RPA_METJA
ID RPA_METJA Reviewed; 645 AA.
AC Q58559;
DT 26-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 25-MAY-2022, entry version 111.
DE RecName: Full=Replication factor A;
DE Short=RF-A;
DE Short=RP-A;
DE AltName: Full=Replication factor A protein 1;
DE AltName: Full=Replication protein A;
DE AltName: Full=Single-stranded DNA-binding protein;
DE AltName: Full=mjaSSB;
GN Name=rpa; OrderedLocusNames=MJ1159;
OS Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS 10045 / NBRC 100440) (Methanococcus jannaschii).
OC Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC Methanocaldococcaceae; Methanocaldococcus.
OX NCBI_TaxID=243232;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT jannaschii.";
RL Science 273:1058-1073(1996).
RN [2]
RP CHARACTERIZATION, SUBUNIT, AND DNA-BINDING.
RC STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX PubMed=9843941; DOI=10.1073/pnas.95.25.14634;
RA Kelly T.J., Simancek P., Brush G.S.;
RT "Identification and characterization of a single-stranded DNA-binding
RT protein from the archaeon Methanococcus jannaschii.";
RL Proc. Natl. Acad. Sci. U.S.A. 95:14634-14639(1998).
RN [3]
RP X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 170-274.
RG Northeast structural genomics consortium (NESG);
RT "Crystal structure of a domain of a replication factor a protein, from
RT Methanocaldococcus jannaschii. Northeast structural genomics target
RT mjr118e.";
RL Submitted (FEB-2009) to the PDB data bank.
CC -!- FUNCTION: Probably plays an essential for replication of the
CC chromosome, DNA recombination and repair (By similarity). Binds
CC approximately 20 nucleotides. {ECO:0000250}.
CC -!- SUBUNIT: Monomer. Binds to single-stranded DNA sequences.
CC {ECO:0000269|PubMed:9843941}.
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DR EMBL; L77117; AAB99162.1; -; Genomic_DNA.
DR PIR; F64444; F64444.
DR RefSeq; WP_010870672.1; NC_000909.1.
DR PDB; 3DM3; X-ray; 2.40 A; A/B/C=170-274.
DR PDBsum; 3DM3; -.
DR AlphaFoldDB; Q58559; -.
DR SMR; Q58559; -.
DR STRING; 243232.MJ_1159; -.
DR EnsemblBacteria; AAB99162; AAB99162; MJ_1159.
DR GeneID; 1452057; -.
DR KEGG; mja:MJ_1159; -.
DR eggNOG; arCOG01510; Archaea.
DR HOGENOM; CLU_415991_0_0_2; -.
DR InParanoid; Q58559; -.
DR OMA; NAFSRKW; -.
DR OrthoDB; 8528at2157; -.
DR PhylomeDB; Q58559; -.
DR EvolutionaryTrace; Q58559; -.
DR Proteomes; UP000000805; Chromosome.
DR GO; GO:0003677; F:DNA binding; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; IBA:GO_Central.
DR GO; GO:0010212; P:response to ionizing radiation; IBA:GO_Central.
DR Gene3D; 2.40.50.140; -; 5.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR004365; NA-bd_OB_tRNA.
DR InterPro; IPR013955; Rep_factor-A_C.
DR Pfam; PF08646; Rep_fac-A_C; 1.
DR Pfam; PF01336; tRNA_anti-codon; 3.
DR SUPFAM; SSF50249; SSF50249; 5.
PE 1: Evidence at protein level;
KW 3D-structure; DNA damage; DNA repair; DNA replication; DNA-binding;
KW Metal-binding; Reference proteome; Repeat; Zinc; Zinc-finger.
FT CHAIN 1..645
FT /note="Replication factor A"
FT /id="PRO_0000097430"
FT DNA_BIND 78..158
FT /note="OB 1"
FT DNA_BIND 186..266
FT /note="OB 2"
FT DNA_BIND 291..378
FT /note="OB 3"
FT DNA_BIND 423..497
FT /note="OB 4"
FT ZN_FING 531..550
FT /note="C4-type"
FT /evidence="ECO:0000255"
FT HELIX 177..179
FT /evidence="ECO:0007829|PDB:3DM3"
FT STRAND 182..202
FT /evidence="ECO:0007829|PDB:3DM3"
FT STRAND 208..218
FT /evidence="ECO:0007829|PDB:3DM3"
FT STRAND 221..228
FT /evidence="ECO:0007829|PDB:3DM3"
FT HELIX 229..233
FT /evidence="ECO:0007829|PDB:3DM3"
FT STRAND 241..250
FT /evidence="ECO:0007829|PDB:3DM3"
FT STRAND 254..267
FT /evidence="ECO:0007829|PDB:3DM3"
SQ SEQUENCE 645 AA; 73842 MW; 3F0AD1B78065DD4F CRC64;
MIGDYERFKQ LKKKVAEALN ISEEELDRMI DKKIEENGGI ILKDAALMMI AKEHGVYGEE
KNDEEFLISD IEEGQIGVEI TGVITDISEI KTFKRRDGSL GKYKRITIAD KSGTIRMTLW
DDLAELDVKV GDVIKIERAR ARKWRNNLEL SSTSETKIKK LENYEGELPE IKDTYNIGEL
SPGMTATFEG EVISALPIKE FKRADGSIGK LKSFIVRDET GSIRVTLWDN LTDIDVGRGD
YVRVRGYIRE GYYGGLECTA NYVEILKKGE KIESEEVNIE DLTKYEDGEL VSVKGRVIAI
SNKKSVDLDG EIAKVQDIIL DNGTGRVRVS FWRGKTALLE NIKEGDLVRI TNCRVKTFYD
REGNKRTDLV ATLETEVIKD ENIEAPEYEL KYCKIEDIYN RDVDWNDINL IAQVVEDYGV
NEIEFEDKVR KVRNLLLEDG TGRIRLSLWD DLAEIEIKEG DIVEILHAYA KERGDYIDLV
IGKYGRIIIN PEGVEIKTNR KFIADIEDGE TVEVRGAVVK ILSDTLFLYL CPNCRKRVVE
IDGIYNCPIC GDVEPEEILR LNFVVDDGTG TLLCRAYDRR VEKMLKMNRE ELKNLTIEMV
EDEILGEEFV LYGNVRVEND ELIMVVRRVN DVDVEKEIRI LEEME
