RPC10_YEAST
ID RPC10_YEAST Reviewed; 110 AA.
AC Q04307; D6VS33; Q2VQW5;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 189.
DE RecName: Full=DNA-directed RNA polymerase III subunit RPC10;
DE Short=RNA polymerase III subunit C10;
DE AltName: Full=DNA-directed RNA polymerases III 12.5 kDa polypeptide;
DE AltName: Full=RNA polymerase III subunit C11;
GN Name=RPC11; OrderedLocusNames=YDR045C; ORFNames=YD9609.01C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CHARACTERIZATION.
RX PubMed=9869639; DOI=10.1101/gad.12.24.3857;
RA Chedin S., Riva M., Schultz P., Sentenac A., Carles C.;
RT "The RNA cleavage activity of RNA polymerase III is mediated by an
RT essential TFIIS-like subunit and is important for transcription
RT termination.";
RL Genes Dev. 12:3857-3871(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-96.
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=15905473; DOI=10.1093/nar/gki583;
RA Zhang Z., Dietrich F.S.;
RT "Mapping of transcription start sites in Saccharomyces cerevisiae using 5'
RT SAGE.";
RL Nucleic Acids Res. 33:2838-2851(2005).
RN [6]
RP REVIEW ON THE RNA POL III COMPLEX.
RX PubMed=10384303; DOI=10.1101/sqb.1998.63.381;
RA Chedin S., Ferri M.L., Peyroche G., Andrau J.-C., Jourdain S., Lefebvre O.,
RA Werner M., Carles C., Sentenac A.;
RT "The yeast RNA polymerase III transcription machinery: a paradigm for
RT eukaryotic gene activation.";
RL Cold Spring Harb. Symp. Quant. Biol. 63:381-389(1998).
RN [7]
RP FUNCTION.
RX PubMed=16362040; DOI=10.1038/sj.emboj.7600915;
RA Landrieux E., Alic N., Ducrot C., Acker J., Riva M., Carles C.;
RT "A subcomplex of RNA polymerase III subunits involved in transcription
RT termination and reinitiation.";
RL EMBO J. 25:118-128(2006).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC Component of RNA polymerase III which synthesizes small RNAs, such as
CC 5S rRNA and tRNAs. Involved in Pol III transcription reinitiation and
CC RNA cleavage during transcription termination.
CC {ECO:0000269|PubMed:16362040}.
CC -!- SUBUNIT: Component of the RNA polymerase III (Pol III) complex
CC consisting of 17 subunits.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus
CC {ECO:0000250|UniProtKB:P32529}.
CC -!- SIMILARITY: Belongs to the archaeal RpoM/eukaryotic RPA12/RPB9/RPC11
CC RNA polymerase family. {ECO:0000305}.
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DR EMBL; AF108188; AAD12060.1; -; Genomic_DNA.
DR EMBL; Z49209; CAA89076.1; -; Genomic_DNA.
DR EMBL; AY557636; AAS55962.1; -; Genomic_DNA.
DR EMBL; AY899253; AAX83938.1; -; mRNA.
DR EMBL; BK006938; DAA11893.1; -; Genomic_DNA.
DR PIR; S54031; S54031.
DR RefSeq; NP_010330.1; NM_001180353.1.
DR PDB; 3QT1; X-ray; 4.30 A; I=85-110.
DR PDB; 5FJ8; EM; 3.90 A; I=1-110.
DR PDB; 5FJ9; EM; 4.60 A; I=1-110.
DR PDB; 5FJA; EM; 4.65 A; I=1-110.
DR PDB; 6CNB; EM; 4.10 A; I=1-110.
DR PDB; 6CNC; EM; 4.10 A; I=1-110.
DR PDB; 6CND; EM; 4.80 A; I=1-110.
DR PDB; 6CNF; EM; 4.50 A; I=1-110.
DR PDB; 6EU0; EM; 4.00 A; I=1-110.
DR PDB; 6EU1; EM; 3.40 A; I=1-110.
DR PDB; 6EU2; EM; 3.40 A; I=1-110.
DR PDB; 6EU3; EM; 3.30 A; I=1-110.
DR PDB; 6F40; EM; 3.70 A; I=1-110.
DR PDB; 6F41; EM; 4.30 A; I=1-110.
DR PDB; 6F42; EM; 5.50 A; I=1-110.
DR PDB; 6F44; EM; 4.20 A; I=1-110.
DR PDB; 6TUT; EM; 3.25 A; I=1-110.
DR PDBsum; 3QT1; -.
DR PDBsum; 5FJ8; -.
DR PDBsum; 5FJ9; -.
DR PDBsum; 5FJA; -.
DR PDBsum; 6CNB; -.
DR PDBsum; 6CNC; -.
DR PDBsum; 6CND; -.
DR PDBsum; 6CNF; -.
DR PDBsum; 6EU0; -.
DR PDBsum; 6EU1; -.
DR PDBsum; 6EU2; -.
DR PDBsum; 6EU3; -.
DR PDBsum; 6F40; -.
DR PDBsum; 6F41; -.
DR PDBsum; 6F42; -.
DR PDBsum; 6F44; -.
DR PDBsum; 6TUT; -.
DR AlphaFoldDB; Q04307; -.
DR SMR; Q04307; -.
DR BioGRID; 32100; 303.
DR ComplexPortal; CPX-2660; DNA-directed RNA polymerase III complex.
DR DIP; DIP-887N; -.
DR IntAct; Q04307; 7.
DR MINT; Q04307; -.
DR STRING; 4932.YDR045C; -.
DR iPTMnet; Q04307; -.
DR MaxQB; Q04307; -.
DR PaxDb; Q04307; -.
DR PRIDE; Q04307; -.
DR EnsemblFungi; YDR045C_mRNA; YDR045C; YDR045C.
DR GeneID; 851615; -.
DR KEGG; sce:YDR045C; -.
DR SGD; S000002452; RPC11.
DR VEuPathDB; FungiDB:YDR045C; -.
DR eggNOG; KOG2906; Eukaryota.
DR GeneTree; ENSGT00550000075071; -.
DR HOGENOM; CLU_093932_3_0_1; -.
DR InParanoid; Q04307; -.
DR OMA; MVKFCPK; -.
DR BioCyc; YEAST:G3O-29658-MON; -.
DR Reactome; R-SCE-76061; RNA Polymerase III Transcription Initiation From Type 1 Promoter.
DR Reactome; R-SCE-76066; RNA Polymerase III Transcription Initiation From Type 2 Promoter.
DR PRO; PR:Q04307; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; Q04307; protein.
DR GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0005654; C:nucleoplasm; TAS:Reactome.
DR GO; GO:0005634; C:nucleus; IC:ComplexPortal.
DR GO; GO:0005666; C:RNA polymerase III complex; IDA:SGD.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006386; P:termination of RNA polymerase III transcription; IDA:ComplexPortal.
DR GO; GO:0006383; P:transcription by RNA polymerase III; IDA:ComplexPortal.
DR GO; GO:0006384; P:transcription initiation from RNA polymerase III promoter; IDA:ComplexPortal.
DR GO; GO:0042779; P:tRNA 3'-trailer cleavage; IEA:InterPro.
DR GO; GO:0042797; P:tRNA transcription by RNA polymerase III; IDA:SGD.
DR CDD; cd10509; Zn-ribbon_RPC11; 1.
DR InterPro; IPR019761; DNA-dir_RNA_pol-M_15_CS.
DR InterPro; IPR001529; DNA-dir_RNA_pol_M/15kDasu.
DR InterPro; IPR012164; Rpa12/Rpb9/Rpc10/TFS.
DR InterPro; IPR034014; Zn_ribbon_RPC11_C.
DR InterPro; IPR001222; Znf_TFIIS.
DR PANTHER; PTHR11239; PTHR11239; 1.
DR Pfam; PF02150; RNA_POL_M_15KD; 1.
DR Pfam; PF01096; TFIIS_C; 1.
DR PIRSF; PIRSF005586; RNApol_RpoM; 1.
DR SMART; SM00661; RPOL9; 1.
DR SMART; SM00440; ZnF_C2C2; 1.
DR PROSITE; PS01030; RNA_POL_M_15KD; 1.
DR PROSITE; PS51133; ZF_TFIIS_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-directed RNA polymerase; Metal-binding; Nucleus;
KW Reference proteome; Transcription; Zinc; Zinc-finger.
FT CHAIN 1..110
FT /note="DNA-directed RNA polymerase III subunit RPC10"
FT /id="PRO_0000121478"
FT ZN_FING 5..29
FT /note="C4-type"
FT /evidence="ECO:0000255"
FT ZN_FING 65..108
FT /note="TFIIS-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00472"
FT BINDING 69
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00472"
FT BINDING 75
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00472"
FT BINDING 100
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00472"
FT BINDING 103
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00472"
FT TURN 6..8
FT /evidence="ECO:0007829|PDB:6TUT"
FT STRAND 14..16
FT /evidence="ECO:0007829|PDB:6TUT"
FT HELIX 17..19
FT /evidence="ECO:0007829|PDB:6TUT"
FT STRAND 20..24
FT /evidence="ECO:0007829|PDB:6TUT"
FT STRAND 27..30
FT /evidence="ECO:0007829|PDB:6EU3"
SQ SEQUENCE 110 AA; 12513 MW; E4AA802EC9F92230 CRC64;
MLSFCPSCNN MLLITSGDSG VYTLACRSCP YEFPIEGIEI YDRKKLPRKE VDDVLGGGWD
NVDQTKTQCP NYDTCGGESA YFFQLQIRSA DEPMTTFYKC VNCGHRWKEN
