RPA43_SCHPO
ID RPA43_SCHPO Reviewed; 173 AA.
AC O43036;
DT 06-DEC-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=DNA-directed RNA polymerase I subunit rpa43;
DE Short=RNA polymerase I subunit A43;
DE AltName: Full=DNA-dependent RNA polymerase 19 kDa polypeptide;
GN Name=rpa43; Synonyms=rpa21; ORFNames=SPBC3B9.07c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=10645483;
RA Shpakovskii G.V., Shematorova E.K.;
RT "Characteristics of the cDNA of the Schizosaccharomyces pombe rpa43+ gene:
RT structural similarity of the Rpa43 subunit of RNA-polymerase I with the
RT Rpc25 subunit of RNA-polymerase III.";
RL Bioorg. Khim. 25:791-796(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [3]
RP INTERACTION WITH KER1.
RC STRAIN=972 / ATCC 24843;
RX PubMed=15647272; DOI=10.1074/jbc.m411150200;
RA Imazawa Y., Hisatake K., Mitsuzawa H., Matsumoto M., Tsukui T.,
RA Nakagawa K., Nakadai T., Shimada M., Ishihama A., Nogi Y.;
RT "The fission yeast protein Ker1p is an ortholog of RNA polymerase I subunit
RT A14 in Saccharomyces cerevisiae and is required for stable association of
RT Rrn3p and RPA21 in RNA polymerase I.";
RL J. Biol. Chem. 280:11467-11474(2005).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC Component of RNA polymerase I which synthesizes ribosomal RNA
CC precursors. May be involved in recruitment of Pol I to rDNA promoters.
CC -!- SUBUNIT: Component of the RNA polymerase I (Pol I) complex consisting
CC of 14 subunits. Part of a Pol I subcomplex consisting of the subunits
CC A14 and A43 (By similarity). Interacts with ker1/A14. {ECO:0000250,
CC ECO:0000269|PubMed:15647272}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}.
CC -!- PTM: Contains an average of four phosphates per molecule.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the eukaryotic RPA43 RNA polymerase subunit
CC family. {ECO:0000305}.
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DR EMBL; AF129106; AAF40067.1; -; mRNA.
DR EMBL; CU329671; CAA17787.1; -; Genomic_DNA.
DR PIR; T40346; T40346.
DR RefSeq; NP_596665.1; NM_001022587.2.
DR PDB; 7AOC; EM; 3.84 A; G=1-173.
DR PDB; 7AOD; EM; 4.50 A; G/S=1-173.
DR PDB; 7AOE; EM; 3.90 A; G=1-173.
DR PDBsum; 7AOC; -.
DR PDBsum; 7AOD; -.
DR PDBsum; 7AOE; -.
DR AlphaFoldDB; O43036; -.
DR SMR; O43036; -.
DR BioGRID; 277505; 5.
DR STRING; 4896.SPBC3B9.07c.1; -.
DR MaxQB; O43036; -.
DR PaxDb; O43036; -.
DR EnsemblFungi; SPBC3B9.07c.1; SPBC3B9.07c.1:pep; SPBC3B9.07c.
DR GeneID; 2540989; -.
DR KEGG; spo:SPBC3B9.07c; -.
DR PomBase; SPBC3B9.07c; rpa43.
DR VEuPathDB; FungiDB:SPBC3B9.07c; -.
DR eggNOG; KOG4134; Eukaryota.
DR HOGENOM; CLU_105516_0_0_1; -.
DR InParanoid; O43036; -.
DR OMA; SKMPRIN; -.
DR PhylomeDB; O43036; -.
DR Reactome; R-SPO-73762; RNA Polymerase I Transcription Initiation.
DR Reactome; R-SPO-73772; RNA Polymerase I Promoter Escape.
DR PRO; PR:O43036; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0005730; C:nucleolus; HDA:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0005736; C:RNA polymerase I complex; ISO:PomBase.
DR GO; GO:0006361; P:transcription initiation from RNA polymerase I promoter; IMP:PomBase.
DR Gene3D; 3.30.1490.120; -; 1.
DR InterPro; IPR036898; RNA_pol_Rpb7-like_N_sf.
DR InterPro; IPR041178; RPA43_OB.
DR InterPro; IPR045113; Rpb7-like.
DR PANTHER; PTHR12709; PTHR12709; 1.
DR Pfam; PF17875; RPA43_OB; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-directed RNA polymerase; Nucleus; Phosphoprotein;
KW Reference proteome; Transcription.
FT CHAIN 1..173
FT /note="DNA-directed RNA polymerase I subunit rpa43"
FT /id="PRO_0000073959"
SQ SEQUENCE 173 AA; 19385 MW; 01AD7375E3092674 CRC64;
MPDLSLYKQT VDLYLSIAPG HSRDPLNAIQ EHMDSMILSK LPRINGIVLA YDNIRFLEKS
AKVMYDSPFS FIWVRVDVLV FSPKKGDCLE GKINLVSPSH IGLLILGIFN ASIPRKSIPK
DWIFIEPDTT EEQGRWKTND GNILEPGKDL EFVVDGIQRE AGLTMVQGTL ANS
