ID   RPAB1_PONAB             Reviewed;         210 AA.
AC   Q5R587;
DT   13-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT   21-DEC-2004, sequence version 1.
DT   03-AUG-2022, entry version 75.
DE   RecName: Full=DNA-directed RNA polymerases I, II, and III subunit RPABC1;
DE            Short=RNA polymerases I, II, and III subunit ABC1;
DE   AltName: Full=DNA-directed RNA polymerase II subunit E;
DE   AltName: Full=RPB5 homolog;
GN   Name=POLR2E;
OS   Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Pongo.
OX   NCBI_TaxID=9601;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain cortex;
RG   The German cDNA consortium;
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       Common component of RNA polymerases I, II and III which synthesize
CC       ribosomal RNA precursors, mRNA precursors and many functional non-
CC       coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively.
CC       Pol II is the central component of the basal RNA polymerase II
CC       transcription machinery. Pols are composed of mobile elements that move
CC       relative to each other. In Pol II, POLR2E/RPB5 is part of the lower jaw
CC       surrounding the central large cleft and thought to grab the incoming
CC       DNA template. Seems to be the major component in this process (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the RNA polymerase I (Pol I), RNA polymerase II
CC       (Pol II) and RNA polymerase III (Pol III) complexes consisting of at
CC       least 13, 12 and 17 subunits, respectively. In RNA Pol II, this subunit
CC       is present in 2-fold molar excess over the other subunits. Component of
CC       the PAQosome complex which is responsible for the biogenesis of several
CC       protein complexes and which consists of R2TP complex members RUVBL1,
CC       RUVBL2, RPAP3 and PIH1D1, URI complex members PFDN2, PFDN6, PDRG1, UXT
CC       and URI1 as well as ASDURF, POLR2E and DNAAF10/WDR92. Interacts with
CC       URI1. {ECO:0000250|UniProtKB:P19388}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the archaeal Rpo5/eukaryotic RPB5 RNA polymerase
CC       subunit family. {ECO:0000305}.
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DR   EMBL; CR860977; CAH93079.1; -; mRNA.
DR   RefSeq; NP_001126823.1; NM_001133351.1.
DR   AlphaFoldDB; Q5R587; -.
DR   SMR; Q5R587; -.
DR   STRING; 9601.ENSPPYP00000010442; -.
DR   GeneID; 100173829; -.
DR   KEGG; pon:100173829; -.
DR   CTD; 5434; -.
DR   eggNOG; KOG3218; Eukaryota.
DR   InParanoid; Q5R587; -.
DR   OrthoDB; 1255823at2759; -.
DR   Proteomes; UP000001595; Unplaced.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0005665; C:RNA polymerase II, core complex; ISS:UniProtKB.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:InterPro.
DR   GO; GO:0006366; P:transcription by RNA polymerase II; ISS:UniProtKB.
DR   Gene3D; 3.40.1340.10; -; 1.
DR   Gene3D; 3.90.940.20; -; 1.
DR   HAMAP; MF_00025; RNApol_Rpo5_RPB5; 1.
DR   InterPro; IPR014381; Arch_Rpo5/euc_Rpb5.
DR   InterPro; IPR005571; RNA_pol_Rpb5_N.
DR   InterPro; IPR036710; RNA_pol_Rpb5_N_sf.
DR   InterPro; IPR000783; RNA_pol_subH/Rpb5_C.
DR   InterPro; IPR020608; RNA_pol_subH/Rpb5_CS.
DR   InterPro; IPR035913; RPB5-like_sf.
DR   PANTHER; PTHR10535; PTHR10535; 1.
DR   Pfam; PF01191; RNA_pol_Rpb5_C; 1.
DR   Pfam; PF03871; RNA_pol_Rpb5_N; 1.
DR   PIRSF; PIRSF000747; RPB5; 1.
DR   SUPFAM; SSF53036; SSF53036; 1.
DR   SUPFAM; SSF55287; SSF55287; 1.
DR   PROSITE; PS01110; RNA_POL_H_23KD; 1.
PE   2: Evidence at transcript level;
KW   Acetylation; DNA-directed RNA polymerase; Isopeptide bond; Nucleus;
KW   Reference proteome; Transcription; Ubl conjugation.
FT   CHAIN           1..210
FT                   /note="DNA-directed RNA polymerases I, II, and III subunit
FT                   RPABC1"
FT                   /id="PRO_0000146077"
FT   MOD_RES         1
FT                   /note="N-acetylmethionine"
FT                   /evidence="ECO:0000250|UniProtKB:P19388"
FT   CROSSLNK        81
FT                   /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT                   G-Cter in SUMO2)"
FT                   /evidence="ECO:0000250|UniProtKB:P19388"
SQ   SEQUENCE   210 AA;  24639 MW;  123885AE3450648C CRC64;
     MDDEEETYRL WKIRKTIMQL CHDRGYLVTQ DELDQTLEEF RAQFGDKPSE GRPRRTDLTV
     LVAHNDDPTD QMFVFFPEEP KVGIKTIKVY CQRMQEENIT RALIVVQQGM TPSAKQSLVD
     MAPKYILEQF LQQELLINIT EHELVPEHVV MTKEEVTELL ARYKLRENQL PRIQAGDPVA
     RYFGIKRGQV VKIIRPSETA GRYITYRLVQ