ID   RPC1_BP434              Reviewed;          95 AA.
AC   P16117;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   23-FEB-2022, entry version 120.
DE   RecName: Full=Repressor protein CI;
DE   Flags: Fragment;
GN   Name=CI;
OS   Enterobacteria phage 434 (Bacteriophage 434).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Siphoviridae; Lambdavirus.
OX   NCBI_TaxID=10712;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3031621; DOI=10.1093/nar/15.7.3181;
RA   Kuziel W.A., Tucker P.W.;
RT   "Determination of vector: insert junctions in lambda gt10 cDNAs that do not
RT   recut with EcoRI. Nucleotide sequence of the lambda imm434 HindIII-EcoRI
RT   DNA fragment encoding part of the cI protein.";
RL   Nucleic Acids Res. 15:3181-3181(1987).
RN   [2]
RP   X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 1-67.
RX   PubMed=2926803; DOI=10.1016/0022-2836(89)90375-6;
RA   Mondragon A., Subbiah S., Almo S.C., Drottar M., Harrison S.C.;
RT   "Structure of the amino-terminal domain of phage 434 repressor at 2.0-A
RT   resolution.";
RL   J. Mol. Biol. 205:189-200(1989).
RN   [3]
RP   X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 1-67.
RX   PubMed=3187531; DOI=10.1126/science.3187531;
RA   Aggarwal A.K., Rodgers D.W., Drottar M., Ptashne M., Harrison S.C.;
RT   "Recognition of a DNA operator by the repressor of phage 434: a view at
RT   high resolution.";
RL   Science 242:899-907(1988).
RN   [4]
RP   STRUCTURE BY NMR OF 1-67.
RX   PubMed=1311771; DOI=10.1016/0022-2836(92)90987-u;
RA   Neri D., Billeter M., Wuethrich K.;
RT   "Determination of the nuclear magnetic resonance solution structure of the
RT   DNA-binding domain (residues 1 to 69) of the 434 repressor and comparison
RT   with the X-ray crystal structure.";
RL   J. Mol. Biol. 223:743-767(1992).
RN   [5]
RP   STRUCTURE BY NMR.
RX   PubMed=9000626; DOI=10.1006/jmbi.1996.0692;
RA   Pervushin K., Billeter M., Siegal G., Wuethrich K.;
RT   "Structural role of a buried salt bridge in the 434 repressor DNA-binding
RT   domain.";
RL   J. Mol. Biol. 264:1002-1012(1996).
CC   -!- FUNCTION: Binds to two sets of three contiguous operator sites in the
CC       phage genome.
CC   -!- SUBUNIT: Homodimer, when bound to an operator.
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DR   EMBL; Y00118; CAA68301.1; -; Genomic_DNA.
DR   PDB; 1PER; X-ray; 2.50 A; L/R=2-70.
DR   PDB; 1PRA; NMR; -; A=2-70.
DR   PDB; 1R63; NMR; -; A=2-64.
DR   PDB; 1R69; X-ray; 2.00 A; A=2-70.
DR   PDB; 1RPE; X-ray; 2.50 A; L/R=2-70.
DR   PDB; 1SQ8; NMR; -; A=11-63.
DR   PDB; 2OR1; X-ray; 2.50 A; L/R=2-70.
DR   PDB; 2R63; NMR; -; A=2-64.
DR   PDBsum; 1PER; -.
DR   PDBsum; 1PRA; -.
DR   PDBsum; 1R63; -.
DR   PDBsum; 1R69; -.
DR   PDBsum; 1RPE; -.
DR   PDBsum; 1SQ8; -.
DR   PDBsum; 2OR1; -.
DR   PDBsum; 2R63; -.
DR   BMRB; P16117; -.
DR   SMR; P16117; -.
DR   EvolutionaryTrace; P16117; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   CDD; cd00093; HTH_XRE; 1.
DR   Gene3D; 1.10.260.40; -; 1.
DR   InterPro; IPR001387; Cro/C1-type_HTH.
DR   InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR   Pfam; PF01381; HTH_3; 1.
DR   SMART; SM00530; HTH_XRE; 1.
DR   SUPFAM; SSF47413; SSF47413; 1.
DR   PROSITE; PS50943; HTH_CROC1; 1.
PE   1: Evidence at protein level;
KW   3D-structure; DNA-binding; Repressor; Transcription;
KW   Transcription regulation.
FT   INIT_MET        1
FT                   /note="Removed; by host"
FT   CHAIN           2..>95
FT                   /note="Repressor protein CI"
FT                   /id="PRO_0000149714"
FT   DOMAIN          7..60
FT                   /note="HTH cro/C1-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00257"
FT   DNA_BIND        18..37
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00257"
FT   NON_TER         95
FT   HELIX           3..13
FT                   /evidence="ECO:0007829|PDB:1R69"
FT   HELIX           18..25
FT                   /evidence="ECO:0007829|PDB:1R69"
FT   HELIX           29..36
FT                   /evidence="ECO:0007829|PDB:1R69"
FT   TURN            37..39
FT                   /evidence="ECO:0007829|PDB:2OR1"
FT   HELIX           46..52
FT                   /evidence="ECO:0007829|PDB:1R69"
FT   HELIX           57..62
FT                   /evidence="ECO:0007829|PDB:1R69"
SQ   SEQUENCE   95 AA;  10426 MW;  6229D2DD66EA7EFC CRC64;
     MSISSRVKSK RIQLGLNQAE LAQKVGTTQQ SIEQLENGKT KRPRFLPELA SALGVSVDWL
     LNGTSDSNVR FVGHVEPKGK YPLISMVRAG SWCEA