ID   RPA1_EUPOC              Reviewed;         638 AA.
AC   P28363;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-1992, sequence version 1.
DT   03-AUG-2022, entry version 91.
DE   RecName: Full=DNA-directed RNA polymerase I subunit RPA1;
DE            EC=2.7.7.6;
DE   AltName: Full=DNA-directed RNA polymerase I largest subunit;
DE   Flags: Fragment;
GN   Name=RPA1;
OS   Euplotoides octocarinatus (Freshwater ciliate) (Euplotes octocarinatus).
OC   Eukaryota; Sar; Alveolata; Ciliophora; Intramacronucleata; Spirotrichea;
OC   Hypotrichia; Euplotida; Euplotidae; Euplotes.
OX   NCBI_TaxID=2716877;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=(68)-VIII;
RX   PubMed=1408746; DOI=10.1093/nar/20.17.4445;
RA   Kaufmann J., Klein A.;
RT   "Gene dosage as a possible major determinant for equal expression levels of
RT   genes encoding RNA polymerase subunits in the hypotrichous ciliate Euplotes
RT   octocarinatus.";
RL   Nucleic Acids Res. 20:4445-4450(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-94.
RX   PubMed=1461731; DOI=10.1093/nar/20.22.5985;
RA   Kaufmann J., Florian V., Klein A.;
RT   "TGA cysteine codons and intron sequences in conserved and nonconserved
RT   positions are found in macronuclear RNA polymerase genes of Euplotes
RT   octocarinatus.";
RL   Nucleic Acids Res. 20:5985-5989(1992).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       RNA polymerase I is essentially used to transcribe ribosomal DNA units.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6;
CC   -!- SUBUNIT: Each class of RNA polymerase is assembled from 9 to 14
CC       different polypeptides. This subunit is the largest component of RNA
CC       polymerase I.
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- MISCELLANEOUS: Three distinct zinc-containing RNA polymerases are found
CC       in eukaryotic nuclei: polymerase I for the ribosomal RNA precursor,
CC       polymerase II for the mRNA precursor, and polymerase III for 5S and
CC       tRNA genes.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family.
CC       {ECO:0000305}.
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DR   EMBL; X66450; CAA47066.1; -; Genomic_DNA.
DR   PIR; S33884; S33884.
DR   AlphaFoldDB; P28363; -.
DR   SMR; P28363; -.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 4.10.860.120; -; 1.
DR   InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR   InterPro; IPR000722; RNA_pol_asu.
DR   InterPro; IPR006592; RNA_pol_N.
DR   InterPro; IPR007080; RNA_pol_Rpb1_1.
DR   InterPro; IPR044893; RNA_pol_Rpb1_clamp_domain.
DR   PANTHER; PTHR19376; PTHR19376; 1.
DR   Pfam; PF04997; RNA_pol_Rpb1_1; 1.
DR   Pfam; PF00623; RNA_pol_Rpb1_2; 1.
DR   SMART; SM00663; RPOLA_N; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Metal-binding; Nucleotidyltransferase;
KW   Nucleus; Transcription; Transferase; Zinc.
FT   CHAIN           1..>638
FT                   /note="DNA-directed RNA polymerase I subunit RPA1"
FT                   /id="PRO_0000073927"
FT   REGION          297..321
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        297..312
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   NON_TER         638
SQ   SEQUENCE   638 AA;  73299 MW;  AB82F8BB9C22F5B5 CRC64;
     MATIFDSLIS SRLDGTRFSF YNDEEIQQMS VKEIHNPMAY DELNNPTSHG ICDESMGVSA
     LDKLSKCKTC GCDSVYCPGH MGHIKLTSTC YNPFTMKLLH SLLKSKCLVC HRLRISPKRI
     ELFEIRLKLI KLGYLVEAEK LSDFNHFSLE SIDSAIRILR KKTKGKTNKK EEKEDSEEVE
     EDKYAAKEAL ENLIQKEKEN REIFYSHISS ILQEDTPNES IGNAITVAIR DITKEIMNSV
     VPSKCPHCDV CNPRIKKDGA TKFFQMPLSN RDTKAMLSSH GRIDMRIDIS TMGAISEYDE
     EDDESSGESE VREGDEEQEK KQKYLSPIEV LEHMRRLWDV EDTLLNELFD NYKIFFMNNL
     LVSQNRFRPE SSGGKQSGDD RDYLHAHSAM LTKIINANIA FRRTIELKEN LTDDRNLETE
     SEKSKTQQID LSKTEITSKQ VIKAWAELQE SVNCYLDSSL AAKLENKEKP GLRQLLERKE
     GIFRMKMMGK RVNFAGRSVI SPDPYISTDQ IGVPEFIART ITFPEPAKNI EKLKRCVING
     AHKHPGANFI EYPNGERKAL ENMTLEEKKQ SSIIRKWRKI VYRHMQTGDP LLVNRQPTLH
     KPSIMAHNAI ILPKEQTIRM HYSNCKSYNA DFDGDEMK