ID   RPB7_DICDI              Reviewed;         172 AA.
AC   Q54P04;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 98.
DE   RecName: Full=DNA-directed RNA polymerase II subunit rpb7;
DE            Short=RNA polymerase II subunit B7;
GN   Name=polr2g; Synonyms=rpb7; ORFNames=DDB_G0284891;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [2]
RP   IDENTIFICATION.
RX   PubMed=14704357; DOI=10.1093/nar/gkh163;
RA   Singh S.R., Rekha N., Pillai B., Singh V., Naorem A., Sampath V.,
RA   Srinivasan N., Sadhale P.P.;
RT   "Domainal organization of the lower eukaryotic homologs of the yeast RNA
RT   polymerase II core subunit Rpb7 reflects functional conservation.";
RL   Nucleic Acids Res. 32:201-210(2004).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       Component of RNA polymerase II which synthesizes mRNA precursors and
CC       many functional non-coding RNAs. Pol II is the central component of the
CC       basal RNA polymerase II transcription machinery. It is composed of
CC       mobile elements that move relative to each other. RPB7 is part of a
CC       subcomplex with RPB4 that binds to a pocket formed by RPB1, RPB2 and
CC       RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems
CC       to lock the clamp via RPB7 in the closed conformation thus preventing
CC       double-stranded DNA to enter the active site cleft. The RPB4-RPB7
CC       subcomplex binds single-stranded DNA and RNA (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Component of the RNA polymerase II (Pol II) complex consisting
CC       of 12 subunits. RPB4 and RPB7 form a subcomplex that protrudes from the
CC       10-subunit Pol II core complex (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit
CC       family. {ECO:0000305}.
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DR   EMBL; AAFI02000073; EAL64909.1; -; Genomic_DNA.
DR   RefSeq; XP_639913.1; XM_634821.1.
DR   AlphaFoldDB; Q54P04; -.
DR   SMR; Q54P04; -.
DR   STRING; 44689.DDB0216295; -.
DR   PaxDb; Q54P04; -.
DR   EnsemblProtists; EAL64909; EAL64909; DDB_G0284891.
DR   GeneID; 8624825; -.
DR   KEGG; ddi:DDB_G0284891; -.
DR   dictyBase; DDB_G0284891; rpb7.
DR   eggNOG; KOG3298; Eukaryota.
DR   HOGENOM; CLU_085878_2_0_1; -.
DR   InParanoid; Q54P04; -.
DR   OMA; MRQPGLG; -.
DR   PhylomeDB; Q54P04; -.
DR   Reactome; R-DDI-113418; Formation of the Early Elongation Complex.
DR   Reactome; R-DDI-674695; RNA Polymerase II Pre-transcription Events.
DR   Reactome; R-DDI-6781823; Formation of TC-NER Pre-Incision Complex.
DR   Reactome; R-DDI-6782135; Dual incision in TC-NER.
DR   Reactome; R-DDI-6782210; Gap-filling DNA repair synthesis and ligation in TC-NER.
DR   Reactome; R-DDI-6796648; TP53 Regulates Transcription of DNA Repair Genes.
DR   Reactome; R-DDI-6803529; FGFR2 alternative splicing.
DR   Reactome; R-DDI-6807505; RNA polymerase II transcribes snRNA genes.
DR   Reactome; R-DDI-72086; mRNA Capping.
DR   Reactome; R-DDI-72163; mRNA Splicing - Major Pathway.
DR   Reactome; R-DDI-72165; mRNA Splicing - Minor Pathway.
DR   Reactome; R-DDI-73776; RNA Polymerase II Promoter Escape.
DR   Reactome; R-DDI-73779; RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
DR   Reactome; R-DDI-75953; RNA Polymerase II Transcription Initiation.
DR   Reactome; R-DDI-75955; RNA Polymerase II Transcription Elongation.
DR   Reactome; R-DDI-76042; RNA Polymerase II Transcription Initiation And Promoter Clearance.
DR   Reactome; R-DDI-77075; RNA Pol II CTD phosphorylation and interaction with CE.
DR   Reactome; R-DDI-9018519; Estrogen-dependent gene expression.
DR   PRO; PR:Q54P04; -.
DR   Proteomes; UP000002195; Chromosome 4.
DR   GO; GO:0000932; C:P-body; IBA:GO_Central.
DR   GO; GO:0005665; C:RNA polymerase II, core complex; IBA:GO_Central.
DR   GO; GO:0016591; C:RNA polymerase II, holoenzyme; ISS:dictyBase.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IGI:dictyBase.
DR   GO; GO:0003697; F:single-stranded DNA binding; ISS:dictyBase.
DR   GO; GO:0003727; F:single-stranded RNA binding; IBA:GO_Central.
DR   GO; GO:0031369; F:translation initiation factor binding; IBA:GO_Central.
DR   GO; GO:0000291; P:nuclear-transcribed mRNA catabolic process, exonucleolytic; IBA:GO_Central.
DR   GO; GO:0060213; P:positive regulation of nuclear-transcribed mRNA poly(A) tail shortening; IBA:GO_Central.
DR   GO; GO:0045948; P:positive regulation of translational initiation; IBA:GO_Central.
DR   GO; GO:0006366; P:transcription by RNA polymerase II; IGI:dictyBase.
DR   GO; GO:0006367; P:transcription initiation from RNA polymerase II promoter; IBA:GO_Central.
DR   Gene3D; 2.40.50.140; -; 1.
DR   Gene3D; 3.30.1490.120; -; 1.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR036898; RNA_pol_Rpb7-like_N_sf.
DR   InterPro; IPR045113; Rpb7-like.
DR   InterPro; IPR005576; Rpb7-like_N.
DR   InterPro; IPR003029; S1_domain.
DR   PANTHER; PTHR12709; PTHR12709; 1.
DR   Pfam; PF00575; S1; 1.
DR   Pfam; PF03876; SHS2_Rpb7-N; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   SUPFAM; SSF88798; SSF88798; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleus; Reference proteome; Transcription.
FT   CHAIN           1..172
FT                   /note="DNA-directed RNA polymerase II subunit rpb7"
FT                   /id="PRO_0000327482"
SQ   SEQUENCE   172 AA;  18956 MW;  28070EDB09426BD7 CRC64;
     MFFHLTLEKD LHMHPKHCGP NLFTIATQQL YSEVEGTCTG RYGFIITITS VDFLSKGKVL
     ESSGYVVFNV KYKAIIFKPF KGEVLDAIVT KVTNLGFFAE AGPLSIFVST QLIPSDMIFD
     AQSAVPCFVS EDGSSKISKD DEVRLQIKGT RVDATEIFAI GSIREDYLGV IG