RPAB3_DICDI
ID RPAB3_DICDI Reviewed; 142 AA.
AC Q54YW8;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 25-MAY-2022, entry version 100.
DE RecName: Full=DNA-directed RNA polymerases I, II, and III subunit rpabc3;
DE Short=RNA polymerases I, II, and III subunit ABC3;
DE AltName: Full=RNA polymerase B subunit 8;
DE Short=RPB8;
DE AltName: Full=RPB17;
GN Name=polr2h; Synonyms=rpb8; ORFNames=DDB_G0278039;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC Common component of RNA polymerases I, II and III which synthesize
CC ribosomal RNA precursors, mRNA precursors and many functional non-
CC coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively
CC (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the RNA polymerase I (Pol I), RNA polymerase II
CC (Pol II) and RNA polymerase III (Pol III) complexes consisting of at
CC least 13, 12 and 17 subunits, respectively. Directly interacts with
CC POLR2A (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the eukaryotic RPB8 RNA polymerase subunit
CC family. {ECO:0000305}.
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DR EMBL; AAFI02000023; EAL68192.1; -; Genomic_DNA.
DR RefSeq; XP_642084.1; XM_636992.1.
DR AlphaFoldDB; Q54YW8; -.
DR SMR; Q54YW8; -.
DR STRING; 44689.DDB0216297; -.
DR PaxDb; Q54YW8; -.
DR EnsemblProtists; EAL68192; EAL68192; DDB_G0278039.
DR GeneID; 8621297; -.
DR KEGG; ddi:DDB_G0278039; -.
DR dictyBase; DDB_G0278039; rpb8.
DR eggNOG; KOG3400; Eukaryota.
DR HOGENOM; CLU_103864_1_1_1; -.
DR InParanoid; Q54YW8; -.
DR OMA; TINAYVS; -.
DR PhylomeDB; Q54YW8; -.
DR Reactome; R-DDI-113418; Formation of the Early Elongation Complex.
DR Reactome; R-DDI-674695; RNA Polymerase II Pre-transcription Events.
DR Reactome; R-DDI-6781823; Formation of TC-NER Pre-Incision Complex.
DR Reactome; R-DDI-6782135; Dual incision in TC-NER.
DR Reactome; R-DDI-6782210; Gap-filling DNA repair synthesis and ligation in TC-NER.
DR Reactome; R-DDI-6796648; TP53 Regulates Transcription of DNA Repair Genes.
DR Reactome; R-DDI-6803529; FGFR2 alternative splicing.
DR Reactome; R-DDI-6807505; RNA polymerase II transcribes snRNA genes.
DR Reactome; R-DDI-72086; mRNA Capping.
DR Reactome; R-DDI-72163; mRNA Splicing - Major Pathway.
DR Reactome; R-DDI-72165; mRNA Splicing - Minor Pathway.
DR Reactome; R-DDI-73762; RNA Polymerase I Transcription Initiation.
DR Reactome; R-DDI-73772; RNA Polymerase I Promoter Escape.
DR Reactome; R-DDI-73776; RNA Polymerase II Promoter Escape.
DR Reactome; R-DDI-73779; RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
DR Reactome; R-DDI-75953; RNA Polymerase II Transcription Initiation.
DR Reactome; R-DDI-75955; RNA Polymerase II Transcription Elongation.
DR Reactome; R-DDI-76042; RNA Polymerase II Transcription Initiation And Promoter Clearance.
DR Reactome; R-DDI-76061; RNA Polymerase III Transcription Initiation From Type 1 Promoter.
DR Reactome; R-DDI-76066; RNA Polymerase III Transcription Initiation From Type 2 Promoter.
DR Reactome; R-DDI-77075; RNA Pol II CTD phosphorylation and interaction with CE.
DR Reactome; R-DDI-9018519; Estrogen-dependent gene expression.
DR PRO; PR:Q54YW8; -.
DR Proteomes; UP000002195; Chromosome 3.
DR GO; GO:0005736; C:RNA polymerase I complex; ISS:dictyBase.
DR GO; GO:0005665; C:RNA polymerase II, core complex; ISS:dictyBase.
DR GO; GO:0005666; C:RNA polymerase III complex; ISS:dictyBase.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; ISS:dictyBase.
DR GO; GO:0006360; P:transcription by RNA polymerase I; ISS:dictyBase.
DR GO; GO:0006366; P:transcription by RNA polymerase II; ISS:dictyBase.
DR GO; GO:0006383; P:transcription by RNA polymerase III; ISS:dictyBase.
DR Gene3D; 2.40.50.140; -; 1.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR005570; RNA_pol_Rpb8.
DR PANTHER; PTHR10917; PTHR10917; 1.
DR Pfam; PF03870; RNA_pol_Rpb8; 1.
DR PIRSF; PIRSF000779; RNA_pol_Rpb8; 1.
DR SMART; SM00658; RPOL8c; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
PE 3: Inferred from homology;
KW DNA-binding; DNA-directed RNA polymerase; Nucleus; Reference proteome;
KW Transcription.
FT CHAIN 1..142
FT /note="DNA-directed RNA polymerases I, II, and III subunit
FT rpabc3"
FT /id="PRO_0000328221"
FT REGION 16..40
FT /note="Non-specific ssDNA binding"
FT /evidence="ECO:0000250"
SQ SEQUENCE 142 AA; 16177 MW; 6ED60DFB3EAC80FF CRC64;
MTSILFEDTF EVKEIDPDGK KFDRVSRFVC YSENYEMDLQ IDIATHIYPL GHERFKMVLA
SSLNLDGSSD NSGSYDTNKP SLADKYDYVM YGKIFKYQKE NSSSKVSVFA SFGGLLVLLQ
GDPRYLPGIE LDARIYLLIK KA
