ID   RPB7_ASFB7              Reviewed;         339 AA.
AC   Q89907;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 75.
DE   RecName: Full=DNA-directed RNA polymerase RPB7 homolog {ECO:0000303|PubMed:23041356, ECO:0000303|PubMed:32725217};
DE            Short=RPB7 homolog {ECO:0000305};
GN   OrderedLocusNames=Ba71V-105; ORFNames=D339L;
OS   African swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V)
OS   (ASFV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Asfuvirales; Asfarviridae; Asfivirus.
OX   NCBI_TaxID=10498;
OH   NCBI_TaxID=6937; Ornithodoros (relapsing fever ticks).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8262374; DOI=10.1016/0378-1119(93)90090-p;
RA   Yanez R.J., Rodriguez J.M., Boursnell M.E., Rodriguez J.F., Vinuela E.;
RT   "Two putative African swine fever virus helicases similar to yeast 'DEAH'
RT   pre-mRNA processing proteins and vaccinia virus ATPases D11L and D6R.";
RL   Gene 134:161-174(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=11831707; DOI=10.1006/viro.1995.1149;
RA   Yanez R.J., Rodriguez J.M., Nogal M.L., Yuste L., Enriquez C.,
RA   Rodriguez J.F., Vinuela E.;
RT   "Analysis of the complete nucleotide sequence of African swine fever
RT   virus.";
RL   Virology 208:249-278(1995).
RN   [3]
RP   REVIEW.
RX   PubMed=23041356; DOI=10.1016/j.virusres.2012.09.014;
RA   Rodriguez J.M., Salas M.L.;
RT   "African swine fever virus transcription.";
RL   Virus Res. 173:15-28(2013).
RN   [4]
RP   SUBCELLULAR LOCATION.
RX   PubMed=30185597; DOI=10.1128/jvi.01293-18;
RA   Alejo A., Matamoros T., Guerra M., Andres G.;
RT   "A Proteomic Atlas of the African Swine Fever Virus Particle.";
RL   J. Virol. 92:0-0(2018).
RN   [5]
RP   REVIEW.
RX   PubMed=32725217; DOI=10.1042/bst20191108;
RA   Cackett G., Sykora M., Werner F.;
RT   "Transcriptome view of a killer: African swine fever virus.";
RL   Biochem. Soc. Trans. 48:1569-1581(2020).
RN   [6]
RP   INDUCTION.
RX   PubMed=32075923; DOI=10.1128/jvi.00119-20;
RA   Cackett G., Matelska D., Sykora M., Portugal R., Malecki M., Baehler J.,
RA   Dixon L., Werner F.;
RT   "The African Swine Fever Virus Transcriptome.";
RL   J. Virol. 94:0-0(2020).
CC   -!- FUNCTION: Component of the DNA-directed RNA polymerase (RNAP) that
CC       catalyzes the transcription in the cytoplasm of viral DNA into RNA
CC       using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000250|UniProtKB:P62487}.
CC   -!- SUBUNIT: Part of the viral DNA-directed RNA polymerase that consists of
CC       8 polII-like subunits (RPB1, RPB2, RPB3, RPB5, RPB6, RPB7, RPB9,
CC       RPB10), a capping enzyme and a termination factor.
CC       {ECO:0000303|PubMed:32725217}.
CC   -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000305}. Virion
CC       {ECO:0000269|PubMed:30185597}. Note=Found in association with viral
CC       nucleoid. {ECO:0000269|PubMed:30185597}.
CC   -!- INDUCTION: Expressed in the early phase of the viral replicative cycle.
CC       {ECO:0000269|PubMed:32075923}.
CC   -!- DOMAIN: Contains an extended C-terminus, with no homology to
CC       characterized proteins. {ECO:0000303|PubMed:32725217}.
CC   -!- SIMILARITY: Belongs to the Asfivirus DNA-directed RNA polymerase RPB7
CC       homolog family. {ECO:0000305}.
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DR   EMBL; L10061; AAA42696.1; -; Genomic_DNA.
DR   EMBL; U18466; AAA65334.1; -; Genomic_DNA.
DR   PIR; JT0668; JT0668.
DR   RefSeq; NP_042798.1; NC_001659.2.
DR   PRIDE; Q89907; -.
DR   GeneID; 22220334; -.
DR   KEGG; vg:22220334; -.
DR   Proteomes; UP000000624; Genome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0019083; P:viral transcription; IEA:UniProtKB-KW.
PE   2: Evidence at transcript level;
KW   DNA-directed RNA polymerase; Early protein; Host cytoplasm;
KW   Reference proteome; Transcription; Viral transcription; Virion.
FT   CHAIN           1..339
FT                   /note="DNA-directed RNA polymerase RPB7 homolog"
FT                   /id="PRO_0000373653"
SQ   SEQUENCE   339 AA;  38751 MW;  5EA36C40D57B1D40 CRC64;
     MIDQKIFETT LNIDDPTNFC TNVEAHLLKE LENIYVGKCF KNSFILNITG VIQRSPCFIM
     RTNNSGRGYM HVRFSAVVSY LNAFDLIAAV KIIKNDSNII LGESLLTEPV TIVIPSSESQ
     NNVAEVGQIV PVQLANSSVY YIPGRQQASA TGSIFIPKHT FSVYHVQEEL TQEQALNLTK
     LVNIIEMLLE SRSKKDFKQI CFFEKLYYTY SISSDEILDL KIWKGPKGKE MSRLKPCNVL
     SFLYDALKNK SSSLGFWARP PNLLKSSPLA YQQDQNSFNA TELPIICSAE VMFVTLLKEI
     INYLQFMNDL CDTFNNEQLI KRHENIWMLI EQRKIGHDF