RPB1B_HUMAN
ID RPB1B_HUMAN Reviewed; 115 AA.
AC Q9GZM3; F6W009; Q9H1A8;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 2.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=DNA-directed RNA polymerase II subunit RPB11-b1;
DE Short=RNA polymerase II subunit B11-b1;
DE Short=RPB11b1;
DE AltName: Full=DNA-directed RNA polymerase II subunit J2;
GN Name=POLR2J2;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2), TISSUE
RP SPECIFICITY, AND VARIANT ASN-28.
RC TISSUE=Cervix carcinoma, and Placenta;
RX PubMed=11747469; DOI=10.1186/1471-2199-2-14;
RA Grandemange S., Schaller S., Yamano S., Du Manoir S., Shpakovski G.V.,
RA Mattei M.-G., Kedinger C., Vigneron M.;
RT "A human RNA polymerase II subunit is encoded by a recently generated
RT multigene family.";
RL BMC Mol. Biol. 2:14-14(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12853948; DOI=10.1038/nature01782;
RA Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H.,
RA Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K.,
RA Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A.,
RA Delehaunty K.D., Miner T.L., Nash W.E., Cordes M., Du H., Sun H.,
RA Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A.,
RA Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P.,
RA Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M.,
RA Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S.,
RA Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R.,
RA Strowmatt C., Latreille P., Miller N., Johnson D., Murray J.,
RA Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W.,
RA Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A.,
RA Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E.,
RA Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E.,
RA Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A.,
RA Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A.,
RA Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R.,
RA McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H.,
RA Wilson R.K.;
RT "The DNA sequence of human chromosome 7.";
RL Nature 424:157-164(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND VARIANT ASN-28.
RC TISSUE=Lung;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP INTERACTION WITH SATB1.
RX PubMed=12036295; DOI=10.1006/geno.2002.6772;
RA Durrin L.K., Krontiris T.G.;
RT "The thymocyte-specific MAR binding protein, SATB1, interacts in vitro with
RT a novel variant of DNA-directed RNA polymerase II, subunit 11.";
RL Genomics 79:809-817(2002).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC Component of RNA polymerase II which synthesizes mRNA precursors and
CC many functional non-coding RNAs. Pol II is the central component of the
CC basal RNA polymerase II transcription machinery. It is composed of
CC mobile elements that move relative to each other. RPB11 is part of the
CC core element with the central large cleft (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Component of the RNA polymerase II (Pol II) complex consisting
CC of 12 subunits.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=RPB11b1-alpha;
CC IsoId=Q9GZM3-1; Sequence=Displayed;
CC Name=2; Synonyms=RPB11b1-beta;
CC IsoId=Q9GZM3-2; Sequence=VSP_030820;
CC -!- TISSUE SPECIFICITY: Ubiquitously expressed.
CC {ECO:0000269|PubMed:11747469}.
CC -!- SIMILARITY: Belongs to the archaeal Rpo11/eukaryotic RPB11/RPC19 RNA
CC polymerase subunit family. {ECO:0000305}.
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DR EMBL; AJ277931; CAC18332.1; -; Genomic_DNA.
DR EMBL; AJ277739; CAC18329.1; -; mRNA.
DR EMBL; AJ277740; CAC18330.1; -; mRNA.
DR EMBL; AC105052; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC086857; AAH86857.1; -; mRNA.
DR CCDS; CCDS43627.1; -. [Q9GZM3-1]
DR RefSeq; NP_001091084.2; NM_001097615.2.
DR RefSeq; NP_116581.3; NM_032959.5. [Q9GZM3-1]
DR RefSeq; XP_016867861.1; XM_017012372.1.
DR RefSeq; XP_016867862.1; XM_017012373.1.
DR AlphaFoldDB; Q9GZM3; -.
DR SMR; Q9GZM3; -.
DR BioGRID; 128910; 3.
DR BioGRID; 139239; 34.
DR STRING; 9606.ENSP00000330898; -.
DR iPTMnet; Q9GZM3; -.
DR PhosphoSitePlus; Q9GZM3; -.
DR BioMuta; POLR2J2; -.
DR jPOST; Q9GZM3; -.
DR MassIVE; Q9GZM3; -.
DR MaxQB; Q9GZM3; -.
DR PaxDb; Q9GZM3; -.
DR PeptideAtlas; Q9GZM3; -.
DR PRIDE; Q9GZM3; -.
DR ProteomicsDB; 28095; -.
DR ProteomicsDB; 80086; -. [Q9GZM3-2]
DR Antibodypedia; 57740; 88 antibodies from 12 providers.
DR DNASU; 246721; -.
DR Ensembl; ENST00000333432.8; ENSP00000330898.7; ENSG00000228049.7. [Q9GZM3-1]
DR GeneID; 246721; -.
DR GeneID; 548644; -.
DR KEGG; hsa:246721; -.
DR KEGG; hsa:548644; -.
DR MANE-Select; ENST00000333432.9; ENSP00000330898.7; NM_032959.7; NP_116581.3.
DR UCSC; uc003vah.4; human. [Q9GZM3-1]
DR CTD; 246721; -.
DR CTD; 548644; -.
DR GeneCards; POLR2J2; -.
DR HGNC; HGNC:23208; POLR2J2.
DR HPA; ENSG00000228049; Low tissue specificity.
DR MIM; 609881; gene.
DR neXtProt; NX_Q9GZM3; -.
DR PharmGKB; PA164724655; -.
DR VEuPathDB; HostDB:ENSG00000228049; -.
DR eggNOG; KOG4392; Eukaryota.
DR GeneTree; ENSGT00550000074975; -.
DR GeneTree; ENSGT00940000164393; -.
DR HOGENOM; CLU_090381_2_2_1; -.
DR InParanoid; Q9GZM3; -.
DR OMA; LEQACTK; -.
DR TreeFam; TF103044; -.
DR PathwayCommons; Q9GZM3; -.
DR SIGNOR; Q9GZM3; -.
DR BioGRID-ORCS; 246721; 47 hits in 292 CRISPR screens.
DR BioGRID-ORCS; 548644; 76 hits in 278 CRISPR screens.
DR ChiTaRS; POLR2J2; human.
DR GeneWiki; POLR2J2; -.
DR Pharos; Q9GZM3; Tdark.
DR PRO; PR:Q9GZM3; -.
DR Proteomes; UP000005640; Chromosome 7.
DR RNAct; Q9GZM3; protein.
DR Bgee; ENSG00000228049; Expressed in blood and 96 other tissues.
DR ExpressionAtlas; Q9GZM3; baseline and differential.
DR Genevisible; Q9GZM3; HS.
DR GO; GO:0005665; C:RNA polymerase II, core complex; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0001055; F:RNA polymerase II activity; IEA:InterPro.
DR CDD; cd06926; RNAP_II_RPB11; 1.
DR Gene3D; 3.30.1360.10; -; 1.
DR HAMAP; MF_00261; RNApol_arch_Rpo11; 1.
DR InterPro; IPR037685; RBP11.
DR InterPro; IPR036603; RBP11-like.
DR InterPro; IPR009025; RBP11-like_dimer.
DR InterPro; IPR008193; RNA_pol_Rpb11_13-16kDa_CS.
DR Pfam; PF13656; RNA_pol_L_2; 1.
DR SUPFAM; SSF55257; SSF55257; 1.
DR PROSITE; PS01154; RNA_POL_L_13KD; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-directed RNA polymerase; Nucleus;
KW Reference proteome; Transcription.
FT CHAIN 1..115
FT /note="DNA-directed RNA polymerase II subunit RPB11-b1"
FT /id="PRO_0000316946"
FT VAR_SEQ 48..115
FT /note="QLLKDPQVLFAGYKVPHPLEHKIIIRVQTTPDYSPQEAFTNAITDLISELSL
FT LEERFRTCLLPLRLLP -> RACFPFAFCRDCQFPEASPATLPVQPAELCPRAHQLCAP
FT ALKRHLGGEAHPVHLHAGAASRPVQQPQHL (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:11747469,
FT ECO:0000303|PubMed:15489334"
FT /id="VSP_030820"
FT VARIANT 28
FT /note="K -> N (in dbSNP:rs62483491)"
FT /evidence="ECO:0000269|PubMed:11747469,
FT ECO:0000269|PubMed:15489334"
FT /id="VAR_070807"
SQ SEQUENCE 115 AA; 13088 MW; 05310B30C071AD1E CRC64;
MNAPPAFESF LLFEGEKITI NKDTKVPKAC LFTINKEDHT LGNIIKSQLL KDPQVLFAGY
KVPHPLEHKI IIRVQTTPDY SPQEAFTNAI TDLISELSLL EERFRTCLLP LRLLP
