ID   RPB5_ASFB7              Reviewed;         205 AA.
AC   Q65181;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 84.
DE   RecName: Full=DNA-directed RNA polymerase RPB5 homolog {ECO:0000303|PubMed:23041356, ECO:0000303|PubMed:32725217};
DE            Short=RPB5 homolog {ECO:0000305};
GN   OrderedLocusNames=Ba71V-108; ORFNames=D205R;
OS   African swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V)
OS   (ASFV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Asfuvirales; Asfarviridae; Asfivirus.
OX   NCBI_TaxID=10498;
OH   NCBI_TaxID=6937; Ornithodoros (relapsing fever ticks).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=11831707; DOI=10.1006/viro.1995.1149;
RA   Yanez R.J., Rodriguez J.M., Nogal M.L., Yuste L., Enriquez C.,
RA   Rodriguez J.F., Vinuela E.;
RT   "Analysis of the complete nucleotide sequence of African swine fever
RT   virus.";
RL   Virology 208:249-278(1995).
RN   [2]
RP   REVIEW.
RX   PubMed=23041356; DOI=10.1016/j.virusres.2012.09.014;
RA   Rodriguez J.M., Salas M.L.;
RT   "African swine fever virus transcription.";
RL   Virus Res. 173:15-28(2013).
RN   [3]
RP   SUBCELLULAR LOCATION.
RX   PubMed=30185597; DOI=10.1128/jvi.01293-18;
RA   Alejo A., Matamoros T., Guerra M., Andres G.;
RT   "A Proteomic Atlas of the African Swine Fever Virus Particle.";
RL   J. Virol. 92:0-0(2018).
RN   [4]
RP   REVIEW.
RX   PubMed=32725217; DOI=10.1042/bst20191108;
RA   Cackett G., Sykora M., Werner F.;
RT   "Transcriptome view of a killer: African swine fever virus.";
RL   Biochem. Soc. Trans. 48:1569-1581(2020).
CC   -!- FUNCTION: Component of the DNA-directed RNA polymerase (RNAP) that
CC       catalyzes the transcription in the cytoplasm of viral DNA into RNA
CC       using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000250|UniProtKB:P19388}.
CC   -!- SUBUNIT: Part of the viral DNA-directed RNA polymerase that consists of
CC       8 polII-like subunits (RPB1, RPB2, RPB3, RPB5, RPB6, RPB7, RPB9,
CC       RPB10), a capping enzyme and a termination factor.
CC       {ECO:0000303|PubMed:32725217}.
CC   -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000305}. Virion
CC       {ECO:0000269|PubMed:30185597}. Note=Found in association with viral
CC       nucleoid. {ECO:0000269|PubMed:30185597}.
CC   -!- SIMILARITY: Belongs to the archaeal RpoH/eukaryotic RPB5 RNA polymerase
CC       subunit family. {ECO:0000305}.
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DR   EMBL; U18466; AAA65337.1; -; Genomic_DNA.
DR   RefSeq; NP_042801.1; NC_001659.2.
DR   SMR; Q65181; -.
DR   GeneID; 22220337; -.
DR   KEGG; vg:22220337; -.
DR   Proteomes; UP000000624; Genome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   GO; GO:0019083; P:viral transcription; IEA:UniProtKB-KW.
DR   Gene3D; 3.90.940.20; -; 1.
DR   InterPro; IPR000783; RNA_pol_subH/Rpb5_C.
DR   InterPro; IPR035913; RPB5-like_sf.
DR   Pfam; PF01191; RNA_pol_Rpb5_C; 1.
DR   SUPFAM; SSF55287; SSF55287; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Host cytoplasm; Reference proteome;
KW   Transcription; Viral transcription; Virion.
FT   CHAIN           1..205
FT                   /note="DNA-directed RNA polymerase RPB5 homolog"
FT                   /id="PRO_0000373122"
SQ   SEQUENCE   205 AA;  23664 MW;  C601CAA278EE4374 CRC64;
     MAMQKLFTYI YEFIEYRKMV LLEEKVPYDK FVQMVLNTGF FRINAETLNH GIVSVFIFGA
     NGKYVHHGGD MRTLLTNTLN EKKHYEELIL IVDKPVLSKK NILDIIVEQR AANPTIVINI
     YPYHLFCINI PKVSAIPKHK LITQEEAQEF LGREYLQPQD LMQISASDPP VVWLGGRPGD
     FVQIERPSET AMHAVVIRFI TKSKI