ID   RP55_BRADU              Reviewed;         537 AA.
AC   P30333;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1993, sequence version 1.
DT   03-AUG-2022, entry version 128.
DE   RecName: Full=RNA polymerase sigma-54 factor 2;
GN   Name=rpoN2; OrderedLocusNames=blr0723;
OS   Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 /
OS   NBRC 14792 / USDA 110).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Bradyrhizobiaceae; Bradyrhizobium.
OX   NCBI_TaxID=224911;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=USDA 110spc4;
RX   PubMed=1991712; DOI=10.1128/jb.173.3.1125-1138.1991;
RA   Kullik I., Fritsche S., Knobel H., Sanjuan J., Hennecke H., Fischer H.-M.;
RT   "Bradyrhizobium japonicum has two differentially regulated, functional
RT   homologs of the sigma 54 gene (rpoN).";
RL   J. Bacteriol. 173:1125-1138(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX   PubMed=12597275; DOI=10.1093/dnares/9.6.189;
RA   Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S.,
RA   Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M.,
RA   Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.;
RT   "Complete genomic sequence of nitrogen-fixing symbiotic bacterium
RT   Bradyrhizobium japonicum USDA110.";
RL   DNA Res. 9:189-197(2002).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. This sigma factor is responsible for the expression of the
CC       nitrogen fixation genes.
CC   -!- INDUCTION: Negatively autoregulated.
CC   -!- SIMILARITY: Belongs to the sigma-54 factor family. {ECO:0000305}.
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DR   EMBL; M59243; AAA26241.1; -; Genomic_DNA.
DR   EMBL; BA000040; BAC45988.1; -; Genomic_DNA.
DR   PIR; B38179; B38179.
DR   RefSeq; NP_767363.1; NC_004463.1.
DR   RefSeq; WP_011083548.1; NZ_CP011360.1.
DR   AlphaFoldDB; P30333; -.
DR   SMR; P30333; -.
DR   STRING; 224911.27348972; -.
DR   EnsemblBacteria; BAC45988; BAC45988; BAC45988.
DR   GeneID; 64020587; -.
DR   KEGG; bja:blr0723; -.
DR   PATRIC; fig|224911.44.peg.95; -.
DR   eggNOG; COG1508; Bacteria.
DR   HOGENOM; CLU_020569_0_0_5; -.
DR   InParanoid; P30333; -.
DR   OMA; VTTQKFM; -.
DR   PhylomeDB; P30333; -.
DR   Proteomes; UP000002526; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0001216; F:DNA-binding transcription activator activity; IEA:InterPro.
DR   GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR   GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.1330; -; 1.
DR   InterPro; IPR000394; RNA_pol_sigma_54.
DR   InterPro; IPR007046; RNA_pol_sigma_54_core-bd.
DR   InterPro; IPR007634; RNA_pol_sigma_54_DNA-bd.
DR   InterPro; IPR038709; RpoN_core-bd_sf.
DR   PANTHER; PTHR32248; PTHR32248; 1.
DR   Pfam; PF00309; Sigma54_AID; 1.
DR   Pfam; PF04963; Sigma54_CBD; 1.
DR   Pfam; PF04552; Sigma54_DBD; 1.
DR   PIRSF; PIRSF000774; RpoN; 1.
DR   PRINTS; PR00045; SIGMA54FCT.
DR   TIGRFAMs; TIGR02395; rpoN_sigma; 1.
DR   PROSITE; PS00717; SIGMA54_1; 1.
DR   PROSITE; PS00718; SIGMA54_2; 1.
DR   PROSITE; PS50044; SIGMA54_3; 1.
PE   2: Evidence at transcript level;
KW   DNA-binding; DNA-directed RNA polymerase; Nitrogen fixation;
KW   Nucleotidyltransferase; Reference proteome; Sigma factor; Transcription;
KW   Transcription regulation; Transferase.
FT   CHAIN           1..537
FT                   /note="RNA polymerase sigma-54 factor 2"
FT                   /id="PRO_0000205525"
FT   DNA_BIND        403..422
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255"
FT   REGION          52..90
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          507..537
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           492..500
FT                   /note="RPON box"
FT   COMPBIAS        512..531
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   537 AA;  58832 MW;  163D40BDAAE540EB CRC64;
     MALTQRLEFR QSQSLVMTPQ LMQAIKLLQL SNLDLTTFVE EELERNPLLE RANDEASGGE
     APAEAGQFSD SDGGHNDEPG GGPGEAFEPG QEEWMSKDLG TRAEIEQTLD TGLDNVFSEE
     PAEAAARNAQ DAAPTTYTEW GGGASGDEDY NLEAFVAAEV TLGDHLAEQL SVAFTAPAQR
     MIGQYLIDLV DEAGYLPPDL GQAAERLGAS QQEVEDVLAV LQKFDPPGVC ARNLSECLAI
     QLRELDRYDP AMQALVEHLD LLAKRDIAGL RKVCGVDDED IADMIGEIRR LNPKPGMKFG
     AARLQTMVPD VYVRPGPDGG WHVELNSDTL PRVLVNQTYY SELSKKIGKD GDKSYFTDAL
     QNATWLVRAL DQRARTILKV ATEIVRQQDG FFTHGVAHLR PLNLKAVADA IQMHESTVSR
     VTANKYMATN RGTFELKYFF TASIASADGG EAHSAEAVRH HIKQLIDSEA PAAILSDDTI
     VERLRASGID IARRTVAKYR EAMRIPSSVQ RRRDKQSALG NVLSTAMSDR SRNPEPA