ID   RPB4_SCHPO              Reviewed;         135 AA.
AC   O74825;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   03-AUG-2022, entry version 153.
DE   RecName: Full=DNA-directed RNA polymerase II subunit rpb4;
DE            Short=RNA polymerase II subunit B4;
GN   Name=rpb4; ORFNames=SPBC337.14;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=JY741;
RX   PubMed=10523639; DOI=10.1128/mcb.19.11.7511;
RA   Sakurai H., Mitsuzawa H., Kimura M., Ishihama A.;
RT   "The Rpb4 subunit of fission yeast Schizosaccharomyces pombe RNA polymerase
RT   II is essential for cell viability and similar in structure to the
RT   corresponding subunits of higher eukaryotes.";
RL   Mol. Cell. Biol. 19:7511-7518(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=10734555;
RA   Shpakovski G.V., Baranova G.M.;
RT   "RNA polymerase II from Schizosaccharomyces pombe contains 12 different
RT   subunits: identification and characterization of subunit Rpb4.";
RL   Bioorg. Khim. 25:938-942(1999).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       Component of RNA polymerase II which synthesizes mRNA precursors and
CC       many functional non-coding RNAs. Pol II is the central component of the
CC       basal RNA polymerase II transcription machinery. It is composed of
CC       mobile elements that move relative to each other. RPB4 is part of a
CC       subcomplex with RPB7 that binds to a pocket formed by RPB1, RPB2 and
CC       RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems
CC       to lock the clamp via RPB7 in the closed conformation thus preventing
CC       double-stranded DNA to enter the active site cleft. The RPB4-RPB7
CC       subcomplex binds single-stranded DNA and RNA (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Component of the RNA polymerase II (Pol II) complex consisting
CC       of 12 subunits. RPB4 and RPB7 form a Pol II subcomplex (By similarity).
CC       {ECO:0000250}.
CC   -!- INTERACTION:
CC       O74825; O94646: med8; NbExp=3; IntAct=EBI-697320, EBI-1533413;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the eukaryotic RPB4 RNA polymerase subunit
CC       family. {ECO:0000305}.
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DR   EMBL; AB019575; BAA85621.1; -; Genomic_DNA.
DR   EMBL; AF149308; AAF62856.1; -; mRNA.
DR   EMBL; CU329671; CAA21284.1; -; Genomic_DNA.
DR   PIR; T40267; T40267.
DR   RefSeq; NP_595415.1; NM_001021322.2.
DR   PDB; 3H0G; X-ray; 3.65 A; D/P=1-135.
DR   PDB; 5U0S; EM; 7.80 A; d=1-135.
DR   PDBsum; 3H0G; -.
DR   PDBsum; 5U0S; -.
DR   AlphaFoldDB; O74825; -.
DR   SMR; O74825; -.
DR   BioGRID; 277526; 13.
DR   IntAct; O74825; 3.
DR   MINT; O74825; -.
DR   STRING; 4896.SPBC337.14.1; -.
DR   MaxQB; O74825; -.
DR   PaxDb; O74825; -.
DR   EnsemblFungi; SPBC337.14.1; SPBC337.14.1:pep; SPBC337.14.
DR   GeneID; 2541011; -.
DR   KEGG; spo:SPBC337.14; -.
DR   PomBase; SPBC337.14; rpb4.
DR   VEuPathDB; FungiDB:SPBC337.14; -.
DR   eggNOG; KOG2351; Eukaryota.
DR   HOGENOM; CLU_110332_2_1_1; -.
DR   InParanoid; O74825; -.
DR   OMA; AQLGTLC; -.
DR   PhylomeDB; O74825; -.
DR   Reactome; R-SPO-113418; Formation of the Early Elongation Complex.
DR   Reactome; R-SPO-5578749; Transcriptional regulation by small RNAs.
DR   Reactome; R-SPO-674695; RNA Polymerase II Pre-transcription Events.
DR   Reactome; R-SPO-6781823; Formation of TC-NER Pre-Incision Complex.
DR   Reactome; R-SPO-6782135; Dual incision in TC-NER.
DR   Reactome; R-SPO-6782210; Gap-filling DNA repair synthesis and ligation in TC-NER.
DR   Reactome; R-SPO-6796648; TP53 Regulates Transcription of DNA Repair Genes.
DR   Reactome; R-SPO-6807505; RNA polymerase II transcribes snRNA genes.
DR   Reactome; R-SPO-72086; mRNA Capping.
DR   Reactome; R-SPO-72165; mRNA Splicing - Minor Pathway.
DR   Reactome; R-SPO-73776; RNA Polymerase II Promoter Escape.
DR   Reactome; R-SPO-73779; RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
DR   Reactome; R-SPO-75953; RNA Polymerase II Transcription Initiation.
DR   Reactome; R-SPO-75955; RNA Polymerase II Transcription Elongation.
DR   Reactome; R-SPO-76042; RNA Polymerase II Transcription Initiation And Promoter Clearance.
DR   Reactome; R-SPO-77075; RNA Pol II CTD phosphorylation and interaction with CE.
DR   Reactome; R-SPO-9018519; Estrogen-dependent gene expression.
DR   EvolutionaryTrace; O74825; -.
DR   PRO; PR:O74825; -.
DR   Proteomes; UP000002485; Chromosome II.
DR   GO; GO:0005634; C:nucleus; HDA:PomBase.
DR   GO; GO:0000932; C:P-body; IBA:GO_Central.
DR   GO; GO:0005665; C:RNA polymerase II, core complex; IDA:PomBase.
DR   GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR   GO; GO:0031369; F:translation initiation factor binding; IBA:GO_Central.
DR   GO; GO:0031990; P:mRNA export from nucleus in response to heat stress; IBA:GO_Central.
DR   GO; GO:0000288; P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay; IBA:GO_Central.
DR   GO; GO:0045948; P:positive regulation of translational initiation; IBA:GO_Central.
DR   GO; GO:0034402; P:recruitment of 3'-end processing factors to RNA polymerase II holoenzyme complex; IBA:GO_Central.
DR   GO; GO:0006366; P:transcription by RNA polymerase II; IMP:PomBase.
DR   GO; GO:0006367; P:transcription initiation from RNA polymerase II promoter; IBA:GO_Central.
DR   Gene3D; 1.20.1250.40; -; 1.
DR   InterPro; IPR010997; HRDC-like_sf.
DR   InterPro; IPR006590; RNA_pol_Rpb4/RPC9_core.
DR   InterPro; IPR045222; Rpb4-like.
DR   InterPro; IPR005574; Rpb4/RPC9.
DR   InterPro; IPR038324; Rpb4/RPC9_sf.
DR   PANTHER; PTHR21297; PTHR21297; 1.
DR   Pfam; PF03874; RNA_pol_Rpb4; 1.
DR   SMART; SM00657; RPOL4c; 1.
DR   SUPFAM; SSF47819; SSF47819; 1.
PE   1: Evidence at protein level;
KW   3D-structure; DNA-directed RNA polymerase; Nucleus; Reference proteome;
KW   Transcription.
FT   CHAIN           1..135
FT                   /note="DNA-directed RNA polymerase II subunit rpb4"
FT                   /id="PRO_0000073983"
SQ   SEQUENCE   135 AA;  15362 MW;  61C98593A01C1E04 CRC64;
     MPRAIFEEDA AQLKLGPEFE NEDMLTVSEA KILIETVLAQ RARETNGEIP MTDVMKKTVA
     YFNVFARFKT AEATYACERI LGNRFHKFER AQLGTLCCED AEEARTLIPS LANKIDDQNL
     QGILDELSTL RKFQD