ID   RPB3_ASFB7              Reviewed;         359 AA.
AC   Q65184;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 91.
DE   RecName: Full=DNA-directed RNA polymerase RPB3-11 homolog {ECO:0000303|PubMed:23041356, ECO:0000303|PubMed:32725217};
DE            Short=RPB3-11 homolog {ECO:0000305};
GN   OrderedLocusNames=Ba71V-113; ORFNames=H359L;
OS   African swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V)
OS   (ASFV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Asfuvirales; Asfarviridae; Asfivirus.
OX   NCBI_TaxID=10498;
OH   NCBI_TaxID=6937; Ornithodoros (relapsing fever ticks).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=11831707; DOI=10.1006/viro.1995.1149;
RA   Yanez R.J., Rodriguez J.M., Nogal M.L., Yuste L., Enriquez C.,
RA   Rodriguez J.F., Vinuela E.;
RT   "Analysis of the complete nucleotide sequence of African swine fever
RT   virus.";
RL   Virology 208:249-278(1995).
RN   [2]
RP   REVIEW.
RX   PubMed=23041356; DOI=10.1016/j.virusres.2012.09.014;
RA   Rodriguez J.M., Salas M.L.;
RT   "African swine fever virus transcription.";
RL   Virus Res. 173:15-28(2013).
RN   [3]
RP   SUBCELLULAR LOCATION.
RX   PubMed=30185597; DOI=10.1128/jvi.01293-18;
RA   Alejo A., Matamoros T., Guerra M., Andres G.;
RT   "A Proteomic Atlas of the African Swine Fever Virus Particle.";
RL   J. Virol. 92:0-0(2018).
RN   [4]
RP   REVIEW.
RX   PubMed=32725217; DOI=10.1042/bst20191108;
RA   Cackett G., Sykora M., Werner F.;
RT   "Transcriptome view of a killer: African swine fever virus.";
RL   Biochem. Soc. Trans. 48:1569-1581(2020).
RN   [5]
RP   INDUCTION.
RX   PubMed=32075923; DOI=10.1128/jvi.00119-20;
RA   Cackett G., Matelska D., Sykora M., Portugal R., Malecki M., Baehler J.,
RA   Dixon L., Werner F.;
RT   "The African Swine Fever Virus Transcriptome.";
RL   J. Virol. 94:0-0(2020).
CC   -!- FUNCTION: Component of the DNA-directed RNA polymerase (RNAP) that
CC       catalyzes the transcription in the cytoplasm of viral DNA into RNA
CC       using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000250|UniProtKB:P19387}.
CC   -!- SUBUNIT: Part of the viral DNA-directed RNA polymerase that consists of
CC       8 polII-like subunits (RPB1, RPB2, RPB3, RPB5, RPB6, RPB7, RPB9,
CC       RPB10), a capping enzyme and a termination factor.
CC       {ECO:0000303|PubMed:32725217}.
CC   -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000305}. Virion
CC       {ECO:0000269|PubMed:30185597}. Note=Found in association with viral
CC       nucleoid. {ECO:0000269|PubMed:30185597}.
CC   -!- INDUCTION: Expressed in the early phase of the viral replicative cycle.
CC       {ECO:0000269|PubMed:32075923}.
CC   -!- SIMILARITY: In the N-terminal section; belongs to the archaeal
CC       RpoD/eukaryotic RPB3 RNA polymerase subunit family. {ECO:0000305}.
CC   -!- SIMILARITY: In the C-terminal section; belongs to the archaeal
CC       RpoL/eukaryotic RPB11/RPC19 RNA polymerase subunit family.
CC       {ECO:0000305}.
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DR   EMBL; U18466; AAA65342.1; -; Genomic_DNA.
DR   RefSeq; NP_042806.1; NC_001659.2.
DR   SMR; Q65184; -.
DR   GeneID; 22220342; -.
DR   KEGG; vg:22220342; -.
DR   Proteomes; UP000000624; Genome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   GO; GO:0019083; P:viral transcription; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.1360.10; -; 1.
DR   InterPro; IPR036603; RBP11-like.
DR   InterPro; IPR009025; RBP11-like_dimer.
DR   InterPro; IPR036643; RNApol_insert_sf.
DR   Pfam; PF13656; RNA_pol_L_2; 1.
DR   SUPFAM; SSF55257; SSF55257; 1.
DR   SUPFAM; SSF56553; SSF56553; 1.
PE   2: Evidence at transcript level;
KW   DNA-directed RNA polymerase; Early protein; Host cytoplasm;
KW   Reference proteome; Transcription; Viral transcription; Virion.
FT   CHAIN           1..359
FT                   /note="DNA-directed RNA polymerase RPB3-11 homolog"
FT                   /id="PRO_0000373164"
SQ   SEQUENCE   359 AA;  41300 MW;  927E42E3E7DB01A1 CRC64;
     MEKIFQNVEI KPFLIDFSNP FIKNAAKRLF QLEEQLPLVP VNVVMDFKGI SRAAVHGLSR
     VLQDEIPNYM LDIKPGGYKI EDSTDLFMTE QFIRNRINFI PIYAKNETLV FALRSLNNSC
     EVKTIYSRDL IQVAGPKLKY PIFNPTFEIG FLQPGKSLII EDIYIKKGIG RKHAAFNLAV
     KTHFSHLDIE QYPTDKKEYM ALSGYKQSSM TSDPRHHRLG LCFPAVPLPH INQAVRTYLK
     NACRIIIGRI QSIQKIYENF EEPQPELVLF SLDEEKTKAI ITIKDETHTI GNLLKTCIYE
     MIPDISFVGY QCVPHKQEMV LTIIHKASQE DLITLLEKSI QNIIQTFQIL EKNVDELIA