RPAB4_SCHPO
ID RPAB4_SCHPO Reviewed; 63 AA.
AC P48011; O14457;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 15-JUL-1998, sequence version 2.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=DNA-directed RNA polymerases I, II, and III subunit RPABC4;
DE Short=RNA polymerases I, II, and III subunit ABC4;
DE AltName: Full=ABC10-alpha;
GN Name=rpc10; Synonyms=rpb12; ORFNames=SPBC19C2.03;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=7651387; DOI=10.1128/mcb.15.9.4702;
RA Shpakovski G.V., Acker J., Wintzerith M., Lacroix J.F., Thuriaux P.,
RA Vigneron M.;
RT "Four subunits that are shared by the three classes of RNA polymerase are
RT functionally interchangeable between Homo sapiens and Saccharomyces
RT cerevisiae.";
RL Mol. Cell. Biol. 15:4702-4710(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=9290054;
RA Shpakovskii G.V., Lebedenko E.N.;
RT "Molecular cloning and characterization of cDNA of the rpc10+ gene encoding
RT the smallest subunit of nuclear RNA polymerases of Schizosaccharomyces
RT pombe.";
RL Bioorg. Khim. 23:441-448(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=9608944;
RA Shpakovskii G.V., Proshkin S.A., Lebedenko E.N.;
RT "Exon-intron organization rpb10+ and rpc10+ genes of Schizosaccharomyces
RT pombe, coding for mini-subunits of nuclear RNA-polymerase I-III.";
RL Mol. Biol. (Mosk.) 32:285-290(1998).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=JY741;
RX PubMed=9322754; DOI=10.1016/s0378-1119(97)00222-9;
RA Sakurai H., Ishihama A.;
RT "Gene organization and protein sequence of the small subunits of
RT Schizosaccharomyces pombe RNA polymerase II.";
RL Gene 196:165-174(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC Common component of RNA polymerases I, II and III which synthesize
CC ribosomal RNA precursors, mRNA precursors and many functional non-
CC coding RNAs, and a small RNAs, such as 5S rRNA and tRNAs, respectively.
CC -!- SUBUNIT: Component of the RNA polymerase I (Pol I), RNA polymerase II
CC (Pol II) and RNA polymerase III (Pol III) complexes consisting of 14,
CC 12 and 17 subunits, respectively. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the archaeal Rpo12/eukaryotic RPC10 RNA
CC polymerase subunit family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U20867; AAA80487.1; -; mRNA.
DR EMBL; U80217; AAC49841.1; -; mRNA.
DR EMBL; AF027819; AAC16896.1; -; Genomic_DNA.
DR EMBL; D89634; BAA22807.1; -; Genomic_DNA.
DR EMBL; CU329671; CAB52030.1; -; Genomic_DNA.
DR PIR; T43546; T43546.
DR PIR; T52536; T52536.
DR RefSeq; NP_595688.1; NM_001021585.2.
DR PDB; 3H0G; X-ray; 3.65 A; L/X=1-63.
DR PDB; 5U0S; EM; 7.80 A; l=1-63.
DR PDB; 7AOC; EM; 3.84 A; L=1-63.
DR PDB; 7AOD; EM; 4.50 A; L/X=1-63.
DR PDB; 7AOE; EM; 3.90 A; L=1-63.
DR PDBsum; 3H0G; -.
DR PDBsum; 5U0S; -.
DR PDBsum; 7AOC; -.
DR PDBsum; 7AOD; -.
DR PDBsum; 7AOE; -.
DR AlphaFoldDB; P48011; -.
DR SMR; P48011; -.
DR BioGRID; 277312; 9.
DR IntAct; P48011; 1.
DR STRING; 4896.SPBC19C2.03.1; -.
DR PaxDb; P48011; -.
DR EnsemblFungi; SPBC19C2.03.1; SPBC19C2.03.1:pep; SPBC19C2.03.
DR GeneID; 2540793; -.
DR KEGG; spo:SPBC19C2.03; -.
DR PomBase; SPBC19C2.03; rpc10.
DR VEuPathDB; FungiDB:SPBC19C2.03; -.
DR eggNOG; KOG3507; Eukaryota.
DR HOGENOM; CLU_179456_0_1_1; -.
DR InParanoid; P48011; -.
DR OMA; VIRCREC; -.
DR PhylomeDB; P48011; -.
DR Reactome; R-SPO-113418; Formation of the Early Elongation Complex.
DR Reactome; R-SPO-5578749; Transcriptional regulation by small RNAs.
DR Reactome; R-SPO-674695; RNA Polymerase II Pre-transcription Events.
DR Reactome; R-SPO-6781823; Formation of TC-NER Pre-Incision Complex.
DR Reactome; R-SPO-6782135; Dual incision in TC-NER.
DR Reactome; R-SPO-6782210; Gap-filling DNA repair synthesis and ligation in TC-NER.
DR Reactome; R-SPO-6796648; TP53 Regulates Transcription of DNA Repair Genes.
DR Reactome; R-SPO-6807505; RNA polymerase II transcribes snRNA genes.
DR Reactome; R-SPO-72086; mRNA Capping.
DR Reactome; R-SPO-72165; mRNA Splicing - Minor Pathway.
DR Reactome; R-SPO-73762; RNA Polymerase I Transcription Initiation.
DR Reactome; R-SPO-73772; RNA Polymerase I Promoter Escape.
DR Reactome; R-SPO-73776; RNA Polymerase II Promoter Escape.
DR Reactome; R-SPO-73779; RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
DR Reactome; R-SPO-75953; RNA Polymerase II Transcription Initiation.
DR Reactome; R-SPO-75955; RNA Polymerase II Transcription Elongation.
DR Reactome; R-SPO-76042; RNA Polymerase II Transcription Initiation And Promoter Clearance.
DR Reactome; R-SPO-76061; RNA Polymerase III Transcription Initiation From Type 1 Promoter.
DR Reactome; R-SPO-76066; RNA Polymerase III Transcription Initiation From Type 2 Promoter.
DR Reactome; R-SPO-77075; RNA Pol II CTD phosphorylation and interaction with CE.
DR Reactome; R-SPO-9018519; Estrogen-dependent gene expression.
DR EvolutionaryTrace; P48011; -.
DR PRO; PR:P48011; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0005730; C:nucleolus; HDA:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0005736; C:RNA polymerase I complex; IGI:PomBase.
DR GO; GO:0005665; C:RNA polymerase II, core complex; IDA:PomBase.
DR GO; GO:0005666; C:RNA polymerase III complex; IGI:PomBase.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006360; P:transcription by RNA polymerase I; IGI:PomBase.
DR GO; GO:0006366; P:transcription by RNA polymerase II; IGI:PomBase.
DR GO; GO:0006383; P:transcription by RNA polymerase III; IGI:PomBase.
DR InterPro; IPR006591; RNAP_P/RPABC4.
DR InterPro; IPR039747; RPABC4.
DR InterPro; IPR029040; RPABC4/Spt4.
DR PANTHER; PTHR12056; PTHR12056; 1.
DR Pfam; PF03604; DNA_RNApol_7kD; 1.
DR SMART; SM00659; RPOLCX; 1.
DR SUPFAM; SSF63393; SSF63393; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-directed RNA polymerase; Metal-binding; Nucleus;
KW Reference proteome; Transcription; Zinc; Zinc-finger.
FT CHAIN 1..63
FT /note="DNA-directed RNA polymerases I, II, and III subunit
FT RPABC4"
FT /id="PRO_0000159752"
FT ZN_FING 24..44
FT /note="C4-type"
FT /evidence="ECO:0000255"
FT BINDING 24
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
FT BINDING 27
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
FT BINDING 41
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
FT BINDING 44
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
FT CONFLICT 29
FT /note="A -> R (in Ref. 1 and 2)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 63 AA; 7203 MW; 97EBE50FAA95E884 CRC64;
MNHPTSTGGT AFNPPRPATM IYLCADCGAR NTIQAKEVIR CRECGHRVMY KMRTKRMVQF
EAR
