN 1..519
FT /note="RNA polymerase sigma-54 factor"
FT /id="PRO_0000205540"
FT DNA_BIND 396..415
FT /note="H-T-H motif"
FT /evidence="ECO:0000255"
FT REGION 74..96
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 487..495
FT /note="RPON box"
FT CONFLICT 51..53
FT /note="FQS -> VPI (in Ref. 1; AAA26361)"
FT /evidence="ECO:0000305"
FT CONFLICT 58..71
FT /note="GFGSDRPERDETGL -> VSVRIGPSVMRHV (in Ref. 1;
FT AAA26361)"
FT /evidence="ECO:0000305"
FT CONFLICT 100..101
FT /note="ST -> RS (in Ref. 1; AAA26361)"
FT /evidence="ECO:0000305"
FT CONFLICT 120
FT /note="Missing (in Ref. 1; AAA26361)"
FT /evidence="ECO:0000305"
FT CONFLICT 149..155
FT /note="FVAGRKT -> SSPVGK (in Ref. 1; AAA26361)"
FT /evidence="ECO:0000305"
FT CONFLICT 171
FT /note="P -> A (in Ref. 1; AAA26361)"
FT /evidence="ECO:0000305"
FT CONFLICT 202
FT /note="G -> A (in Ref. 1; AAA26361)"
FT /evidence="ECO:0000305"
FT CONFLICT 213
FT /note="L -> V (in Ref. 1; AAA26361)"
FT /evidence="ECO:0000305"
FT CONFLICT 241
FT /note="R -> G (in Ref. 1; AAA26361)"
FT /evidence="ECO:0000305"
FT CONFLICT 257
FT /note="A -> G (in Ref. 1; AAA26361)"
FT /evidence="ECO:0000305"
FT CONFLICT 310
FT /note="A -> R (in Ref. 1; AAA26361)"
FT /evidence="ECO:0000305"
FT CONFLICT 422
FT /note="I -> R (in Ref. 1; AAA26361)"
FT /evidence="ECO:0000305"
FT CONFLICT 514..519
FT /note="AKAAGF -> PRQPASDCGFFAAAN (in Ref. 1; AAA26361)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 519 AA; 57196 MW; 9F46C913E0419375 CRC64;
MALSASLHLR QSQSLVMTPQ LMQSIQLLQM THLELGQFIA QEVEKNPLLE FQSADEAGFG
SDRPERDETG LTAEAGGAEE SVDHGDLYDS ATTSPGERLS TELDADFANV FQDDTAPQRA
DAPELLGQWK SMPGAGGSND GEGYDLDDFV AGRKTLRETL IEQVAFAFAA PADRLIAQHL
IDQLDEAGYL HAEIAETAAR LGASAADVTR VLLVLQQFDP PGVFARTLSE CLAIQLRLRN
RLDPAMEALV ANLELLARRD FASLKKICGV DEEDLIEMLA EIRKLDPKPG TSFETSVTEA
IIPDVVVRSA PDGGWLVELN PDALPRVLVN HDYFAEISRH SQKNSAEQAF LSECMQNANW
LTRSLDQRAR TILKVASEIV RQQDAFLVHG VDHLRPLNLR IVADAIKMHE STVSRVTSNK
YILTPRGLFE LKYFFTVSIG SAENGDAHSA ESVRHRIRTM ISQESADAVL SDDDIVDILK
RAGVDIARRT VAKYREAMNI PSSVQRRREK RALAKAAGF
