RPAB3_SCHPO
ID RPAB3_SCHPO Reviewed; 125 AA.
AC Q92399; O13459; O13460;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 15-JUL-1998, sequence version 2.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=DNA-directed RNA polymerases I, II, and III subunit RPABC3;
DE Short=RNA polymerases I, II, and III subunit ABC3;
DE AltName: Full=DNA-directed RNA polymerases I, II, and III 14.5 kDa polypeptide;
DE AltName: Full=RPC14;
GN Name=rpb8; ORFNames=SPBC14C8.12;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=9322754; DOI=10.1016/s0378-1119(97)00222-9;
RA Sakurai H., Ishihama A.;
RT "Gene organization and protein sequence of the small subunits of
RT Schizosaccharomyces pombe RNA polymerase II.";
RL Gene 196:165-174(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=972 / ATCC 24843;
RA Shpakovski G.V., Lebedenko E.N.;
RT "Structural and functional studies of the subunits shared by nuclear RNA
RT polymerases I, II and III of the fission yeast Schizosaccharomyces pombe.";
RL Tsitologiia 39:122-123(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RA Shpakovski G.V., Proshkin S.A., Kayushin A.L., Korosteleva M.L.,
RA Lebedenko E.N.;
RT "Structural and functional characterization of rpb8+ gene of
RT Schizosaccharomyces pombe encoding the subunit of RNA polymerase I-III
RT specific to eukaryotes.";
RL Bioorg. Khim. 24:118-124(1998).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=9927738; DOI=10.1093/nar/27.4.1047;
RA Voutsina A., Riva M., Carles C., Alexandraki D.;
RT "Sequence divergence of the RNA polymerase shared subunit ABC14.5 (Rpb8)
RT selectively affects RNA polymerase III assembly in Saccharomyces
RT cerevisiae.";
RL Nucleic Acids Res. 27:1047-1055(1999).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC Common component of RNA polymerases I, II and III which synthesize
CC ribosomal RNA precursors, mRNA precursors and many functional non-
CC coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively.
CC -!- SUBUNIT: Component of the RNA polymerase I (Pol I), RNA polymerase II
CC (Pol II) and RNA polymerase III (Pol III) complexes consisting of 14,
CC 12 and 17 subunits, respectively. Directly interacts with POLR2A (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the eukaryotic RPB8 RNA polymerase subunit
CC family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; D89595; BAA34367.1; -; Genomic_DNA.
DR EMBL; D86555; BAA22803.1; -; mRNA.
DR EMBL; AF020780; AAB93482.1; -; mRNA.
DR EMBL; AF029688; AAC39321.1; -; Genomic_DNA.
DR EMBL; Y07643; CAA68926.1; -; mRNA.
DR EMBL; Y07644; CAA68927.1; -; mRNA.
DR EMBL; CU329671; CAA18430.1; -; Genomic_DNA.
DR PIR; T43540; T43540.
DR PIR; T45232; T45232.
DR RefSeq; NP_595915.1; NM_001021823.2.
DR PDB; 3H0G; X-ray; 3.65 A; H/T=1-125.
DR PDB; 5U0S; EM; 7.80 A; h=1-125.
DR PDB; 7AOC; EM; 3.84 A; H=1-125.
DR PDB; 7AOD; EM; 4.50 A; H/T=1-125.
DR PDB; 7AOE; EM; 3.90 A; H=1-125.
DR PDBsum; 3H0G; -.
DR PDBsum; 5U0S; -.
DR PDBsum; 7AOC; -.
DR PDBsum; 7AOD; -.
DR PDBsum; 7AOE; -.
DR AlphaFoldDB; Q92399; -.
DR SMR; Q92399; -.
DR BioGRID; 276344; 12.
DR IntAct; Q92399; 1.
DR STRING; 4896.SPBC14C8.12.1; -.
DR MaxQB; Q92399; -.
DR PaxDb; Q92399; -.
DR EnsemblFungi; SPBC14C8.12.1; SPBC14C8.12.1:pep; SPBC14C8.12.
DR GeneID; 2539794; -.
DR KEGG; spo:SPBC14C8.12; -.
DR PomBase; SPBC14C8.12; rpb8.
DR VEuPathDB; FungiDB:SPBC14C8.12; -.
DR eggNOG; KOG3400; Eukaryota.
DR HOGENOM; CLU_103864_1_1_1; -.
DR InParanoid; Q92399; -.
DR OMA; TINAYVS; -.
DR PhylomeDB; Q92399; -.
DR Reactome; R-SPO-113418; Formation of the Early Elongation Complex.
DR Reactome; R-SPO-5578749; Transcriptional regulation by small RNAs.
DR Reactome; R-SPO-674695; RNA Polymerase II Pre-transcription Events.
DR Reactome; R-SPO-6781823; Formation of TC-NER Pre-Incision Complex.
DR Reactome; R-SPO-6782135; Dual incision in TC-NER.
DR Reactome; R-SPO-6782210; Gap-filling DNA repair synthesis and ligation in TC-NER.
DR Reactome; R-SPO-6796648; TP53 Regulates Transcription of DNA Repair Genes.
DR Reactome; R-SPO-6807505; RNA polymerase II transcribes snRNA genes.
DR Reactome; R-SPO-72086; mRNA Capping.
DR Reactome; R-SPO-72165; mRNA Splicing - Minor Pathway.
DR Reactome; R-SPO-73762; RNA Polymerase I Transcription Initiation.
DR Reactome; R-SPO-73772; RNA Polymerase I Promoter Escape.
DR Reactome; R-SPO-73776; RNA Polymerase II Promoter Escape.
DR Reactome; R-SPO-73779; RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
DR Reactome; R-SPO-75953; RNA Polymerase II Transcription Initiation.
DR Reactome; R-SPO-75955; RNA Polymerase II Transcription Elongation.
DR Reactome; R-SPO-76042; RNA Polymerase II Transcription Initiation And Promoter Clearance.
DR Reactome; R-SPO-76061; RNA Polymerase III Transcription Initiation From Type 1 Promoter.
DR Reactome; R-SPO-76066; RNA Polymerase III Transcription Initiation From Type 2 Promoter.
DR Reactome; R-SPO-77075; RNA Pol II CTD phosphorylation and interaction with CE.
DR Reactome; R-SPO-9018519; Estrogen-dependent gene expression.
DR EvolutionaryTrace; Q92399; -.
DR PRO; PR:Q92399; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0005736; C:RNA polymerase I complex; IDA:PomBase.
DR GO; GO:0005665; C:RNA polymerase II, core complex; IDA:PomBase.
DR GO; GO:0005666; C:RNA polymerase III complex; IDA:PomBase.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006360; P:transcription by RNA polymerase I; TAS:PomBase.
DR GO; GO:0006366; P:transcription by RNA polymerase II; TAS:PomBase.
DR GO; GO:0006383; P:transcription by RNA polymerase III; TAS:PomBase.
DR Gene3D; 2.40.50.140; -; 1.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR005570; RNA_pol_Rpb8.
DR PANTHER; PTHR10917; PTHR10917; 1.
DR Pfam; PF03870; RNA_pol_Rpb8; 1.
DR PIRSF; PIRSF000779; RNA_pol_Rpb8; 1.
DR SMART; SM00658; RPOL8c; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-binding; DNA-directed RNA polymerase; Nucleus;
KW Reference proteome; Transcription.
FT CHAIN 1..125
FT /note="DNA-directed RNA polymerases I, II, and III subunit
FT RPABC3"
FT /id="PRO_0000074000"
FT REGION 17..39
FT /note="Non-specific ssDNA binding"
FT /evidence="ECO:0000250"
FT CONFLICT 122..125
FT /note="LLRR -> YLEGK (in Ref. 4; CAA68926/CAA68927)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 125 AA; 14300 MW; 8941D1BB8D624770 CRC64;
MSESVLLDEI FTVTSVDKQK YQRVSRITAV SGQNDMNLTL DINSQIYPLE KDATFSLQIT
SNLNSPDLKE AADYIMYGKV YRVEEAKDEK VSVYVSFGGL LMAIEGSHRK LYRLSLDHVY
LLLRR
