ID   RPA43_DANRE             Reviewed;         391 AA.
AC   Q6PHG8; Q6DRH6;
DT   29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   29-MAY-2007, sequence version 2.
DT   03-AUG-2022, entry version 84.
DE   RecName: Full=DNA-directed RNA polymerase I subunit RPA43;
DE   AltName: Full=DNA-directed RNA polymerase I subunit F {ECO:0000250|UniProtKB:Q3B726};
DE   AltName: Full=Twist neighbor protein;
GN   Name=polr1f {ECO:0000250|UniProtKB:Q3B726}; Synonyms=twistnb;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX   PubMed=15256591; DOI=10.1073/pnas.0403929101;
RA   Amsterdam A., Nissen R.M., Sun Z., Swindell E.C., Farrington S.,
RA   Hopkins N.;
RT   "Identification of 315 genes essential for early zebrafish development.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:12792-12797(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 4-390.
RC   TISSUE=Kidney;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       Component of RNA polymerase I which synthesizes ribosomal RNA
CC       precursors. May be involved in recruitment of Pol I to rDNA promoters
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the RNA polymerase I (Pol I) complex consisting
CC       of at least 13 subunits. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the eukaryotic RPA43 RNA polymerase subunit
CC       family. {ECO:0000305}.
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DR   EMBL; AY648783; AAT68101.1; -; mRNA.
DR   EMBL; BC056557; AAH56557.1; -; mRNA.
DR   RefSeq; NP_001007154.1; NM_001007153.1.
DR   AlphaFoldDB; Q6PHG8; -.
DR   SMR; Q6PHG8; -.
DR   STRING; 7955.ENSDARP00000104602; -.
DR   PaxDb; Q6PHG8; -.
DR   GeneID; 402856; -.
DR   KEGG; dre:402856; -.
DR   CTD; 221830; -.
DR   ZFIN; ZDB-GENE-041007-3; polr1f.
DR   eggNOG; KOG4134; Eukaryota.
DR   InParanoid; Q6PHG8; -.
DR   OrthoDB; 1196232at2759; -.
DR   Reactome; R-DRE-5250924; B-WICH complex positively regulates rRNA expression.
DR   Reactome; R-DRE-73762; RNA Polymerase I Transcription Initiation.
DR   Reactome; R-DRE-73772; RNA Polymerase I Promoter Escape.
DR   Reactome; R-DRE-73863; RNA Polymerase I Transcription Termination.
DR   PRO; PR:Q6PHG8; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0005736; C:RNA polymerase I complex; IBA:GO_Central.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR   CDD; cd04328; RNAP_I_Rpa43_N; 1.
DR   Gene3D; 3.30.1490.120; -; 1.
DR   InterPro; IPR036898; RNA_pol_Rpb7-like_N_sf.
DR   InterPro; IPR041901; RNAP_I_Rpa43_N.
DR   InterPro; IPR045113; Rpb7-like.
DR   InterPro; IPR005576; Rpb7-like_N.
DR   PANTHER; PTHR12709; PTHR12709; 1.
DR   Pfam; PF03876; SHS2_Rpb7-N; 1.
PE   2: Evidence at transcript level;
KW   Coiled coil; DNA-directed RNA polymerase; Nucleus; Phosphoprotein;
KW   Reference proteome; Transcription.
FT   CHAIN           1..391
FT                   /note="DNA-directed RNA polymerase I subunit RPA43"
FT                   /id="PRO_0000288634"
FT   REGION          1..27
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          220..391
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          289..353
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        260..274
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        275..289
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        308..325
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        364..385
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        239
FT                   /note="D -> G (in Ref. 1; AAT68101)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        292
FT                   /note="E -> EK (in Ref. 1; AAT68101)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        338..339
FT                   /note="Missing (in Ref. 1; AAT68101)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        379
FT                   /note="T -> I (in Ref. 1; AAT68101)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        382
FT                   /note="P -> L (in Ref. 1; AAT68101)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   391 AA;  43286 MW;  8DB4E9FA05C7B893 CRC64;
     MANWTQEDGA PTPVTNPSEV SQVSGGSVTG GPAVTSCLIP SFAEAVKLLK ARYSCLVLDT
     HRRHISLPPV HLKKKKTGIQ EQLNAELLKY SNSLDGVPVA YDNIKVVGQH GNIYDDQGFI
     HFNIEASFVI FRPKNGSRLM GVINKMGASH VGCLVHGCFN ASVMKPNALT SDQWRDSGLC
     VGQSLEFEVF QLDADAAGVL LIRGRLDRSR VQELVAQFEQ KQVTAESSTE ADATEDTTDS
     PKPKKKKKRK KDKNDTESSM EECVNNSSLQ ETSEHHQTTT EEDCSANGRH KEKKKKKKRD
     KNDTESSMDE CMNNNSLQET ALDTTEEDCN ANERHKEKKK KKKRDKQQDS AEIVPTSDSS
     GYISDKTSRK RALEAGDDTE TPAAKKKKKS K