RPA43_MOUSE
ID RPA43_MOUSE Reviewed; 330 AA.
AC Q78WZ7; Q3U6L1; Q8CEP7; Q9CS60;
DT 29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=DNA-directed RNA polymerase I subunit RPA43;
DE AltName: Full=DNA-directed RNA polymerase I subunit F {ECO:0000250|UniProtKB:Q3B726};
DE AltName: Full=Twist neighbor protein {ECO:0000303|PubMed:12438708};
GN Name=Polr1f {ECO:0000250|UniProtKB:Q3B726};
GN Synonyms=Twistnb {ECO:0000303|PubMed:12438708};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Bone marrow, and Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP TISSUE SPECIFICITY.
RX PubMed=12438708; DOI=10.1159/000066618;
RA Kosan C., Kunz J.;
RT "Identification and characterisation of the gene TWIST NEIGHBOR (TWISTNB)
RT located in the microdeletion syndrome 7p21 region.";
RL Cytogenet. Genome Res. 97:167-170(2002).
RN [3]
RP INTERACTION WITH RRN3.
RX PubMed=12393749; DOI=10.1093/embo-reports/kvf212;
RA Yuan X., Zhao J., Zentgraf H., Hoffmann-Rohrer U., Grummt I.;
RT "Multiple interactions between RNA polymerase I, TIF-IA and TAF(I) subunits
RT regulate preinitiation complex assembly at the ribosomal gene promoter.";
RL EMBO Rep. 3:1082-1087(2002).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC Component of RNA polymerase I which synthesizes ribosomal RNA
CC precursors. Through its association with RRN3/TIF-IA may be involved in
CC recruitment of Pol I to rDNA promoters.
CC -!- SUBUNIT: Component of the RNA polymerase I (Pol I) complex consisting
CC of at least 13 subunits (By similarity). Interacts with RRN3/TIF-IA.
CC {ECO:0000250, ECO:0000269|PubMed:12393749}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Widely expressed. {ECO:0000269|PubMed:12438708}.
CC -!- SIMILARITY: Belongs to the eukaryotic RPA43 RNA polymerase subunit
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC25502.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AK010829; BAB27210.1; -; mRNA.
DR EMBL; AK017810; BAB30947.1; -; mRNA.
DR EMBL; AK017016; BAC25502.1; ALT_FRAME; mRNA.
DR EMBL; AK153090; BAE31713.1; -; mRNA.
DR CCDS; CCDS25877.1; -.
DR RefSeq; NP_758457.1; NM_172253.2.
DR AlphaFoldDB; Q78WZ7; -.
DR SMR; Q78WZ7; -.
DR BioGRID; 205748; 1.
DR STRING; 10090.ENSMUSP00000020877; -.
DR iPTMnet; Q78WZ7; -.
DR PhosphoSitePlus; Q78WZ7; -.
DR EPD; Q78WZ7; -.
DR jPOST; Q78WZ7; -.
DR MaxQB; Q78WZ7; -.
DR PaxDb; Q78WZ7; -.
DR PRIDE; Q78WZ7; -.
DR ProteomicsDB; 262699; -.
DR Antibodypedia; 11907; 96 antibodies from 21 providers.
DR DNASU; 28071; -.
DR Ensembl; ENSMUST00000020877; ENSMUSP00000020877; ENSMUSG00000020561.
DR GeneID; 28071; -.
DR KEGG; mmu:28071; -.
DR UCSC; uc007niu.1; mouse.
DR CTD; 221830; -.
DR MGI; MGI:106292; Polr1f.
DR VEuPathDB; HostDB:ENSMUSG00000020561; -.
DR eggNOG; KOG4134; Eukaryota.
DR GeneTree; ENSGT00390000005553; -.
DR HOGENOM; CLU_048289_0_0_1; -.
DR InParanoid; Q78WZ7; -.
DR OMA; HYSCLVA; -.
DR OrthoDB; 1196232at2759; -.
DR PhylomeDB; Q78WZ7; -.
DR TreeFam; TF325602; -.
DR Reactome; R-MMU-5250924; B-WICH complex positively regulates rRNA expression.
DR Reactome; R-MMU-73762; RNA Polymerase I Transcription Initiation.
DR Reactome; R-MMU-73772; RNA Polymerase I Promoter Escape.
DR Reactome; R-MMU-73863; RNA Polymerase I Transcription Termination.
DR BioGRID-ORCS; 28071; 26 hits in 73 CRISPR screens.
DR PRO; PR:Q78WZ7; -.
DR Proteomes; UP000000589; Chromosome 12.
DR RNAct; Q78WZ7; protein.
DR Bgee; ENSMUSG00000020561; Expressed in manus and 228 other tissues.
DR ExpressionAtlas; Q78WZ7; baseline and differential.
DR Genevisible; Q78WZ7; MM.
DR GO; GO:0005736; C:RNA polymerase I complex; IBA:GO_Central.
DR GO; GO:1990830; P:cellular response to leukemia inhibitory factor; IEP:MGI.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR CDD; cd04328; RNAP_I_Rpa43_N; 1.
DR Gene3D; 3.30.1490.120; -; 1.
DR InterPro; IPR036898; RNA_pol_Rpb7-like_N_sf.
DR InterPro; IPR041901; RNAP_I_Rpa43_N.
DR InterPro; IPR045113; Rpb7-like.
DR PANTHER; PTHR12709; PTHR12709; 1.
PE 1: Evidence at protein level;
KW DNA-directed RNA polymerase; Nucleus; Phosphoprotein; Reference proteome;
KW Transcription.
FT CHAIN 1..330
FT /note="DNA-directed RNA polymerase I subunit RPA43"
FT /id="PRO_0000288633"
FT REGION 251..330
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 251..266
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 306
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q3B726"
FT MOD_RES 318
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q3B726"
FT MOD_RES 325
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q3B726"
FT CONFLICT 124
FT /note="C -> S (in Ref. 1; BAE31713)"
FT /evidence="ECO:0000305"
FT CONFLICT 134
FT /note="T -> I (in Ref. 1; BAC25502)"
FT /evidence="ECO:0000305"
FT CONFLICT 225
FT /note="K -> N (in Ref. 1; BAC25502)"
FT /evidence="ECO:0000305"
FT CONFLICT 259
FT /note="Q -> R (in Ref. 1; BAC25502)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 330 AA; 36721 MW; C68996895950DA73 CRC64;
MAAGSVESQR SQAASERPVA GQAGVLPCLE LPSYAAACAL VGSRYSCLVA APHRRHIALS
PRYLSRKRTG IREQLDAELL RYSESLLGVP IAYDNIRVVG ELGDIYDDQG HIHLNIEADF
VIFCPEPGQT LMGTVNKVSS SHIGCLVHGC FNASIPKPEQ MSYEEWQTLE IHVGDELEFD
VFRLDSDSAG VFCIRGKLST TSLQLKHSAV SEDVAETVVE EVVEKTPKKK KKKKDKDTDT
CGTVDSVTEV ADVTDVTPQE ETDIPCSDNV NDFFEEEPKK KKKKKKRHQE DQDPIFQASD
SSGYQSDHNK KKKKRKHSEE ANFESPKKRQ
