RPAB1_MOUSE
ID RPAB1_MOUSE Reviewed; 210 AA.
AC Q80UW8;
DT 11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=DNA-directed RNA polymerases I, II, and III subunit RPABC1;
DE Short=RNA polymerases I, II, and III subunit ABC1;
DE AltName: Full=DNA-directed RNA polymerase II subunit E;
DE AltName: Full=RPB5 homolog;
GN Name=Polr2e;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney, Lung, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC Common component of RNA polymerases I, II and III which synthesize
CC ribosomal RNA precursors, mRNA precursors and many functional non-
CC coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively.
CC Pol II is the central component of the basal RNA polymerase II
CC transcription machinery. Pols are composed of mobile elements that move
CC relative to each other. In Pol II, POLR2E/RPB5 is part of the lower jaw
CC surrounding the central large cleft and thought to grab the incoming
CC DNA template. Seems to be the major component in this process (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the RNA polymerase I (Pol I), RNA polymerase II
CC (Pol II) and RNA polymerase III (Pol III) complexes consisting of at
CC least 13, 12 and 17 subunits, respectively. In RNA Pol II, this subunit
CC is present in 2-fold molar excess over the other subunits. Component of
CC the PAQosome complex which is responsible for the biogenesis of several
CC protein complexes and which consists of R2TP complex members RUVBL1,
CC RUVBL2, RPAP3 and PIH1D1, URI complex members PFDN2, PFDN6, PDRG1, UXT
CC and URI1 as well as ASDURF, POLR2E and DNAAF10/WDR92. Interacts with
CC URI1. {ECO:0000250|UniProtKB:P19388}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the archaeal Rpo5/eukaryotic RPB5 RNA polymerase
CC subunit family. {ECO:0000305}.
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DR EMBL; BC045521; AAH45521.1; -; mRNA.
DR CCDS; CCDS35972.1; -.
DR RefSeq; NP_079830.2; NM_025554.2.
DR AlphaFoldDB; Q80UW8; -.
DR SMR; Q80UW8; -.
DR BioGRID; 211461; 32.
DR IntAct; Q80UW8; 6.
DR MINT; Q80UW8; -.
DR STRING; 10090.ENSMUSP00000004786; -.
DR iPTMnet; Q80UW8; -.
DR PhosphoSitePlus; Q80UW8; -.
DR EPD; Q80UW8; -.
DR MaxQB; Q80UW8; -.
DR PaxDb; Q80UW8; -.
DR PRIDE; Q80UW8; -.
DR ProteomicsDB; 300469; -.
DR Antibodypedia; 22550; 316 antibodies from 28 providers.
DR DNASU; 66420; -.
DR Ensembl; ENSMUST00000004786; ENSMUSP00000004786; ENSMUSG00000004667.
DR GeneID; 66420; -.
DR KEGG; mmu:66420; -.
DR UCSC; uc007gbi.1; mouse.
DR CTD; 5434; -.
DR MGI; MGI:1913670; Polr2e.
DR VEuPathDB; HostDB:ENSMUSG00000004667; -.
DR eggNOG; KOG3218; Eukaryota.
DR GeneTree; ENSGT00390000013841; -.
DR HOGENOM; CLU_058320_0_1_1; -.
DR InParanoid; Q80UW8; -.
DR OMA; VRDRGYF; -.
DR OrthoDB; 1255823at2759; -.
DR PhylomeDB; Q80UW8; -.
DR TreeFam; TF103040; -.
DR Reactome; R-MMU-112382; Formation of RNA Pol II elongation complex.
DR Reactome; R-MMU-113418; Formation of the Early Elongation Complex.
DR Reactome; R-MMU-5250924; B-WICH complex positively regulates rRNA expression.
DR Reactome; R-MMU-5578749; Transcriptional regulation by small RNAs.
DR Reactome; R-MMU-674695; RNA Polymerase II Pre-transcription Events.
DR Reactome; R-MMU-6781823; Formation of TC-NER Pre-Incision Complex.
DR Reactome; R-MMU-6782135; Dual incision in TC-NER.
DR Reactome; R-MMU-6782210; Gap-filling DNA repair synthesis and ligation in TC-NER.
DR Reactome; R-MMU-6796648; TP53 Regulates Transcription of DNA Repair Genes.
DR Reactome; R-MMU-6803529; FGFR2 alternative splicing.
DR Reactome; R-MMU-6807505; RNA polymerase II transcribes snRNA genes.
DR Reactome; R-MMU-72086; mRNA Capping.
DR Reactome; R-MMU-72163; mRNA Splicing - Major Pathway.
DR Reactome; R-MMU-72165; mRNA Splicing - Minor Pathway.
DR Reactome; R-MMU-72203; Processing of Capped Intron-Containing Pre-mRNA.
DR Reactome; R-MMU-73762; RNA Polymerase I Transcription Initiation.
DR Reactome; R-MMU-73772; RNA Polymerase I Promoter Escape.
DR Reactome; R-MMU-73776; RNA Polymerase II Promoter Escape.
DR Reactome; R-MMU-73779; RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
DR Reactome; R-MMU-73863; RNA Polymerase I Transcription Termination.
DR Reactome; R-MMU-75953; RNA Polymerase II Transcription Initiation.
DR Reactome; R-MMU-75955; RNA Polymerase II Transcription Elongation.
DR Reactome; R-MMU-76042; RNA Polymerase II Transcription Initiation And Promoter Clearance.
DR Reactome; R-MMU-76061; RNA Polymerase III Transcription Initiation From Type 1 Promoter.
DR Reactome; R-MMU-76066; RNA Polymerase III Transcription Initiation From Type 2 Promoter.
DR Reactome; R-MMU-76071; RNA Polymerase III Transcription Initiation From Type 3 Promoter.
DR Reactome; R-MMU-77075; RNA Pol II CTD phosphorylation and interaction with CE.
DR Reactome; R-MMU-9018519; Estrogen-dependent gene expression.
DR BioGRID-ORCS; 66420; 23 hits in 72 CRISPR screens.
DR PRO; PR:Q80UW8; -.
DR Proteomes; UP000000589; Chromosome 10.
DR RNAct; Q80UW8; protein.
DR Bgee; ENSMUSG00000004667; Expressed in yolk sac and 266 other tissues.
DR ExpressionAtlas; Q80UW8; baseline and differential.
DR Genevisible; Q80UW8; MM.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0005736; C:RNA polymerase I complex; IBA:GO_Central.
DR GO; GO:0005665; C:RNA polymerase II, core complex; ISS:UniProtKB.
DR GO; GO:0005666; C:RNA polymerase III complex; IBA:GO_Central.
DR GO; GO:1990062; C:RPAP3/R2TP/prefoldin-like complex; ISO:MGI.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:InterPro.
DR GO; GO:0006366; P:transcription by RNA polymerase II; ISS:UniProtKB.
DR Gene3D; 3.40.1340.10; -; 1.
DR Gene3D; 3.90.940.20; -; 1.
DR HAMAP; MF_00025; RNApol_Rpo5_RPB5; 1.
DR InterPro; IPR014381; Arch_Rpo5/euc_Rpb5.
DR InterPro; IPR005571; RNA_pol_Rpb5_N.
DR InterPro; IPR036710; RNA_pol_Rpb5_N_sf.
DR InterPro; IPR000783; RNA_pol_subH/Rpb5_C.
DR InterPro; IPR020608; RNA_pol_subH/Rpb5_CS.
DR InterPro; IPR035913; RPB5-like_sf.
DR PANTHER; PTHR10535; PTHR10535; 1.
DR Pfam; PF01191; RNA_pol_Rpb5_C; 1.
DR Pfam; PF03871; RNA_pol_Rpb5_N; 1.
DR PIRSF; PIRSF000747; RPB5; 1.
DR SUPFAM; SSF53036; SSF53036; 1.
DR SUPFAM; SSF55287; SSF55287; 1.
DR PROSITE; PS01110; RNA_POL_H_23KD; 1.
PE 1: Evidence at protein level;
KW Acetylation; DNA-directed RNA polymerase; Isopeptide bond; Nucleus;
KW Reference proteome; Transcription; Ubl conjugation.
FT CHAIN 1..210
FT /note="DNA-directed RNA polymerases I, II, and III subunit
FT RPABC1"
FT /id="PRO_0000146076"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:P19388"
FT CROSSLNK 81
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:P19388"
SQ SEQUENCE 210 AA; 24570 MW; E09DEB40E05F3554 CRC64;
MDDEEETYRL WKIRKTIMQL CHDRGYLVTQ DELDQTLEEF KAQFGDKPSE GRPRRTDLTV
LVAHNDDPTD QMFVFFPEEP KVGIKTIKVY CQRMQEENIT RALIVVQQGM TPSAKQSLVD
MAPKYVLEQF LQQELLINIT EHELVPEHVV MTKEEVTELL ARYKLRESQL PRIQAGDPVA
RYFGIKRGQV VKIIRPSETA GRYITYRLVQ
