RPAB5_SCHPO
ID RPAB5_SCHPO Reviewed; 71 AA.
AC O13877; O14458;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 2.
DT 03-AUG-2022, entry version 160.
DE RecName: Full=DNA-directed RNA polymerases I, II, and III subunit RPABC5;
DE Short=RNA polymerases I, II, and III subunit ABC5;
DE AltName: Full=ABC10-beta;
DE AltName: Full=DNA-directed RNA polymerases I, II, and III 8.3 kDa polypeptide;
DE AltName: Full=RPC8;
GN Name=rpb10; ORFNames=SPAC1B3.12c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=9054344;
RA Shpakovskii G.V., Lebedenko E.N.;
RT "Three regions of Rpb10 mini-subunit of nuclear RNA polymerases are
RT strictly conserved in all eukaryotes.";
RL Bioorg. Khim. 22:938-940(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=JY741;
RX PubMed=9322754; DOI=10.1016/s0378-1119(97)00222-9;
RA Sakurai H., Ishihama A.;
RT "Gene organization and protein sequence of the small subunits of
RT Schizosaccharomyces pombe RNA polymerase II.";
RL Gene 196:165-174(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=9608944;
RA Shpakovskii G.V., Proshkin S.A., Lebedenko E.N.;
RT "Exon-intron organization rpb10+ and rpc10+ genes of Schizosaccharomyces
RT pombe, coding for mini-subunits of nuclear RNA-polymerase I-III.";
RL Mol. Biol. (Mosk.) 32:285-290(1998).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [5]
RP X-RAY CRYSTALLOGRAPHY (3.6 ANGSTROMS) OF THE RNA POL II CORE COMPLEX IN
RP COMPLEX WITH ZINC.
RX PubMed=19458260; DOI=10.1073/pnas.0903361106;
RA Spaahr H., Calero G., Bushnell D.A., Kornberg R.D.;
RT "Schizosacharomyces pombe RNA polymerase II at 3.6-A resolution.";
RL Proc. Natl. Acad. Sci. U.S.A. 106:9185-9190(2009).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC Common component of RNA polymerases I, II and III which synthesize
CC ribosomal RNA precursors, mRNA precursors and many functional non-
CC coding RNAs, and a small RNAs, such as 5S rRNA and tRNAs, respectively.
CC Pol II is the central component of the basal RNA polymerase II
CC transcription machinery. Pols are composed of mobile elements that move
CC relative to each other. In Pol II, RBP10 is part of the core element
CC with the central large cleft (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the RNA polymerase I (Pol I), RNA polymerase II
CC (Pol II) and RNA polymerase III (Pol III) complexes consisting of 14,
CC 12 and 17 subunits, respectively. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the archaeal Rpo10/eukaryotic RPB10 RNA
CC polymerase subunit family. {ECO:0000305}.
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DR EMBL; X95733; CAA65049.1; -; mRNA.
DR EMBL; U80219; AAC49842.1; -; mRNA.
DR EMBL; D89596; BAA22805.1; -; Genomic_DNA.
DR EMBL; AF027818; AAC16895.1; -; Genomic_DNA.
DR EMBL; CU329670; CAB11246.1; -; Genomic_DNA.
DR PIR; T43545; T43545.
DR RefSeq; NP_594797.1; NM_001020225.2.
DR PDB; 3H0G; X-ray; 3.65 A; J/V=1-71.
DR PDB; 5U0S; EM; 7.80 A; j=1-71.
DR PDB; 7AOC; EM; 3.84 A; J=1-71.
DR PDB; 7AOD; EM; 4.50 A; J/V=1-71.
DR PDB; 7AOE; EM; 3.90 A; J=1-71.
DR PDBsum; 3H0G; -.
DR PDBsum; 5U0S; -.
DR PDBsum; 7AOC; -.
DR PDBsum; 7AOD; -.
DR PDBsum; 7AOE; -.
DR AlphaFoldDB; O13877; -.
DR SMR; O13877; -.
DR BioGRID; 278705; 13.
DR IntAct; O13877; 1.
DR STRING; 4896.SPAC1B3.12c.1; -.
DR MaxQB; O13877; -.
DR PaxDb; O13877; -.
DR EnsemblFungi; SPAC1B3.12c.1; SPAC1B3.12c.1:pep; SPAC1B3.12c.
DR GeneID; 2542233; -.
DR KEGG; spo:SPAC1B3.12c; -.
DR PomBase; SPAC1B3.12c; rpb10.
DR VEuPathDB; FungiDB:SPAC1B3.12c; -.
DR eggNOG; KOG3497; Eukaryota.
DR HOGENOM; CLU_143122_2_1_1; -.
DR InParanoid; O13877; -.
DR OMA; YCCRRMF; -.
DR PhylomeDB; O13877; -.
DR Reactome; R-SPO-113418; Formation of the Early Elongation Complex.
DR Reactome; R-SPO-5578749; Transcriptional regulation by small RNAs.
DR Reactome; R-SPO-674695; RNA Polymerase II Pre-transcription Events.
DR Reactome; R-SPO-6781823; Formation of TC-NER Pre-Incision Complex.
DR Reactome; R-SPO-6782135; Dual incision in TC-NER.
DR Reactome; R-SPO-6782210; Gap-filling DNA repair synthesis and ligation in TC-NER.
DR Reactome; R-SPO-6796648; TP53 Regulates Transcription of DNA Repair Genes.
DR Reactome; R-SPO-6807505; RNA polymerase II transcribes snRNA genes.
DR Reactome; R-SPO-72086; mRNA Capping.
DR Reactome; R-SPO-72165; mRNA Splicing - Minor Pathway.
DR Reactome; R-SPO-73762; RNA Polymerase I Transcription Initiation.
DR Reactome; R-SPO-73772; RNA Polymerase I Promoter Escape.
DR Reactome; R-SPO-73776; RNA Polymerase II Promoter Escape.
DR Reactome; R-SPO-73779; RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
DR Reactome; R-SPO-75953; RNA Polymerase II Transcription Initiation.
DR Reactome; R-SPO-75955; RNA Polymerase II Transcription Elongation.
DR Reactome; R-SPO-76042; RNA Polymerase II Transcription Initiation And Promoter Clearance.
DR Reactome; R-SPO-76061; RNA Polymerase III Transcription Initiation From Type 1 Promoter.
DR Reactome; R-SPO-76066; RNA Polymerase III Transcription Initiation From Type 2 Promoter.
DR Reactome; R-SPO-77075; RNA Pol II CTD phosphorylation and interaction with CE.
DR Reactome; R-SPO-9018519; Estrogen-dependent gene expression.
DR EvolutionaryTrace; O13877; -.
DR PRO; PR:O13877; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0005730; C:nucleolus; HDA:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0005736; C:RNA polymerase I complex; IGI:PomBase.
DR GO; GO:0005665; C:RNA polymerase II, core complex; IDA:PomBase.
DR GO; GO:0005666; C:RNA polymerase III complex; IGI:PomBase.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IBA:GO_Central.
DR GO; GO:0006360; P:transcription by RNA polymerase I; IGI:PomBase.
DR GO; GO:0006366; P:transcription by RNA polymerase II; IGI:PomBase.
DR GO; GO:0006383; P:transcription by RNA polymerase III; IGI:PomBase.
DR GO; GO:0042797; P:tRNA transcription by RNA polymerase III; IBA:GO_Central.
DR HAMAP; MF_00250; RNApol_arch_Rpo10; 1.
DR InterPro; IPR023580; RNA_pol_su_RPB10.
DR InterPro; IPR020789; RNA_pol_suN_Zn-BS.
DR InterPro; IPR000268; RPABC5/Rpb10.
DR PANTHER; PTHR23431; PTHR23431; 1.
DR Pfam; PF01194; RNA_pol_N; 1.
DR PIRSF; PIRSF005653; RNA_pol_N/8_sub; 1.
DR SUPFAM; SSF46924; SSF46924; 1.
DR PROSITE; PS01112; RNA_POL_N_8KD; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-directed RNA polymerase; Metal-binding; Nucleus;
KW Reference proteome; Transcription; Zinc.
FT CHAIN 1..71
FT /note="DNA-directed RNA polymerases I, II, and III subunit
FT RPABC5"
FT /id="PRO_0000121341"
FT BINDING 7
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000269|PubMed:19458260,
FT ECO:0007744|PDB:3H0G"
FT BINDING 10
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000269|PubMed:19458260,
FT ECO:0007744|PDB:3H0G"
FT BINDING 44
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000269|PubMed:19458260,
FT ECO:0007744|PDB:3H0G"
FT BINDING 45
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000269|PubMed:19458260,
FT ECO:0007744|PDB:3H0G"
SQ SEQUENCE 71 AA; 8276 MW; 8B9EEE8FD59A2458 CRC64;
MIIPIRCFSC GKVIGDKWDT YLTLLQEDNT EGEALDKLGL QRYCCRRMIL THVDLIEKLL
CYNPLSKQKN L
