RPB7_SCHPO
ID RPB7_SCHPO Reviewed; 172 AA.
AC O14459;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 166.
DE RecName: Full=DNA-directed RNA polymerase II subunit rpb7;
DE Short=RNA polymerase II subunit B7;
GN Name=rpb7; ORFNames=SPACUNK4.06c, SPAPYUK71.02;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC STRAIN=972 / ATCC 24843, and JY741;
RX PubMed=9322754; DOI=10.1016/s0378-1119(97)00222-9;
RA Sakurai H., Ishihama A.;
RT "Gene organization and protein sequence of the small subunits of
RT Schizosaccharomyces pombe RNA polymerase II.";
RL Gene 196:165-174(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=9499374;
RA Shpakovski G.V., Lebedenko E.N.;
RT "Molecular cloning of rpb5+, rpb7+ and rpb11+ genes of the fission yeast
RT Schizosaccharomyces pombe: completing primary structure of all
RT indispensable subunits of its RNA polymerase II.";
RL Bioorg. Khim. 23:988-991(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [4]
RP INTERACTION WITH SEB1, AND MUTAGENESIS OF GLU-166 AND ASP-167.
RX PubMed=12907709; DOI=10.1093/nar/gkg688;
RA Mitsuzawa H., Kanda E., Ishihama A.;
RT "Rpb7 subunit of RNA polymerase II interacts with an RNA-binding protein
RT involved in processing of transcripts.";
RL Nucleic Acids Res. 31:4696-4701(2003).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC Component of RNA polymerase II which synthesizes mRNA precursors and
CC many functional non-coding RNAs. Pol II is the central component of the
CC basal RNA polymerase II transcription machinery. It is composed of
CC mobile elements that move relative to each other. RPB7 is part of a
CC subcomplex with RPB4 that binds to a pocket formed by RPB1, RPB2 and
CC RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems
CC to lock the clamp via RPB7 in the closed conformation thus preventing
CC double-stranded DNA to enter the active site cleft. The RPB4-RPB7
CC subcomplex binds single-stranded DNA and RNA (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Component of the RNA polymerase II (Pol II) complex consisting
CC of 12 subunits. RPB4 and RPB7 form a subcomplex that protrudes from the
CC 10-subunit Pol II core complex (By similarity). Interacts with seb1.
CC {ECO:0000250, ECO:0000269|PubMed:12907709}.
CC -!- INTERACTION:
CC O14459; Q9UTE3: seb1; NbExp=3; IntAct=EBI-608029, EBI-608019;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit
CC family. {ECO:0000305}.
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DR EMBL; D89594; BAA22804.1; -; Genomic_DNA.
DR EMBL; D86554; BAA22802.1; -; mRNA.
DR EMBL; AF027821; AAB92516.1; -; mRNA.
DR EMBL; AF055916; AAC99317.1; -; Genomic_DNA.
DR EMBL; CU329670; CAA20136.1; -; Genomic_DNA.
DR PIR; T39254; T39254.
DR RefSeq; XP_001713072.1; XM_001713020.2.
DR PDB; 3H0G; X-ray; 3.65 A; G/S=1-172.
DR PDB; 5U0S; EM; 7.80 A; g=1-172.
DR PDBsum; 3H0G; -.
DR PDBsum; 5U0S; -.
DR AlphaFoldDB; O14459; -.
DR SMR; O14459; -.
DR BioGRID; 278573; 123.
DR IntAct; O14459; 3.
DR MINT; O14459; -.
DR STRING; 4896.SPACUNK4.06c.1; -.
DR MaxQB; O14459; -.
DR PaxDb; O14459; -.
DR EnsemblFungi; SPACUNK4.06c.1; SPACUNK4.06c.1:pep; SPACUNK4.06c.
DR PomBase; SPACUNK4.06c; rpb7.
DR VEuPathDB; FungiDB:SPACUNK4.06c; -.
DR eggNOG; KOG3298; Eukaryota.
DR HOGENOM; CLU_085878_2_0_1; -.
DR InParanoid; O14459; -.
DR OMA; MRQPGLG; -.
DR PhylomeDB; O14459; -.
DR Reactome; R-SPO-113418; Formation of the Early Elongation Complex.
DR Reactome; R-SPO-5578749; Transcriptional regulation by small RNAs.
DR Reactome; R-SPO-674695; RNA Polymerase II Pre-transcription Events.
DR Reactome; R-SPO-6781823; Formation of TC-NER Pre-Incision Complex.
DR Reactome; R-SPO-6782135; Dual incision in TC-NER.
DR Reactome; R-SPO-6782210; Gap-filling DNA repair synthesis and ligation in TC-NER.
DR Reactome; R-SPO-6796648; TP53 Regulates Transcription of DNA Repair Genes.
DR Reactome; R-SPO-6807505; RNA polymerase II transcribes snRNA genes.
DR Reactome; R-SPO-72086; mRNA Capping.
DR Reactome; R-SPO-72165; mRNA Splicing - Minor Pathway.
DR Reactome; R-SPO-73776; RNA Polymerase II Promoter Escape.
DR Reactome; R-SPO-73779; RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
DR Reactome; R-SPO-75953; RNA Polymerase II Transcription Initiation.
DR Reactome; R-SPO-75955; RNA Polymerase II Transcription Elongation.
DR Reactome; R-SPO-76042; RNA Polymerase II Transcription Initiation And Promoter Clearance.
DR Reactome; R-SPO-77075; RNA Pol II CTD phosphorylation and interaction with CE.
DR Reactome; R-SPO-9018519; Estrogen-dependent gene expression.
DR EvolutionaryTrace; O14459; -.
DR PRO; PR:O14459; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0000932; C:P-body; IBA:GO_Central.
DR GO; GO:0005665; C:RNA polymerase II, core complex; IDA:PomBase.
DR GO; GO:0003697; F:single-stranded DNA binding; IBA:GO_Central.
DR GO; GO:0003727; F:single-stranded RNA binding; IBA:GO_Central.
DR GO; GO:0031369; F:translation initiation factor binding; IBA:GO_Central.
DR GO; GO:0000291; P:nuclear-transcribed mRNA catabolic process, exonucleolytic; IBA:GO_Central.
DR GO; GO:0060213; P:positive regulation of nuclear-transcribed mRNA poly(A) tail shortening; IBA:GO_Central.
DR GO; GO:0045948; P:positive regulation of translational initiation; IBA:GO_Central.
DR GO; GO:0010590; P:regulation of septum digestion after cytokinesis; IMP:PomBase.
DR GO; GO:0097394; P:telomeric repeat-containing RNA transcription by RNA polymerase II; IMP:PomBase.
DR GO; GO:0006367; P:transcription initiation from RNA polymerase II promoter; IBA:GO_Central.
DR Gene3D; 2.40.50.140; -; 1.
DR Gene3D; 3.30.1490.120; -; 1.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR036898; RNA_pol_Rpb7-like_N_sf.
DR InterPro; IPR045113; Rpb7-like.
DR InterPro; IPR005576; Rpb7-like_N.
DR InterPro; IPR003029; S1_domain.
DR PANTHER; PTHR12709; PTHR12709; 1.
DR Pfam; PF00575; S1; 1.
DR Pfam; PF03876; SHS2_Rpb7-N; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR SUPFAM; SSF88798; SSF88798; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-directed RNA polymerase; Nucleus; Reference proteome;
KW Transcription.
FT CHAIN 1..172
FT /note="DNA-directed RNA polymerase II subunit rpb7"
FT /id="PRO_0000073992"
FT MUTAGEN 166
FT /note="E->A: Reduced interaction with seb1."
FT /evidence="ECO:0000269|PubMed:12907709"
FT MUTAGEN 167
FT /note="D->A: Reduced interaction with seb1."
FT /evidence="ECO:0000269|PubMed:12907709"
SQ SEQUENCE 172 AA; 19103 MW; 0AECC46B9499FD33 CRC64;
MPFFLKELSL TISLHPSYFG PRMQDYLKAK LLADVEGTCS GQYGYIICVL DSNTIDIDKG
RVVPGQGFAE FEVKYRAVLW RPFRGEVVDA IVTTVNKMGF FANIGPLNVF VSSHLVPPDM
KFDPTANPPN YSGEDQVIEK GSNVRLKIVG TRTDATEIFA IATMKEDYLG VL
